Product Description

Affinity purified rabbit human IgG antibodies are developed using purified human IgG as the immunogen. The antibodies are conjugated to horse radish peroxidase (HRP) and are useful for detection, identification, quantization, isolation and localization of human immunoglobulins.

Species

Rabbit

Formulation

5 mg/mL in acetate (pH 7.2) with 0.01% NaN₃

Immunogen

Pure human IgG (whole molecule)

Purification

The antibody was affinity purified with human IgG immobilized on agarose and absorbed with BSA and mouse antibodies.

Conjugation

Reductive amination with HRP/Ab ratio - 2:1

Specificity

The antibody is specific against human IgG. It does not cross react with human serum albumin or mouse IgG.

Applications

ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL); IHC; IP

Scientific Description

IgG is a monomeric immunoglobulin. It is built of two heavy chains ? and two light chains. Each IgG has two antigen binding sites that bind with many pathogens including viruses, bacteria and fungi. It also assists in phagocytosis and neutralization of bacterial toxins.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one years from date of shipping if properly stored.

References

1. Junqueira, L. C. et al. Basic Histology, 2003 McGraw-Hill