Product Description

Progesterone is covalently linked to HRP through acylation. Progesterone-labelled HRP could be utilized for direct competitive ELISA for the detection and quantization of progesterone.

Direct competitive inhibition ELISA using immobilized anti-progesterone (3 µg/mL), and progesterone-labelled HRP (100 ng/mL). 50% inhibition occurred at 5 ng/mL of free progesterone under non-optimized conditions.

Structure of the Progesterone HRP Conjugate

Formulation

0.5 mg/mL in peroxidase stabilizing buffer with 50% glycerol

Conjugation

Progesterone-3(O-Carboxymethyl) Oxime-NHS active ester, via an acylation reaction

Progesterone/HRP molar ratio, approximay 3:1

Applications

ELISA

Scientific Description

Progesterone is a C-21 steroid hormone involved in the female menstrual cycle, pregnancy (supports gestation) and embryogenesis of humans and other species. Progesterone belongs to class of hormones called progestogens, and is the major naturally occurring human progestogen.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is three years from date of shipping if properly stored.