Product Description

This mouse monoclonal anti-AFB1 antibody was raised using AFB1-KLH conjugates as the immunogen. This antibody can be utilized for the development of competitive ELISA with paired antigen AFB1-BSA (ICP1203) and AFB1-HRP (ICP1206) conjugates for the detection and quantization of the food-borne mycotoxin AFB1.

Direct competitive inhibition ELISA using immobilized anti-AFB1 (ICP1204, 0.5 µg/µL) and AFB1 HRP (0.25 µg/mL), 50% inhibition occurred at 0.1 ng/mL free AFB1 in a non-optimized condition.

Structure of the immunogen and paired antigen

Species

Mouse

Formulation

1 mg/mL in PBS.

Paired Antigen

AFB1 o-methylcarboxylate  conjugated to BSA and HRP

Purification

Zinc sulfate precipitation

Specificity

The antibody recognizes aflatoxin B1 specifically; cross reactivity to other aflatoxins not tested yet.

Applications

Direct ELISA, IC50 is approximay  0.1 ng/mL

Scientific Description

Aflatoxin B1 is a food borne mycotoxin which is carcinogenic to both humans and animals. Contamination with AFB1 in food may be harmful to humans and animals.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C.Expiration date is 12 months from date of shipping if stored properly.