Product Description

The anti-phospho-tyrosine antibody is developed using phospho-tyrosine as the antigen and affinity-purified with phospho-tyrosine agarose. The purified antibody is immobilized to beaded agarose via strong thiol-ether linkages. This product could be utilized as an affinity matrix for rapid isolation and purification of proteins or peptides with phospho-tyrosine residues.

Immunoprecipitated samples with anti-phospho-tyrosine agarose (ICP9804).

Left: Mouse spleen lysate treated with complete adjuvant.

Right: Mouse spleen lysate without any treatment.

Detection: Phospho-tyrosine antibody, HRP (ICP9902)

Formulation

1 mL beaded agarose suspended in 2 mL slurry

Antibody Immobilized

5 mg/mL; antibody is covalently linked to CL-4B sepharose

Specificity

The antibody selectively captures peptides or proteins with phosphorylated tyrosine residues. It does not cross react with non-phosphorylated proteins.

Applications

Rapid isolation and purification of peptides or proteins with phospho-tyrosine residues from cell lysate or protease-digested mixtures.

Scientific Description

Protein phosphorylation is one of the most important events in post-translational modification. Protein tyrosine phosphorylation has been extensively studied and is believed to be one of the switches of cell signaling. Simple and effective tools for rapid isolation and purification of the phosphorylated species is essential for proteomic profiling of the phosphorylated proteins in different stages of development, disease, and signal transduction, as well as identifying the precise site of phosphorylation in a phospho-protein.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.