Catalog #

Product Description

ICP9805

The antibody is raised using the immunogenic conjugate phospho-tyrosine linked to KLH.  The pan-specific antibodies for phospho-tyrosine were specifically and selectively isolated from the immune serum using a unique technique which  makes sure the antibody can be competitively displaced by free phospho-tyrosine. This pan-specific anti-phospho-tyrosine (anti-pY) antibody could recognize the phospho-tyrosine residues on both proteins and  peptides. It has proved to be useful for affinity isolation, localization and detection of tyrosine phosphorylated proteins.  This antibody could be utilized for immunoprecipitation and affinity enrichment of tyrosine-phosphorylated proteins in cell and tissue lysates. It could be useful for immunoblotting tyrosine-phosphorylated proteins and ELISA quantization of protein tyrosine phosphorylation. It could also be used for non-radioactive tyrosine kinase assay.

The affinity isolation and enrichment of the tyrosine-phosphorylated species in mouse spleen lysates with anti-phospho-tyrosine antibody (ICP9805), followed by immuno-blotting detection with this antibody conjugated to HRP (ICP9902).

Immunohistochemistry analysis of human breast cancer with ICP9805

Species

Rabbit

Formulation

250 µg/mL in PBS, 50% glycerol, pH 7, 0.01% NaN₃

Immunogen

Phospho-tyrosine KLH conjugates

Purification

Affinity purified with phospho-tyrosine on agarose and competitively eluted with N-acetylated phospho-tyrosine

Specificity

The antibody recognizes proteins and peptides phosphorylated on tyrosine residues. There are no cross reactions with phospho-serine or threonine. Tested on human breast cancer tissue.

Applications

ELISA (1:1000); WB (1:250); IP (10 µg/500 µg protein sample); IHC (1:50); Non-radioactive Tyrosine Kinase Assay

Scientific Description

Tyrosine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to phospho-tyrosine which is considered a key step in the regulation of enzymatic activities such as signal transduction.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage,  store product at -20°C.  Expiration date is three years from date of shipping if stored properly.

References

1. Hoffhines, A. J. et al. J. Bio. Chem. 2006, 281, 37877-37887.doi:10.1074/jbc.M609398200