C918-GFP人眼脈絡(luò)黑色素瘤細(xì)胞-綠色標(biāo)記 處理步驟  

【Organism物種來源】Animal 
【Tissue組織來源】 
【Cell Type細(xì)胞類型】 
【Product Format產(chǎn)品狀態(tài)】 frozen/live culture
【Morphology細(xì)胞形態(tài)】 
【Culture Properties細(xì)胞特性】 
【Biosafety Level生物安全級別】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

【Disease細(xì)胞源疾病】
【Age年齡】 
【Gender性別】 Male/Female
【Storage Conditions保存條件】 liquid nitrogen vapor phase
【Karyotype染色體組型】 
【Images細(xì)胞圖片】 Cell Micrograph
【Derivation衍生細(xì)胞】
【Clinical Data臨床數(shù)據(jù)】 
【Comments其他描述】 
【Complete Growth Medium*培養(yǎng)基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. 
【Subculturing傳代方法】 
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation凍存條件】 
【Freeze Medium凍存培養(yǎng)基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存溫度】 Liquid nitrogen vapor phase
【Culture Conditions培養(yǎng)條件】 
【Atmosphere培養(yǎng)環(huán)境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培養(yǎng)溫度】 37°C
【STR Profile圖譜】 
【Population Doubling Time倍增殖時間】
【References參考文獻(xiàn)】

形態(tài)特性 上皮細(xì)胞樣  
生長特性 貼壁生長 
特征特性 CHO-HCV-C細(xì)胞整合有丙型肝炎病毒的Core基因，能穩(wěn)定表達(dá)C蛋白，是HCV基礎(chǔ)研究的*摸型。它由武漢大學(xué)病毒學(xué)國家重點(diǎn)實(shí)驗(yàn)室郭佳博士于2008年構(gòu)建并保存。 
中國倉鼠卵巢細(xì)胞（亞系克隆）；CHO-HCV-C培養(yǎng)條件 DMEM/F12(1:1): Ham's F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS  
傳代方法 1:3傳代,2-3天傳一代 
傳代情況 C15 
凍存條件 基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS  
細(xì)胞周期分為細(xì)胞間期和細(xì)胞分裂期，細(xì)胞間期較長，而細(xì)胞分裂期較短。
細(xì)胞間期是為細(xì)胞分裂的準(zhǔn)備時期，表現(xiàn)為細(xì)胞增大，營養(yǎng)物質(zhì)增多。細(xì)胞分裂期則是一個細(xì)胞由兩個細(xì)胞分解為一個細(xì)胞的過程。
細(xì)胞是生命的基本單位，細(xì)胞的特殊性決定了個體的特殊性，因此，對細(xì)胞的深入研究是揭開生命奧秘、改造生命和征服疾病的關(guān)鍵。細(xì)胞生物學(xué)已經(jīng)成為當(dāng)代生物科學(xué)中發(fā)展zui快的一門學(xué)科，是生物、農(nóng)學(xué)、醫(yī)學(xué)、畜牧、水產(chǎn)和許多生物相關(guān)專業(yè)的一門必修課程。
依據(jù)細(xì)胞種類和濃度，于無菌操作臺內(nèi)取 10 ml培養(yǎng)基加至 T25或 T75 flask中。取出已解凍之細(xì)胞懸浮液，緩緩加入T25 或T75 flask 內(nèi)之培養(yǎng)基， 混 合均勻，放入37 °C，5 % CO2 培養(yǎng)箱培養(yǎng)。