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人小細(xì)胞肺癌細(xì)胞NCI-H446

種屬	人
別稱(chēng)	H446; H-446; NCI-446; NCIH446
組織來(lái)源	肺，來(lái)源于轉(zhuǎn)移部位：胸腔積液
疾病	肋膜滲出癌，小細(xì)胞肺癌
傳代比例/細(xì)胞消化	1:2傳代,浮部分離心收集(1000RPM,5分鐘) ，貼壁部分消化1-2分鐘
培養(yǎng)基配置	RPMI1640培養(yǎng)基；10%胎牛血清；1%雙抗
簡(jiǎn)介	NCI-H446細(xì)胞株從一位小細(xì)胞肺癌患者的胸水中建立。細(xì)胞的原始形態(tài)并不具有小細(xì)胞肺癌特征。這個(gè)細(xì)胞株是小細(xì)胞肺癌的生化和形態(tài)學(xué)上的變種，表達(dá)神經(jīng)元的烯醇酶和腦部肌酸激酶同功酶。脫羧酶、蠶素、抗利尿激素、催產(chǎn)素或胃泌激素釋放肽未達(dá)到可檢測(cè)水平。C-myc DNA序列擴(kuò)增約20倍，c-myc RNA比正常細(xì)胞增加 15倍。最初傳代培養(yǎng)基用添加10 nM , 0.005 mg/ml 胰島素, 0.01 mg/ml 鐵傳遞蛋白, 10 nM 17- beta-雌二醇,30 nM 的RPMI 1640, 95% ，胎牛血清, 5%
形態(tài)	上皮細(xì)胞樣
生長(zhǎng)特征	貼壁，懸浮混合生長(zhǎng)
致瘤性	Yes, in nude mice (The cells form transplantable tumors with non-typical SCLC histology).
倍增時(shí)間	每周 2 至 3 次
保藏機(jī)構(gòu)	ATCC; HTB-171
備注	該細(xì)胞為半懸浮和半貼壁細(xì)胞，懸浮細(xì)胞離心收集，貼壁細(xì)胞消化處理

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