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人冠狀動(dòng)脈平滑肌細(xì)胞

種屬	人
組織來源	正常心臟組織
傳代比例	1:2傳代
培養(yǎng)基配置	基礎(chǔ)培養(yǎng)基500ml ；生長(zhǎng)添加劑5ml ；胎牛血清50ml ；雙抗5ml
簡(jiǎn)介	冠狀動(dòng)脈是供給心臟血液的動(dòng)脈，起于主動(dòng)脈根部，分左右兩支，行于心臟表面。冠狀動(dòng)脈疾病發(fā)生和發(fā)展的一個(gè)主要因素是由于血管平滑肌細(xì)胞轉(zhuǎn)變成為了具有繁殖能力的表型。近期的研究表明平滑肌細(xì)胞能表達(dá)鈣離子通道ICAM- 1和VCAM-1。其中ICAM-1和VCAM-1的表達(dá)可能是造成血管壁炎癥反應(yīng) ，并進(jìn)一步造成血管疾病的原因。因此，對(duì)冠狀動(dòng)脈血管平滑肌細(xì)胞的體外培養(yǎng)和研究可用來發(fā)現(xiàn)和確定新的冠狀血管疾病的靶向治療方法。
形態(tài)	長(zhǎng)梭狀細(xì)胞樣,不規(guī)則細(xì)胞樣
生長(zhǎng)特征	貼壁生長(zhǎng)
細(xì)胞檢測(cè)	平滑肌肌動(dòng)蛋白( α -SMA ）免疫熒光染色為陽性免疫熒光鑒定，細(xì)胞純度可達(dá)90%以上，不含有HIV-1、HBV、 HCV、支原體、細(xì)菌、酵母和真菌等。
倍增時(shí)間	每周 2 至 3 次
換液頻率	2-3天換液一次

between diklerent immune subtypes were screened through Wilcoxon rank sum test. The results showed that Clostridioides diklicile, ergillus fumigatus, Yarrowia lipolytica, and Fusarium pseudograminearum were all closely associated with the identified tumor immune subtypes. Our open-source software is freely available from GitHub at

acquisition of malignant phenotypes in cancer cells. Despite the well-established role of hypoxia in tumor cell invasion and metastasis, and resistance to therapy, relatively few studies have examined the contribution of hypoxia to cancer immune escape. Accumulating evidence reveals