成人做爰免费视频免费看_成人a级高清视频在线观看,成人a大片在线观看,成人a大片高清在线观看,成人av在线播放,一a一级片,一级黄 中国色 片,一级黄 色蝶 片,一级黄色 片生活片

產(chǎn)品展廳收藏該商鋪

您好 登錄 注冊(cè)

當(dāng)前位置:
世聯(lián)博研(北京)科技有限公司>技術(shù)文章>Biomomentum多軸機(jī)械測(cè)試儀Mach-1應(yīng)用:3D生物打印

技術(shù)文章

Biomomentum多軸機(jī)械測(cè)試儀Mach-1應(yīng)用:3D生物打印

閱讀:729          發(fā)布時(shí)間:2021-3-18

Biomomentum多軸機(jī)械測(cè)試儀Mach-1應(yīng)用:立體光刻3D生物打印方法制作人角膜基質(zhì)等效物。

生物醫(yī)學(xué)工程學(xué)年鑒,生物材料-工程細(xì)胞行為,已出版:2020年6月5日3D生物打印技術(shù)是用于角膜基質(zhì)組織再生的一種有前途的方法。在這項(xiàng)研究中,與角膜基質(zhì)細(xì)胞混合的甲基丙烯酸明膠(GelMA)被用作生物墨水。基于可見(jiàn)光的立體光刻(SLA)3D生物打印方法用于打印人類(lèi)角膜基質(zhì)的解剖學(xué)相似的圓頂形結(jié)構(gòu)。測(cè)試了兩種不同濃度的GelMA大分子單體(7.5和12.5%)的角膜基質(zhì)生物印記。由于高聚物濃度高,12.5%的GelMA比7.5%的GelMA堅(jiān)硬,這使其更易于處理。就生物印刷支架的水含量和透光率而言,我們觀察到具有12.5%GelMA濃度的支架更接近于天然角膜基質(zhì)組織。回到頂端|提供反饋信息的人隨后,細(xì)胞增殖,研究了在生物打印支架中封裝的人角膜基質(zhì)細(xì)胞的基因和蛋白質(zhì)表達(dá)。在第1天和第7天,觀察到在12.5%GelMA支架中的細(xì)胞相容性分別為81.86和156.11%,顯著高于7.5%GelMA支架中的細(xì)胞相容性。7天后在12.5%的GelMA樣品中觀察到角膜基質(zhì)細(xì)胞伸長(zhǎng),表明細(xì)胞附著,生長(zhǎng)和在支架內(nèi)的整合。與在塑料組織培養(yǎng)板上培養(yǎng)的細(xì)胞相比,在12.5%GelMA支架中培養(yǎng)的細(xì)胞的I型膠原蛋白,盧米肯和硫酸角質(zhì)素的基因表達(dá)隨時(shí)間增加。28天后,還使用免疫組織化學(xué)法觀察了I型膠原蛋白和lumican的表達(dá)。使用垂直軸的微機(jī)械測(cè)試儀(測(cè)定交聯(lián)時(shí)間MACH-cBiomomentum,加拿大)。

Abstract

3D bioprinting technology is a promising approach for corneal stromal tissue regeneration. In this study, gelatin methacrylate (GelMA) mixed with corneal stromal cells was used as a bioink. The visible light-based stereolithography (SLA) 3D bioprinting method was utilized to print the anatomically similar dome-shaped structure of the human corneal stroma. Two different concentrations of GelMA macromer (7.5 and 12.5%) were tested for corneal stroma bioprinting. Due to high macromer concentrations, 12.5% GelMA was stiffer than 7.5% GelMA, which made it easier to handle. In terms of water content and optical transmittance of the bioprinted scaffolds, we observed that scaffold with 12.5% GelMA concentration was closer to the native corneal stroma tissue. Subsequently, cell proliferation, gene and protein expression of human corneal stromal cells encapsulated in the bioprinted scaffolds were investigated. Cytocompatibility in 12.5% GelMA scaffolds was observed to be 81.86 and 156.11% at day 1 and 7, respectively, which were significantly higher than those in 7.5% GelMA scaffolds. Elongated corneal stromal cells were observed in 12.5% GelMA samples after 7 days, indicating the cell attachment, growth, and integration within the scaffold. The gene expression of collagen type I, lumican and keratan sulfate increased over time for the cells cultured in 12.5% GelMA scaffolds as compared to those cultured on the plastic tissue culture plate. The expression of collagen type I and lumican were also visualized using immunohistochemistry after 28 days. These findings imply that the SLA 3D bioprinting method with GelMA hydrogel bioinks is a promising approach for corneal stroma tissue biofabrication.

 

The crosslinking time was determined using a vertical axis micromechanical tester (Mach-1 v500c, Biomomentum Inc., Montreal, QC, Canada) by recording in situ gelation kinetics.

收藏該商鋪

請(qǐng) 登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

對(duì)比框

產(chǎn)品對(duì)比 產(chǎn)品對(duì)比 聯(lián)系電話 二維碼 意見(jiàn)反饋 在線交流

掃一掃訪問(wèn)手機(jī)商鋪
010-67529703
在線留言
主站蜘蛛池模板: 日韩av中文字幕| 中文字幕日韩熟女| 国产精品色情国产三级小说| 亚洲区色情区激情区小说色情书| 中文字字幕人妻中文| 日韩红色一级片| 午夜欧美精品久久久久久| 亚洲精品成人久久电影网| 韩国理论电影表妹| 国产在线观看91精品腿张开| a亚洲男人天堂| 夜夜爽妓女8888888视频| 久久久久精品国产14女子| 国产又黄又粗又爽又色的视频软件| 女教师精油按摩2| 国产精自产拍久久久久久蜜| 日韩在线亚洲精品| 国产欧美日韩综合精品一区| 男人天堂久久av| 日本在线理论片| 超碰日本爆乳中文字幕乱码| 国产日韩精品欧美一区喷水| 国产成人A∨激情视频厨房| 久久亚洲AV成人无码电影A片| 国产婷婷一区二区三区| 伦理片天堂eeuss影院| 国产免费久久精品国产传媒| 91蝌蚪九色在线| 欧美精品一区二区少妇免费A片| 亚洲欧美日韩不卡一区二区| 天堂…在线最新版天堂中文| 免费99精品国产自在现线| 3d肉蒲团快播种子| 韩国年轻的妈妈相亲高清视频| 日韩一级特黄毛片在线看| 欧美精品久久久久久久小说| 精品淑女少妇AV久久免费| bl肉yin荡受np各种play| AV天堂午夜精品一区| 亚洲欧美精品一区天堂久久| 师尊被掐着腰做到潮喷纯肉GB|