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DISUCCINIMIDYL SUBERATE （DSS）介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

DISUCCINIMIDYL SUBERATE （DSS）現(xiàn)貨供應(yīng)

DISUCCINIMIDYL SUBERATE （DSS）品牌介紹

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DISUCCINIMIDYL SUBERATE （DSS）僅用于科研

Thermo Scientific Pierce DSS, also called disuccinimidyl suberate, is noncleavable and membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide （NHS） ester at each end of an 8-carbon spacer arm.

NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine （K） residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.

DSS is first dissolved in an organic solvent such as DMF or DMSO, then added to the aqueous crosslinking reaction. BS3, the water soluble analog of DSS is also available for applications that require a hydrophilic crosslinker （e.g. to effect cell-surface crosslinking）. DSS and BS3 have essentially identical crosslinking activity toward primary amines.

DSS Highlights

• Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
• DSS crosslinker is membrane permeable, so intracellular crosslinking can be done
• High purity, crystalline DSS can be used to create high-purity conjugates