Peptide:
N-glycosidase F （PNGase F） is an asparagine amidase produced by Flavobacterium
meningosept-icum that serves as a useful tool in the research on protein
N-glycosylation. The cleavage site of PNGase F is the amide bond between
N-acetylglucosamine （GlcNAc） and aspartate residues on the medial side of the
glycoprotein, and converts aspartyl to aspartic acid on the enzymolysis
protein. This productoverexpressed in E.coli
and used for complete deglycosylation of antibodies and their associated
proteins