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化工儀器網>產品展廳>試劑標物>行業專用試劑>生物試劑> α-Gal Epitope (Galα1-3Galβ1-4GlcNAc...

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α-Gal Epitope (Galα1-3Galβ1-4GlcNAc-R) monoclonal

參考價 6683.25
訂貨量 ≥1
具體成交價以合同協議為準

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       欣博盛生物科技有限公司是一家致力于生命科學和生物技術領域的高科技企業。公司由在國內科研試劑領域有著十幾年從業經驗的專業技術團隊和企業管理團隊組建而成,專門從事以抗體、細胞因子、免疫檢測試劑盒等免疫學產品為主的生物試劑的研發與銷售。公司代理40余家歐美*生物技術公司的相關產品,能為生命科學多研究領域的廣大科研人員提供數萬種抗體、數千種蛋白以及相關檢測試劑盒等。經過幾年的不懈努力,欣博盛生物科技有限公司已成為免疫學、細胞生物學、分子生物學等領域科研產品的*供應商。同時,公司自主研發的ELISA試劑盒,品質*,行業*;在國內眾多重點實驗室廣泛使用,深受廣大科研人員的好評。欣博盛品牌ELISA試劑盒自推出以來已先后在Nature,PNAS, Cell research等雜志文章中被引用。

      公司不但擁有一批具有扎實生命科學基礎知識又具有豐富產品知識的市場人員,還具有一支訓練有素的技術支持團隊。所有技術支持人員均擁有國內重點大學生物學、醫學等相關專業碩士及以上學位,隨時為您提供技術咨詢和售后服務。

     欣博盛生物科技有限公司秉承“專業鍛造品質,服務打造市場”的經營理念, 本著“ 誠信*、服務質量*、客戶利益*” 的企業發展宗旨,為國內廣大客戶提供專業的前沿資訊、優質的產品、合理的價格、便捷的物流服務。

     欣博盛生物也將以強大的專業背景、優質的客戶服務、完備的產品供應和深厚的人脈資源,在同行業中保持良好的企業形象和競爭優勢。并將不斷把上優質的產品引入國內市場,滿足國內廣大客戶的需求。在二十一世紀生命科學蓬勃發展的時代,欣博盛將緊跟生命科學的發展動態,為廣大科研學者探索生命的奧秘而奉獻綿薄之力!

   我們的一切努力和付出,惟愿助您一臂之力!

 

 

 

 

ELISA試劑盒,抗體,蛋白/肽,分子化合物,IVD原料,特色試劑盒,其他試劑,耗材

供貨周期 兩周 規格 1*1Vial
貨號 ALX-801-090-1 應用領域 生物產業
主要用途 科研
Widely described antibody against α-galactose residues with broad cross-reactivity among different species. Useful for measuring the α-Gal epitope expression on cells, glycolipids and glycoproteins, characterization of hyperacute rejection (HAR) in organ and tissue transplantations and monitoring xenotransplantation experiments. The IgM isotype mimicks in vivo reactions and can be used for cytotoxicity assays for α-Gal specific pathways (addition or presence of complement necessary). Ideal as a high-throughput screening tool for inhibitors of antibody induced cytotoxicity.
Organ transplantation from pig to human result in HAR. Humans naturally produce large quantities of anti-α-Gal antibodies, which represent 1-3% of all circulating immunoglobulins and are produced by about 1% of all B cells. When pig organs or tissues are transplanted into the human body, the IgM isotype of anti-Gal binds to α-Gal epitopes, which causes activation of the complement cascade, resulting in cell lysis. This rapid activation of complement by anti-Gal IgM is an immunological barrier that poses the greatest risk of initiating HAR. The monoclonal antibody M86 to Galα1-3Gal epitopes developed by U. Galili, et al. is an essential tool in the study of human xenotransplantation and related research.

Product Details

Alternative Name:α-Galactose 1,3

Clone:M86

Host:Mouse

Isotype:IgM

Immunogen:Rabbit red blood cell membrane.

Source:Hybridoma tissue culture.

Species reactivity:Mouse, Rat
Porcine

Specificity:Recognizes synthetic and naturally produced mouse, rat and pig Galα1-3Gal epitopes on glycoproteins and glycolipids.

Crossreactivity:Does not cross-react with β-Gal glycoproteins or BSA.

Applications:ELISA, Flow Cytometry, IHC (PS), WB, FUNC

Recommended Dilutions/Conditions:Note: Do not use non-fat milk for blocking! Many blocking agents from non-human mammal sources will cross-react with the antibody.
Appropriate antibody dilution may vary from lot to lot. An initial dilution of 1:5 is recommended.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:Functional Application: can be used for cytotoxicity assays for α-Gal specific pathways

Quantity:4 mL per vial

Formulation:Liquid. Tissue culture supernatant.

Use/Stability:Stable for at least 3 years when stored at -20°C.

Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Shipping:Shipped on Dry Ice

Long Term Storage:-20°C

Technical Info/Product Notes:Cited samples:
For an overview on cited samples please click here.

Regulatory Status:RUO - Research Use Only


Product Literature References

Allergic response to medical products in patients with alpha-gal syndrome: K.V. Kuravi, et al.; J. Thorac. Cardiovasc. Surg. (2021), Abstract;
Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis: I. Pacheco, et al.; Pharmaceuticals 14, 635 (2021), Abstract;
Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer: M.K. Ristivojevic, et al.; Int. J. Mol. Sci. 16, 5742 (2020), Abstract;
Vaccination With Alpha-Gal Protects Against Mycobacterial Infection in the Zebrafish Model of Tuberculosis: I. Pacheco, et al.; Vaccines (Basel) 8, 195 (2020), Abstract; Full Text
A porcine xenograft‐derived bone scaffold is a biocompatible bone graft substitute: An assessment of cytocompatibility and the alpha‐Gal epitope: D.N. Bracey, et al.; Xenotransplantation 26, e12534 (2019), Abstract;
Effects of gamma radiation sterilization on the structural and biological properties of decellularized corneal xenografts: M.M. Islam, et al.; Acta Biomater. 96, 330 (2019), Abstract;
Tick Bites Induce Anti-α-Gal Antibodies in Dogs: A. Hodzic, et al.; Vaccines 7, E114 (2019), Abstract;
A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application: Y. Lu, et al.; Sci. Rep. 8, 15424 (2018), Abstract; Full Text
Biomaterial characterization of off-the-shelf decellularized porcine pericardial tissue for use in prosthetic valvular applications: J.A. Choe, et al.; J. Tissue Eng. Regen. Med. 12, 1608 (2018), Abstract; Full Text
Gal epitope expression and immunological properties in iGb3S deficient mice: A. Shao, et al.; Sci. Rep. 8, 15433 (2018), Abstract; Full Text
α-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells: M.K. Ristivojevic; Sci. Rep. 8, 12684 (2018), Abstract; Full Text
Alpha-gal is a possible target of IgE-mediated reactivity to antivenom: J. Fischer, et al.; Allergy 72, 764 (2017), Application(s): Western Blot using snake antivenoms, Abstract;
Recellularization of a novel off-the-shelf valve following xenogenic implantation into the right ventricular outflow tract: R.S. Hennessy, et al.; PLoS One 12, e0181614 (2017), Application(s): IHC using porcine sample, Abstract; Full Text
Efficient generation of GGTA1-null Diannan miniature pigs using TALENs combined with somatic cell nuclear transfer: W. Cheng, et al.; Reprod. Biol. Endocrinol. 14, 77 (2016), Abstract; Full Text
Feasibility of pig and human-derived aortic valve interstitial cells seeding on fixative-free decellularized animal pericardium: R. Santoro, et al.; J. Biomed. Mater. Res. B Appl. Biomater. 104, 345 (2016), Abstract;
In vivo xenogeneic scaffold fate is determined by residual antigenicity and extracellular matrix preservation: M.L. Wong, et al.; Biomaterials 92, 1 (2016), Application(s): Western blot, Abstract;
Immunoproteomics of processed beef proteins reveal novel galactose‐α‐1, 3‐galactose‐containing allergens: D. Apostolovic, et al.; Allergy 69, 1308 (2014), Abstract;
Laminin γ‐1 and collagen α‐1 (VI) chain are galactose‐α‐1, 3‐galactose–bound allergens in beef: H. Takahashi, et al.; Allergy 69, 199 (2014), Abstract;
Novel human renal proximal tubular cell line for the production of complex proteins: L. Fliedl, et al.; J. Biotechnol. 176, 29 (2014), Application(s): Flow cytometry, Abstract;
Highly Efficient Generation of GGTA1 Biallelic Knockout Inbred Mini-Pigs with TALENs: J. Xin, et al.; PLoS One 8, e84250 (2013), Abstract; Full Text
Possible role of a cell surface carbohydrate in evolution of resistance to viral infections in old world primates: I.A. Rodriguez, et al.; J. Virol. 87, 8317 (2013), Abstract;
Stepwise solubilization-based antigen removal for xenogeneic scaffold generation in tissue engineering: M.L. Wong, et al.; Acta Biomater. 9, 6492 (2013), Application(s): IHC on bovine tissue, Abstract;


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