產(chǎn)品概述

Synonyms ：DYKDDDDK tag;Flag-tag

同義詞 ：DYKDDDDK 標(biāo)簽；Flag-tag

Description：

A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK peptide.

描述：

一種融合標(biāo)簽，稱為DYKDDDK，由八個(gè)氨基酸（Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys）組成，包括一個(gè)腸激酶切割位點(diǎn)，專為免疫親和色譜設(shè)計(jì)。它允許在非變性條件下洗脫。針對(duì)該肽的幾個(gè)抗體已被開發(fā)出來(lái)。一種稱為M1的抗體在雙價(jià)金屬陽(yáng)離子存在下結(jié)合該肽，優(yōu)選Ca2+。通過螯合劑實(shí)現(xiàn)洗脫。另一種策略是使用過量的游離DYKDDDK肽進(jìn)行競(jìng)爭(zhēng)性洗脫。

Sequence： {ASP}{TYR}{LYS}{ASP}{ASP}{ASP}{ASP}{LYS}

Sequence： Shortening DYKDDDDK

Molecular： Formula C₄₁H₆₀N₁₀O₂₀

Molecular Weight ：1012.97

產(chǎn)品屬性

Purity ：> 95%

Solubility ：Soluble in Ultrapure water under 1mg/ml

Form： Lyophilized

Storage ：Store at -20°C.

純度： > 95%

溶解性 ：可溶于超純水，濃度小于1毫克/毫升

形態(tài) ：凍干粉

儲(chǔ)存 ：溫度保持在-20°C。