Note
L00436 interferes with IgG in mouse/rat serum and is not suitable for detecting mouse/rat serum samples.
產品概述
Product Description
GenScript His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged proteins. There are several potential usages:
●Quickly identify the presence of His-tagged proteins in samples.
●Optimize protein expression by monitoring the His-tagged proteins level.
●High throughput screening of stable cell lines expressing His-tagged proteins.
產品簡介
Detection Range ：1 ng/ml~729 ng/ml
Sensitivity： 1 ng/ml His-tagged proteins
Test Samples： N-terminal/C-terminal/internal His-tagged proteins4 x His/5 x His/6 x His-tagged proteins
Mammalian, yeast and bacteria cell lysates or cell supernatant
Conveniency ：Provide all reagents required for test
Complete test within 1.5 hours
Reagents compatibility： Tolerable with various reagents at certain concentration
Storage ：The unopened kit is stable for at least 12 months if stored at 2-8 °C and the opened kit may be stable for up to 1 month at 2-8 °C. Do not freeze the kit.
Kit Contents：
Component | Quantity | Part. No |
His Tag Plate | 1 plate (8 wells × 12 strips) | 436-80 |
Anti-His Monoclonal Antibody | 6 ml | 436-20 |
Antibody Tracer | 12 ml | 436-30 |
His Tag Standards (0,1,3,9,27,81,243,729 ng/ml) | 1 ml per each standard | 436-11,12,13,14,15,16,17,18 |
Assay Diluent | 60 ml | 436-60 |
20 × Wash Solution | 40 ml | B-A1-70 |
TMB Solution | 12 ml | B-A1-40 |
Stop Solution | 6 ml | B-A1-50 |
His Tag Standard Stock (10μg/ml) | 500 μl | 436-10 |
Plate Sealer | 2 | N/A |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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