Con A Sepharose 4B Ligand: Concanavalin A (Con A) Average Particle Size: 90 μm Matrix: 4% Agarose Particle Size: 45 µm-165 µm Ligand Concentration: 13 mg Con A/ml drained medium pH Stability Working Range: 4-9 pH Stability Cleaning-in-Place (CIP): 4-9 Pressure/Flow Specification: Base matrix 70-140 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d. Flow Velocity: < 75 cm/h at 25 ?C, HR 16/10 column, 5 cm bed height Storage Conditions: 2 to 8°C, 0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol Chemical Stability: Stable to all commonly used aqueous buffers Avoid Using: Chelating agents such as EDTA, 8 M urea or solutions having a pH below 3 should be avoided as these conditions results in removal of manganese from the lectin with loss of activity as a result. |