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產(chǎn)品描述
C1s enzyme is the activated form of C1s proenzyme. C1s is a subunit of the C1 complex which is the first complement component in the classical pathway of complement. C1s proenzyme is an inactive zymogen until C1 is activated. C1s
proenzyme is activated when C1 binds to and is activated by antibodies bound to antigens (immune complexes) yielding C1r enzyme, the first protease that initiates the cascade.
C1r enzyme in the C1 complex activates C1s proenzyme generating C1s enzyme. C1 complex is a non-covalent calcium-dependent complex of one C1q, two C1r and two C1s molecules. C1q binds by two or more of its six arms to the Fc domains of IgG or IgM. The binding of multiple arms to immune complexes causes the two C1r proteins in the complex (protease zymogens) to activate producing two proteases that cleave and activate the two C1s proenzymes in the complex. This activation of C1s proenzyme results in its cleavage into the two chain C1s enzyme with 58,000 and 28,000 dalton fragments.
Activated C1s enzyme cleaves complement component C4 releasing C4a and initiating covalent attachment of C4b to the activating surface. Activated C1s also cleaves C2 and the larger fragment of C2 binds to the surface-attached C4b forming C4b,C2a, the C3/C5 convertase of the classical pathway.
產(chǎn)品用途
C1s enzyme alone can be used to activate C4 or C2 but normallt it would do this while in complex with C1q. In the presence of Mg++ activated C4b and C2a can form fluid phase C4b,C2a the C3/C5 convertase of the classical pathway. In the presence of calcium C1s enzyme is still capable of binding to C1r and the complex is capable of binding to C1q. The resulting C1q-C1r-C1s complex is a fully active C1 molecule which will activate C4 and C2 in the fluid phase or on a cell bearing antibodies such as EA (Dodds, A.W. and Sim, R.B. (1997); Morgan, B.P. (2000)). EA are sheep erythrocytes with rabbit IgM anti-sheep erythrocyte antibodies bound to their surface (Morgan, B.P. (2000)).
產(chǎn)品性質(zhì)
Sizes Available:
250 µg/vial
Concentration:
1.0 mg/mL (see Certificate of Analysis for actual concentration)
Form:
Frozen liquid
Activity:
>90 % C1s will bind with excess C1-INH
Purity:
>90 % by SDS PAGE (Note: C1s enzyme is 86,000 unreduced, but
upon reduction runs as 58,000 and 28,000 chains on SDS PAGE)
Buffer:
10 mM sodium phosphate, 145 mM NaCl, pH 7.2
Extinction Coeff.
A280 nm = 0.96 at 1.0 mg/mL for pure C1s
Molecular Weight: 86,000 Da (2 chains)
Preservative:
None, 0.22 µm filtered.
Source:
Normal human serum (shown by certified tests to be negative
for HBsAg, HTLV-I/II, STS, and for antibodies to HCV, HIV-1
and HIV-II).
Precautions:
Use normal precautions for handling human blood products.
運(yùn)輸及保存方法
-70o C or below. Avoid freeze/thaw.
注意事項
This protein is purified from human serum and therefore precautions appropriate for handling any blood-derived product must be used even though the source was shown by certified tests to be negative for HBsAg, HTLV-I/II, STS, and for antibodies to HCV, HIV-1 and HIV-II.
| 貨號 | 品名 | 規(guī)格 | 品牌 |
| 78BFA10006-250 µg/vial | C1s Enzyme | 250 µg/vial | BIOHUB |
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