Interleukin-1 β （IL-1 β） Also known as endogenous mediators of white blood cells, monocytes, lymphocyte activating factors, etc., it is a cytokine protein encoded by the IL1B gene in humans. Interleukin-1 (IL-1) has two genes: IL-1 α And IL-1 β (This gene). IL-1 β Precursor cytoplasmic caspase 1 (interleukin-1 β Transformase cleavage to form mature IL-1 β。

Croyez Aimejie GMP IL-1 β# C01002 Details:

Source labeled with polyhistidine

Escherichia coli

No animal reagents and laboratories

According to GMP guidelines

Manufacturing and testing of endotoxin levels

Every 0 through the LAL method μ G protein<1.1 EU.

activity

Measured by its ability to induce IL-29 secretion in HT8 cells. The effect of Education Department 50 is<2.0 nanograms per milliliter.

Recombinant IL-1 β The specific activity is about>3 × 107IU/mg.

purity

>98% was determined by SDS-PAGE. Purified by Ni NTA chromatography.

formula

Freeze dry the protein from a solution containing 1X PBS and pH 8.0.

Re dissolution is recommended in sterile H

Freeze dried protein 2O to a concentration not less than 100 μ G/mL and incubate the stock solution for at least 20 minutes to ensure sufficient redissolution.

Freeze dried protein should be stored

At -20 ° C. This product can be stably stored for one year upon receipt, and processed and stored according to the instructions. After re dissolution, the protein sample should be stored at -20 ° C or -80 ° C. Avoid repeated freeze-thaw cycles.

be careful

Please use it within one month after protein re dissolution.

Croyez艾美捷GMP IL-1β#C01002詳細(xì)信息：

活性

通過(guò)其在HT8細(xì)胞中誘導(dǎo)IL-29分泌的能力來(lái)衡量。教育署50這種效果是<2.0納克/毫升。

重組人IL-1β的比活性約為>3×107IU/mg.

純度

>98% 由 SDS-PAGE 測(cè)定。通過(guò)Ni-NTA色譜法純化。

配方

將蛋白質(zhì)從含有1X PBS，pH 8.0的溶液凍干。

復(fù)溶建議在無(wú)菌H中復(fù)溶

凍干蛋白2O至濃度不低于100μg/ mL，并將儲(chǔ)備溶液孵育至少20分鐘以確保充分重新溶解。

儲(chǔ)存凍干蛋白應(yīng)儲(chǔ)存

在-20°C。 本產(chǎn)品在收到后可穩(wěn)定保存一年，并按照說(shuō)明進(jìn)行處理和儲(chǔ)存。復(fù)溶后，蛋白質(zhì)等分試樣應(yīng)儲(chǔ)存在-20°C或-80°C。 避免反復(fù)凍融循環(huán)。

注意

請(qǐng)?jiān)诘鞍踪|(zhì)復(fù)溶后一個(gè)月內(nèi)使用完畢。

GMP IL-1β相關(guān)研究：

→ IL-1β（白細(xì)胞介素-1β），人【C01002】

→ Croyez GMP IL-4（白細(xì)胞介素-4），人【C01006-GMP】

→ IL-4（白細(xì)胞介素-4），人【C01006】

→ Croyez GMP TGFβ1（轉(zhuǎn)化生長(zhǎng)因子β1），人【C01088-GMP】

→ 轉(zhuǎn)化生長(zhǎng)因子β1，人類【C01088】

→ IL-9（白細(xì)胞介素-9），人【C01011】

→ Croyez GMP IL-21（白細(xì)胞介素-21），人【C01026-GMP】

→ IL-21（白細(xì)胞介素-21），人【C01026】

→ Croyez GMP干擾素-γ（干擾素-γ），人【C01080-GMP】

→ 干擾素-γ（干擾素γ），人類【C01080】