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BioXcell：InVivoMAb anti-mouse F4/80
一 、產(chǎn)品介紹
F4/80是一種160 kDa的膜糖蛋白，屬于EGF-TM7家族。F4/80在大多數(shù)組織巨噬細(xì)胞上表達(dá)，包括腹膜、肺、腸、胸腺和脾紅髓巨噬細(xì)胞，以及Kuffer細(xì)胞、朗格漢斯細(xì)胞、骨髓基質(zhì)細(xì)胞和樹(shù)突細(xì)胞的亞群。F4/80的同源配體尚未確定，但在小鼠中的研究表明，F(xiàn)4/80對(duì)細(xì)胞因子的產(chǎn)生和誘導(dǎo)調(diào)節(jié)性T細(xì)胞是重要的。
二 、產(chǎn)品詳情
| 產(chǎn)品詳情 | |
| 產(chǎn)品貨號(hào) | BE0206 |
| 產(chǎn)品規(guī)格 | 1/5/25/50/100mg |
| 抗體亞型 | Rat IgG2b, κ |
| 推薦同型對(duì)照 | InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin（貨號(hào)：BE0090） |
| 推薦抗體稀釋液 | InVivoPure™ pH 7.0 Dilution Buffer（貨號(hào)：IP0070） |
| 免疫原 | C57BL/6 mouse thioglycollate stimulated peritoneal macrophages |
| 應(yīng)用 | in vivo Monocyte/Macrophage depletion/Functional assays/Immunohistochemistry (paraffin)/Immunohistochemistry (frozen)/Flow cytometry |
| 產(chǎn)品形式 | PBS , pH 7.0 Contains no stabilizers or preservatives |
| 內(nèi)毒素水平 | <2EU/mg (<0.002EU/μg)使用 LAL gel clotting 測(cè)定 |
| 純度 | >95% Determined by SDS-PAGE |
| 無(wú)菌處理 | 0.2 μM filtered |
| 生產(chǎn)形式 | 從組織培養(yǎng)上清液中純化得到。 |
| 純化形式 | Protein G |
| RRID | AB_10949019 |
| 分子量大小 | 150 kDa |
| 保存條件 | 抗體原溶液應(yīng)保存在4°C條件下，不要冷凍保存。 |
三 、已發(fā)表文獻(xiàn)
| 用途 | 已發(fā)表文獻(xiàn) |
| in vivo Monocyte/Macrophage depletion | Paul, S., et al. (2019). “Natural killer T cell activation increases iNOS(+)CD206(-) M1 macrophage and controls the growth of solid tumor.” J Immunother Cancer 7(1): 208 |
| in vivo Monocyte/Macrophage depletion | Wang, E. C. E., et al. (2019). “A Subset of TREM2(+) Dermal Macrophages Secretes Oncostatin M to Maintain Hair Follicle Stem Cell Quiescence and Inhibit Hair Growth.” Cell Stem Cell 24(4): 654-669.e656 |
| in vivo Monocyte/Macrophage depletion | Wang, W., et al. (2018). “RIP1 Kinase Drives Macrophage-Mediated Adaptive Immune Tolerance in Pancreatic Cancer.” Cancer Cell 34(5): 757-774 e757 |
| in vivo Monocyte/Macrophage depletion | Albacker, L. A., et al. (2013). “TIM-4, expressed by medullary macrophages, regulates respiratory tolerance by mediating phagocytosis of antigen-specific T cells.” Mucosal Immunol 6(3): 580-590 |
| Flow cytometry | Tittel, A. P., et al. (2012). “Functionally relevant neutrophilia in CD11c diphtheria toxin receptor transgenic mice.” Nat Methods 9(4): 385-390. |
| Flow cytometry | Winkler, I. G., et al. (2012). “Hematopoietic stem cell mobilizing agents G-CSF, cyclophosphamide or AMD3100 have distinct mechanisms of action on bone marrow HSC niches and bone formation.” Leukemia 26(7): 1594-1601 |
| in vivo Monocyte/Macrophage depletion | Bedoret, D., et al. (2009). “Lung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice.” J Clin Invest 119(12): 3723-3738. |
| Immunohistochemistry (paraffin) | Joffre, O., et al. (2008). “Prevention of acute and chronic allograft rejection with CD4+CD25+Foxp3+ regulatory T lymphocytes.” Nat Med 14(1): 88-92. |
| Immunohistochemistry (paraffin) | Lloyd, C. M., et al. (2008). “Three-colour fluorescence immunohistochemistry reveals the diversity of cells staining for macrophage markers in murine spleen and liver.” J Immunol Methods 334(1-2): 70-81 |
| Immunohistochemistry (paraffin) | Pull, S. L., et al. (2005). “Activated macrophages are an adaptive element of the colonic epithelial progenitor niche necessary for regenerative responses to injury.” Proc Natl Acad Sci U S A 102(1): 99-104 |
| Functional assays | Warschkau, H. and A. F. Kiderlen (1999). “A monoclonal antibody directed against the murine macrophage surface molecule F4/80 modulates natural immune response to Listeria monocytogenes.” J Immunol 163(6): 3409-3416 |
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