How does this work

Live-or-Dye Fixed Viability Stains are amine reactive dyes that are impermeable to cell membranes. Dyes can enter dead cells that have damaged membrane integrity and covalently label free amines on intracellular proteins. Dye labeling is very stable and can immobilize and penetrate cells without losing fluorescence or dye transfer between cells. Live-or-Dye The staining scheme has been optimized to maximize the differentiation of live/dead cells, with minimal staining of live cells to prevent interference with immune staining.

Biotium has multiple Live or Dye options Reactive dyes are available for selection, covering the fluorescence spectrum, and achieving maximum flexibility in designing multi-color flow plates.

Biotium also offers two sampler kits, each containing five different dye colors, for excitation from all popular flow cytometry laser lines. Live-or-Dye The Sampler Kit, Standard (32016) is designed for the most common flow cytometry laser and filter configurations, where dyes can be excited by UV, Violet, Blue, Yellow Green, and Red lasers. Live-or-Dye The Sampler Kit, Spectral (32017) is designed for spectral scanning flow cytometry. It contains dyes that can be excited by ultraviolet, violet, blue, and red lasers, all of which have been demonstrated in Cytek ®  The Aurora spectral cell analyzer was validated and selected due to its ability to fill many gaps in the spectral flow panel.

Live-or-Dye Fixed Viability Stains have dead cell specificity for all cell types, including mammalian cells, bacteria, and yeast.

UV/448可固定活性染色試劑盒允許通過流式細胞術或顯微鏡來區分活細胞和死細胞。在通過流式細胞術分析細胞表面蛋白表達時，活/死染色是有用的探針，因為它們允許將來自具有可滲透質膜的死細胞的細胞內熒光信號排除在分析之外。

艾美捷UV/448可固定活性染色試劑盒（200次測定）#32002組件：

Live-or-Dye™ 可固定死細胞染料

無水二甲基亞砜

采樣器套件組件

5 種不同的 Live-or-Dye™ 可固定死細胞染料

無水二甲基亞砜

UV/448可固定活性染色試劑盒特征：

高度死細胞特異性

用活性染料進行共價標記

承受固定和透化

多種顏色選擇

專為光譜流設計的染料選項

方便的采樣器套件

UV/448可固定活性染色試劑盒，涵蓋熒光光譜，在多色分析中實現非常大的靈活性。