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FHs 74 Int細胞人小腸上皮細胞

來源:生物試劑銷售中心   2019年08月28日 14:03  

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*/ 該細胞為ATCC源細胞,由試劑銷售引進 /*

FHs 74 Int細胞人小腸上皮細胞?

ATCCâ Number: CCL-241™

Lot Number: 70019552

 

Name: FHs 74 Int

Description: Small Intestine

Species: Human (Homo sapiens)

Volume/Ampule: Approximately 1 mL

Date Frozen: 09JAN2019

Recovery: A T-25 setup at a seeding density of 1.5 x 10viable cells/cmreaches approximately 15% confluence in 1 day and 90% confluence in 3 days.

Product Format: Cells cryopreserved in the appropriate cryopreservation medium Expiration Date:Not applicable

Storage Conditions: Vapor phase of liquid nitrogen

 

FHs 74 Int細胞人小腸上皮細胞?

 

 

Test / Method

Specification

Result

Ampule passage number

Report results

22

Population doubling level (PDL)

Report results

Not applicable

Total cells/ampule

(Cell count using Trypan Blue stain method)

Report results

4.4 x 10total cells/ampule

Post-freeze viability

(Cell count using Trypan Blue stain method)

≥ 50.0%

90.8%

Growth properties

(Visual observation method)

Adherent

Adherent

Morphology

(Visual observation method)

Epithelial-like*

Epithelial-like

Test for mycoplasma contamination

Hoechst DNA stain (indirect) method Agar culture (direct) method

PCR-based assay

 

None detected None detected None detected

 

None detected None detected None detected

Species determination: COI assay (interspecies)

Human

Human

 

 

ATCCâ Number: CCL-241™

Lot Number: 70019552

 

Species determination: STR analysis (intraspecies)

Human (Unique DNA Profile)

TH01: 7, 9.3

D5S818: 11, 12

D13S317: 11, 12

D7S820: 9, 10

D16S539: 11, 12

CSF1PO: 10, 11

Amelogenin: X vWA: 16, 18

TPOX: 8

Human (Unique DNA Profile)

TH01: 7, 9.3

D5S818: 11, 12

D13S317: 11, 12

D7S820: 9, 10

D16S539: 11, 12

CSF1PO: 10, 11

Amelogenin: X vWA: 16, 18

TPOX: 8

Sterility test (BacT/ALERT 3D)

iAST bottle (aerobic) at 32.5°C iNST bottle (anaerobic) at 32.5°C

 

No growth No growth

 

No growth No growth

Human pathogenic virus testing

(PCR-based assay for HIV, HepB, HPV, EBV, and CMV)

Report results

HIV – None detected HepB – None detected HPV – None detected EBV – None detected CMV – None detected

* Epithelial-like: Any adherent cells of a polygonal shape with clear, sharp boundaries between them.

 

 

Quality Assurance Specialist; Quality Assurance

 

ATCC hereby represents and warrants that the material provided under this certificate is pure and has been subjected to the tests and procedures specified and that the results described, along with any other data provided in this certificate, are true and correct to the best of the company’s knowledge and belief. This certificate does not extend to the growth and/or passage of any living organism or cell line beyond what is supplied within the container received from ATCC.

This product is intended to be used for laboratory research use only. It is not intended for use in humans, animals, or for diagnostics. Appropriate Biosafety Level (BSL) practices should always be used with this material. Refer to the Product Information Sheet for instructions on the correct use of this product.

ATCC products may not be resold, modified for resale, used to provide commercial services, or to manufacture commercial products without prior written agreement from ATCC.

© 2017 ATCC. The ATCC trademark and trade name are owned by the American Type Culture Collection.

 

FHs 74 Int細胞人小腸上皮細胞?

 

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