重組蛋白2-3
動物無™3重組蛋白
多克隆抗體4-5
單克隆抗體5-6
輔助熒光染色
試劑6
熒光標記抗體6-8
生物素化抗體9
多色流式細胞術檢測試劑盒10
酶聯免疫吸附試驗開發系統11
Fluorokine ®流式細胞儀套件11
干細胞培養及細胞套裝11
11二抗
Quantikine ® ELISA試劑盒12

2010年9月新產品
R＆D系統的細胞生物學研究™工具
新開發的MagCellect™人體血液
髓系樹突狀細胞分離試劑盒（目錄＃
MAGH120）的目的是隔離所有報道
髓系樹突狀細胞（MDC）的亞群從
人類血液樣本。大約百萬
mDCs每1億獲得單核細胞
處理。相對于其他現有的
包，豐富mDCs仍然無動于衷
抗體在整個隔離程序。
此外，這個工具包可以用來豐富的
教育署zui近CD16的身分+和CD56 +細胞亞群的mDC
（圖1）。功能分析
豐富mDCs置信度的RMS，分離細胞
是能夠誘導T細胞增殖的混合
（數據未顯示）白細胞反應。

The newly developed MagCellect™ Human Blood
Myeloid Dendritic Cell Isolation Kit （Catalog #
MAGH120） is designed to isolate all reported
myeloid dendritic cell （mDC） subpopulations from
human blood samples. Approximay one million
mDCs are obtained per 100 million PBMCs
processed. In contrast to other currently available
kits, enriched mDCs remain untouched by
antibodies throughout the isolation procedure.
In addition, this kit can be used to enrich for the
recently identifi ed CD16+ and CD56+ mDC subpopulations
（Figure 1）. Functional analysis of the
enriched mDCs confi rms that the isolated cells
are capable of inducing T cell proliferation in mixed
leukocyte reactions （Data not shown）.
References
1. Piccioli, D. et al. （2007） Blood 109:5371.
2. Morgado, J.M. et al. （2008） Transpl. Immunol. 19:55.
3. Rivas-Carvalho, A. et al. （2004） Int. Immunol. 16:1251.
4. Papewalis, C. et al. （2008） J. Immunol. 180:1462.
5. Gruenbacher, G. et al. （2009） Blood 114:4422.

Figure 1. Phenotypic Profi le of Enriched Human mDCs. The phenotypes of the mDCs recovered using the MagCellect Human Blood Myeloid Dendritic
Cell Isolation Kit （Catalog # MAGH120） are typically 7-31% BDCA1+/CD11c+ cells （A）, 10-38% BDCA3+/CD11c+ cells （B）, 15-31% CD16+/CD11c+ cells （C）1-3, and
10-24% CD56+/MHC-II+ cells （D）.4,5 Not shown: Plasmacytoid DCs （BDCA2+/CD45+）, which are not enriched. Diff erences in the fi nal percentages of the subpopulations
result from donor variability.

www.rndsystems.com/DAM_public/6782.pdf