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美國布魯克海文儀器公司>公司動態>Supported lipid bilayer repair mediated by AH peptide

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Supported lipid bilayer repair mediated by AH peptide

閱讀:221          發布時間:2017-6-26
 作者 Min Chul Kim,ab   Anders Gunnarsson,c   Seyed R. Tabaei,ab   Fredrik Höökc and   Nam-Joon Choabd

a School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore

b Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive 637553, Singapore

c Department of Applied Physics, Chalmers University of Technology, Gothenburg, Sweden

d School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive 637459, Singapore

 

摘要:The adsorption and fusion of small unilamellar lipid vesicles on silica-based substrates such as glass is a common method used to fabricate supported lipid bilayers. Successful bilayer formation depends on a number of experimental conditions as well as on the quality of the vesicle preparation. Inevitably, a small fraction of unruptured vesicles always remains in a supported bilayer, and this kind of defect can have devastating influences on the morphological and electrical properties of the supported bilayer when used as a biosensing platform. In this paper, a simple method is reported to improve the completeness of supported bilayers by adding a vesicle rupturing peptide as a final step in the fabrication process. Peptide treatment reduces the fraction of unruptured vesicles to less than 1%, as determined by epifluorescence microscopy and quartz crystal microbalance-dissipation experiments. This step can easily be incorporated into existing procedures for preparing high-quality supported lipid bilayers.

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