成人做爰免费视频免费看_成人a级高清视频在线观看,成人a大片在线观看,成人a大片高清在线观看,成人av在线播放,一a一级片,一级黄 中国色 片,一级黄 色蝶 片,一级黄色 片生活片

武漢原生原代生物醫(yī)藥科技有限公司

PriCells: Primary Culture of Pulmonary Arterial Smooth Muscle Cells

時(shí)間:2021-11-11 閱讀:240
分享:

PriCells: Primary Culture of Pulmonary Arterial Smooth Muscle Cells                            

1.Primary cultures of Pulmonary Arterial Smooth Muscle Cells (PASMCs) were isolated from human main pulmonary artery.
2.A segment of the main pulmonary artery was excised and placed in a sterile 10-cm dish containing primary cell culture media.
3.The segment was stripped of adventitia with a sterile forceps.
4.The main pulmonary artery segment was then cut longitudinally to open the vessel, and the endothelial layer was removed by gentle rubbing with a cell scraper.
5.The vessel was then cut into 2-mm segments, inverted, and placed on a collagen-coated 35-mm tissue culture dish.
6.A drop of primary cell culture media containing 10% fetal bovine serum, primary cell culture supplements, antibiotics, and antimycotics was then added, and the cells were grown overnight at 37°C in a humidified atmosphere with 5% CO2-95% air.
7.The next day, an additional 2 ml of complete medium were added.
8.The growth medium was subsequently changed every 2 days.
9.When SMC islands could be observed under the microscope, the tissue segment was removed and the individual cell islands were subcloned.
10.Identity was confirmed as PASMCs by immunostaining (>99% positive) with SMC actin. This was taken as evidence that cultures were not contaminated with fibroblasts or endothelial cells.
11.All cultures for subsequent experiments were maintained in primary cell culture media containing 10% fetal bovine serum, primary cell culture supplements, antibiotics, and antimycotics at 37°C in a humidified atmosphere with 5% CO2-95% air.

References
1.Chamley-Campbell, J., Campbell, G., and Ross, R.: The Smooth Muscle in Cell Culture, Physiol. Res. 59, 1, 1979.
2.Richardson, M., Taylor, D., Casey, M., MacDonald, P., and Stull, J.: Biochemical Markers of Contraction in Human Myometrial Smooth Muscle Cells in Culture, In Vitro Cell. Dev. Biol. 23, 21, 1987.
3.Eskin, S., Sybers, H., Lester, J., Navarro, L., Gotto, A., and DeBakey, M.: Human Smooth Muscle Cells Cultured From Atherosclerotic Plaques and Uninvolved Vessel Wall, In Vitro 17 (8), 713, 1981

會(huì)員登錄

×

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~
撥打電話 產(chǎn)品分類
在線留言
主站蜘蛛池模板: www.精品亚洲| 亚洲欧美强伦一区二区另类| 国产伦精品一区二区三区免.费| 中文字幕痴汉系列| 久久久久久久久久77777| 国产亚洲欧美日本一二三本道| 欧美日韩黄色| 久久久久久久国产精品亚洲| 欧美成人精品A片免费区网站| 亚洲av色福利天堂| 无码AV大香线蕉伊人久久| 无敌神马影视影院在线| 金珠演过的韩国大片| 国产精品AV无码毛片久久| 久久区亚洲欧美一区中文字幕| 婷婷色综合视频在线观看| 亚洲高清成人AV电影网站| 美女搜查官被痴汉侵犯耻辱| 免费精品国产人妻国语色戒| 蜜臀国产一区二区三区无码A片| 快穿做妓女好爽H| 91精品福利在线| 一二三区理论片| 中国内射XXXX6981少妇| 国产偷人爽久久久久久老妇APP| 中文字幕乱妇无码AV在线| 69成线在人线免费视频| 色情AV亚洲精品一区二区| 我吃了教官的J吧男男(H)| 婷婷激情综合色五月久久竹菊影视| 99久久中文字幕东| 亚洲欧美性都花花世界爱爱网| 久久久久久久一级片| 日本无码人妻一区二区色欲| 52欧美日日夜夜撸影院| 我被老外躁到了高潮八次| 色日本欧美三级色女| 城人电影wang| 公交车上少妇被躁爽| 国产免费啪嗒啪嗒视频看看| 欧美男女激情四射|