成人做爰免费视频免费看_成人a级高清视频在线观看,成人a大片在线观看,成人a大片高清在线观看,成人av在线播放,一a一级片,一级黄 中国色 片,一级黄 色蝶 片,一级黄色 片生活片

武漢原生原代生物醫藥科技有限公司

PriCells: Primary dermal fibroblasts isolated from human

時間:2021-10-29 閱讀:212
分享:

PriCells: Primary dermal fibroblasts isolated from human                  


Fibroblasts were established from donors of human normal skin, scar, and keloid using the explant method.

The specimens were washed with sterile phosphate-buffered saline (PBS), and the subcutaneous tissues were removed carefully.

The skins were then cut into small pieces (1-2 mm3).

After being digested with PriCells Isolation Kit III (or using 0.25% trypsin containing 0.05% ethylenediaminetetraacetic acid) at 4°C overnight, the epidermal layers were removed, and the remaining dermal parts were further digested with PriCells Isolation Kit III (or using 0.25% trypsin containing 0.05% ethylenediaminetetraacetic acid) at 37°C for another 4 hours.

The digested cells were then passed through a 70-µm cell strainer (BD Biosciences), centrifuged, and resuspended in PriCells primary cell culture system.

Cells were seeded on tissue culture plates at 1x103 cells/cm2, and maintained at 37°C with 5% CO2.

After 24 hours, the plates were washed with PBS to remove residual non-adherent cells.

The resulting adherent cells were grown to confluency within 7 days.

Cells were subcultured every 4 days using 0.25% trypsin/EDTA.


會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標簽:
保存成功

(空格分隔,最多3個,單個標簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復您~
撥打電話 產品分類
在線留言
主站蜘蛛池模板: 成人国产欧美大片一区| AV亚洲产国偷V产偷V自拍AV| 亚洲综合AV久久国产精品凡士林| 成年女美黄网站大全免费播放| 亚洲国产精品成人麻豆| 亚洲精品第一区二区APP| 国产视频精品免费| 韩国理论电影在线看| 中文字幕亚洲国产欧| 亚洲在线日韩在线| 色欲AV国产精品一区二区| 国产av天堂| 久久久网中文字幕| 精品久久久久久久久中文字幕| 欧美另类又黄又爽的A片| 亚洲va天堂va国产va久| 国产精品一区福利| 亚洲va久久久噜噜噜久久无| 欧美激情四射在线| AV色蜜桃一区二区三区| 少妇被阴内射XXXB少妇BB| 国产亚洲av综合人人澡| 国师受被肉到失禁各种PLAY| 日韩国产精品人妻无码久久久| 国产欧美另类久久久精品免费| 99国产精品热久久久久久| 亚州AV综合色区无码一区| 精品久久久久中文字幕一区| 麻豆AV字幕无码中文| 久久高清一级毛片| 69SEX久久精品国产麻豆| 日韩一欧美一级片| 久久这里只有精品1| 欧美日韩国产在线视频| 亚洲国产精品欧美| 国产精品久久久久5252| 欧美性生交XXXXX无码小说| 久久久精品理论A级A片| 成人av大香蕉| 日韩亚洲综合av| 古风一女N男到处做高H|