成人做爰免费视频免费看_成人a级高清视频在线观看,成人a大片在线观看,成人a大片高清在线观看,成人av在线播放,一a一级片,一级黄 中国色 片,一级黄 色蝶 片,一级黄色 片生活片

產品展廳收藏該商鋪

您好 登錄 注冊

當前位置:
蘇州守真儀器設備有限公司>技術文章>細菌培養方法

技術文章

細菌培養方法

閱讀:4794          發布時間:2020-8-6

細菌培養方法

1儀器:

K罩細菌過濾效率檢測儀、高壓蒸汽滅菌器、電子天平、生化培養箱、軌道式振蕩器、超潔凈工作臺、菌落計數器

  1. 試劑及材料:

蒸餾水、75%酒精、金黃色葡萄球菌ATCC 6538、胰蛋白酶大豆瓊脂(TSA)、胰蛋白胨大豆肉湯培養基(TSB)、蛋白胨水、酒精燈、90mm平皿、500ml錐形瓶

  1. TSA培養基的配制

取一個干凈的500ml的錐形瓶,稱取16g TSA,溶于400ml水中,包扎后放入高壓蒸汽滅菌器中滅菌(121℃-123℃,20min),滅菌結束后取出,待其冷卻至50℃-60℃時,將液體培養基逐個倒25ml左右入無菌培養皿中,操作應在超潔凈工作臺上進行,以酒精燈的火焰周圍作為無菌區域,避免雜菌污染。

待培養基冷卻凝固后,將其倒置放入恒溫培養箱中,37℃條件下培養24h,將有菌落生長的培養基舍棄不用,無雜菌生長的取出備用,盡量現用現做,如不能盡快使用,應密封放在4℃冰箱內冷藏,可保存3-4天。                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               

  1. 菌懸液的制備

將金黃色葡萄球菌ATCC6538接種在已滅菌好的100mlTSB(胰蛋白胨大豆肉湯液體培養基)中,在37℃震蕩培養24h。

用無菌移液槍取出上述培養好的菌液1ml注入9ml一滅菌好的蛋白胨水中,混勻,一次逐級稀釋10倍,稀釋至10-7(根據菌種實際情況來判斷需要稀釋至多少),然后分別取10-5、10-6、10-7菌液各0.1ml接種到備TSA培養基上,每個稀釋稀釋倍數做少4組平行,用涂布棒沿同一方向涂抹均勻,(不要涂抹到培養皿的邊緣上),放入生化培養箱中培養,培養溫度(37±2)℃,培養時間(24±2)h。培養結束后用菌落計數器計數,根據稀釋倍數確定原始菌液的濃度。

計算公式:原始濃度=(A/V)*D

A為培養皿上的平均菌落數

V為接種液的體積

D為稀釋倍數

按照上述方法確定原始菌液的濃度后,用1.5%的蛋白胨水將上述培養物稀釋至約5*105cfu/ml。

TSB的制備:稱取3gTSB, 溶于100ml水中,放入高壓蒸汽滅菌器中(121℃-123℃,20min)滅菌,冷卻后備用。

蛋白胨水的配制:稱取1.5g蛋白胨溶于100ml水中,包扎,放入高壓蒸汽滅菌器中(121℃-123℃,20min)滅菌,冷卻后備用。

實驗結束后逐級取出培養皿并蓋上皿蓋,標記后倒置放入恒溫培養箱中,37℃培養24-48h.試驗中,陽性對照組應進行至少兩次實驗,終陽性質控結果取平均值。

培養結束后,將所有培養皿取出,使用菌落計數器進行計數,并將每一級菌落數對應陽性孔轉換表進行轉換,轉換后的數值求和,即得出菌落總數,陽性質控值范圍應在(2200±500)cfu之間。

細菌過濾效率計算公式如下:

BFE=(C-T)/C*

式中:C為陽性質控平均值,T為實驗組菌落總和

收藏該商鋪

登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時間回復您~

對比框

產品對比 產品對比 聯系電話 二維碼 意見反饋 在線交流

掃一掃訪問手機商鋪
17321150489
在線留言
主站蜘蛛池模板: 被病娇做到哭H| 久久天堂网| 韩日三级免费电影| 女性高爱潮免费有声视频网站| 《乳色吐息》无删减在线观看风车| 亚州笫一色惰网站| 中文字幕人妻熟人| 中文字幕一区二区人妻秘书| 黑料吃瓜王-黑料吃瓜网-51吃瓜黑料| 日韩高清不卡一区二区三区| 人妻va精品va欧美va| 日韩国产精品久久久久久| 色婷婷AV一区二区牛牛影视| 国产精品美女久久久久久| 精品一区二区三区无码AV久久| 国产人妻精品久久久久久很牛| 欧美一区二区视频在线观看| 99热精品久久只有精品| 麻豆国产人妻精品无码AV| 国产自国产自愉自愉免费24区| 亚洲成人在线观看免费二区| 三A级做爰片免费观看国产电影| 日韩特级毛片免费观看视频| 亚洲中文字幕无码一去台湾| 免费黄色一级大片91| 免费精品一区二区三区A片在线| 99热久久这里只有精品| 激情偷拍欧美色图| 女人脱精光让人桶爽了| 日本国产精品无码字幕在线观看| 国产美女一区二区| 亚洲国产精品无码AV久久久| 免费又粗又硬进去好爽A片| 精品AV一区二区三区久久| 中文字幕亂倫免賛視頻| 欧美日韩久久久久| 国产欧美精品日韩| 老师你下面太紧进不去小黄文| 精品香蕉在线观看视频| 东京热一区二区三区无码视频| 久久久久久久久精品免费|