Kerafast抗體Anti-DNA-RNA Hybrid [S9.6] Antibody

產品介紹：

Kerafast的貨號：ENH001，Anti-DNA-RNA Hybrid [S9.6] Antibody，這種小鼠單克隆抗體是針對 ΦX174 噬菌體衍生的合成 DNA-RNA 抗原生成的，可識別各種長度的 RNA-DNA 雜合體。

特色：

可用于檢測 R 環

對 DNA-RNA 雜交體的高特異性和親和力

不與單鏈 DNA 或雙鏈 DNA 發生交叉反應

對于富含 AU 的雙鏈 RNA，觀察到了輕微的交叉反應（約 5 倍以下）。

長度為 8、10、15 和 23 個堿基對的雜交體顯示出高親和力結合

DNA-RNA 雜合體是真核細胞中的一種自然現象，這些雜合體的水平在具有高轉錄活性的位點增加，例如在轉錄起始、抑制和延伸期間。由于 RNA-DNA 雜合體會影響基因組的不穩定性，因此 S9.6 抗體是一種有用的試劑，可幫助研究在 DNA 復制或其他細胞過程中由這些雜合體形成的 R 環和損傷的后果。此外，S9.6 抗體可有效識別用于微陣列研究的 RNA-DNA 雜交。

This mouse monoclonal antibody was generated against a ΦX174 bacteriophage-derived synthetic DNA–RNA antigen and recognizes RNA-DNA hybrids of various lengths.

Highlights:

* Useful in the detection of R-loops

* High specificity and affinity for DNA-RNA hybrids

* Does NOT cross-react with single-stranded DNA or double-stranded DNA

* Minor cross-reaction (~5-fold less) has been observed for AU-rich double-stranded RNA.

* High affinity binding shown for hybrids of 8, 10, 15, and 23 base pairs in length

產品詳情：

Product Type:	Antibody
Name:	Anti-DNA-RNA Hybrid [S9.6]
Antigen:	S9.6 ΦX174 bacteriophage-derived synthetic DNA–RNA antigen
Isotype:	Rabbit IgG
Fusion Tag(s):	Mouse Fab version contains His-tag
Clone Name:	S9.6
Reactivity:	High specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids
Immunogen:	ΦX174 bacteriophage-derived synthetic DNA/RNA
Purification Method:	Protein A/G
Buffer:	ENHOO1: PBS, 0.05% (w/v) Sodium Azide Ab01137- : PBS with 0.02% Proclin 300
Tested Applications:	Dot Blot Analysis: 0.2 µg/mL. Affinity Binding Assay: Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected increased DNA RNA hybrids at four actively transcribed genes upon shRNA-mediated knockdown of BRCA1 or BRCA2, but not PCID2 or RAD51 in HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected R-loops formed over beta-actin gene using HeLa chromatin preparation. RNase H treatment of the chromatin preparation prevented clone S9.6 from immunoprecipitating target chromatin fragments (Skourti-Stathaki, K., et al. (2011). Mol. Cell. 42(6):794-805). Chromatin Immunoprecipitation-sequencing (ChIP-seq) Analysis: A representative lot detected genome-wide distribution of DNA-RNA hybrids in budding yeast by ChIP-seq analysis (El Hage, A., et al. (2014). PLoS Genet. 10(10):e1004716). Immunocytochemistry Analysis: Representative lots immunolocalized nuclear R loops by fluorescent immunocytochemistry staining of methanol-fixed H1 human embryonic stem cells (hESCs) and formaldehyde-fixed HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365; Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825). Immunoprecipitation Analysis: A representative lot immunoprecipitated in vitro transcribed R-loop substrate (DNA-RNA hybrid), but not doouble-stranded DNA (dsDNA) (Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825).

Kerafast抗體Anti-DNA-RNA Hybrid [S9.6] Antibody 文獻

參考文獻：

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