成人做爰免费视频免费看_成人a级高清视频在线观看,成人a大片在线观看,成人a大片高清在线观看,成人av在线播放,一a一级片,一级黄 中国色 片,一级黄 色蝶 片,一级黄色 片生活片

靶點科技(北京)有限公司

IL-33/ST2通過CCL2信號傳導和TRPV1及TRPM8的激活推動炎癥性疼痛

時間:2025-6-2閱讀:127

中文摘要:

先天免疫是宿主防御的第一道防線,并參與疼痛的發(fā)生機制。然而,先天免疫系統(tǒng)如何與感覺神經(jīng)元相互作用以調(diào)控疼痛,目前仍知之甚少。在此,我們報道白細胞介素33(IL-33)引發(fā)痛覺過敏,這一過程需要浸潤的巨噬細胞和中性粒細胞分泌趨化因子(C-C基序)配體2(CCL2),并激活感覺神經(jīng)元中的瞬時受體電位香草酸1(TRPV1)和瞬時受體電位褪黑素8(TRPM8)通道。阻斷CCL2受體(CCR2)可減輕IL-33誘導的和弗氏佐劑(CFA)誘導的熱痛覺過敏;阻斷TRPV1和TRPM8則分別減輕IL-33誘導的機械性痛覺敏感性、熱覺敏感性以及冷痛覺異常。此外,巨噬細胞的耗竭(荷蘭Liposoma巨噬細胞清除劑)減少了IL-33誘導的疼痛、CCL2的表達及后爪皮膚中腫瘤發(fā)生抑制因子2(ST2)的抑制;抑制CCR2可防止巨噬細胞和中性粒細胞的招募。我們的發(fā)現(xiàn)揭示了一個未被認識的神經(jīng)免疫串擾:浸潤免疫細胞中的IL-33-CCL2信號與感覺神經(jīng)元中的TRPV1/TRPM8相互作用,共同促進疼痛狀態(tài)。

英文摘要:

Innate immunity is the first line of host defense and contributes to pain. However, how innate immune system interacts with sensory neurons to govern pain remains poorly understood. Here, we report that interleukin 33(IL-33) initiates pain hypersensitivity that requires chemokine (C-C motif) ligand 2 (CCL2) secretion from infiltrated macrophages and neutrophils and activation of transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential melastatin 8 (TRPM8) channels in sensory neurons. Blocking CCL2 receptor (CCR2) attenuates IL-33- induced and Complete Freund’s adjuvant (CFA)-induced thermal hyperalgesia and blocking TRPV1 and TRPM8 attenuates IL-33-induced mechanical and thermal hypersensitivity and cold allodynia respectively. Furthermore, depletion of macrophages reduces IL-33-induced pain and expression of CCL2 and suppression of tumorigenicity 2 (ST2) in hindpaw skin and inhibition of CCR2 prevents recruitment of macrophages and neutrophils. Our findings reveal an unrecognized neuroimmune crosstalk of IL-33-CCL2 signaling from infiltrated immune cells with TRPV1/TRPM8 in sensory neurons to facilitate pain states.


論文信息:

論文題目:IL-33/ST2 drives inflammatory pain via CCL2 signaling and activation of TRPV1 and TRPM8

期刊名稱:Nature Communications

時間期卷:8, Article number: 724 (2025)

在線時間:2025年5月10日

DOI:doi.org/10.1038/s42003-025-08119-3

產(chǎn)品信息:

貨號:CP-005-005

規(guī)格:5ml+5ml

品牌:Liposoma

產(chǎn)地:荷蘭

名稱:Clodronate Liposomes and Control Liposomes

辦事處:Target Technology(靶點科技)

注射方式:腹腔注射

劑量和頻率:200ul/次,每天一次,連續(xù)注射兩次

氯膦酸鹽二鈉脂質(zhì)體清除單核巨噬細胞,在疼痛模型中單核巨噬細胞功能研究,荷蘭Liposoma巨噬細胞清除劑Clodronate Liposomes見刊于Nature Communications:IL-33/ST2通過CCL2信號傳導和TRPV1及TRPM8的激活推動炎癥性疼痛

IL-33/ST2通過CCL2信號傳導和TRPV1及TRPM8的激活推動炎癥性疼痛


Liposoma巨噬細胞清除劑Clodronate Liposomes氯膦酸二鈉脂質(zhì)體的材料和方法:

Drug treatment in pain models

In the pre-treatment study, ST2 neutralizing antibody (0.5?μg/10?μL, MAB10041, R&D), CCR2 antagonist (INCB3344, 0.5?μg/10?μL, Apebio) or PBS was intraplanatarly delivered 30?min before IL-33 injection. Then mechanical PWT or thermal PWL was measured at 1, 2, and 4?h after IL-33 injection. For the post-treatment study, CFA model was set up and INCB3344 was intraplantarly injected at day 3 after CFA injection, then PWL was measured. For the macrophage depletion experiment, clondronate (200?μL, CP-005-005, Liposoma) was intraperitoneal injected once a day for two days, then IL-33 was intraplantarly injected. PWT and PWL were measured at 1, 2, and 4?h after IL-33 application.

IL-33/ST2通過CCL2信號傳導和TRPV1及TRPM8的激活推動炎癥性疼痛



會員登錄

X

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標簽:
保存成功

(空格分隔,最多3個,單個標簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實性、準確性和合法性由相關(guān)企業(yè)負責,化工儀器網(wǎng)對此不承擔任何保證責任。

溫馨提示:為規(guī)避購買風險,建議您在購買產(chǎn)品前務(wù)必確認供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話
在線留言
主站蜘蛛池模板: 精品国产三级a∨在线| 国产亚洲精品久久久ai换| 六月婷婷国产精品综合| bbbbbxxxxx精品成人av| 欧美日韩精品一区在线不卡| 色情 免费 视频在线观看| 亚洲欧美综合在线一区| 动漫美女被吸乳羞羞小说| 强行破瓜粗暴顶弄蹂躏哭喊| xxxxxbbbbb欧美性极品| 中文字幕久久久人妻人区| 97无码欧美熟妇人妻蜜桃天美| 午夜精品久久久久久久爽牛战| 和女邻居做爰伦理| 特级做A爰片毛片免费看无码| 亚洲日韩在线a视频在线观看| 人妻久久久精品99系列A片毛| 青青草原精品国产亚洲AV| 无限看在线观看| 色综合久久久久久久粉嫩| 亚洲品质自拍网站| 国产福利91精品一区| 97人妻超在线观看免费| 欧美日韩国产你懂的| 亚欧精品一区二区三区四区| 亚洲欧美日韩中文在线制服| 午夜宅宅伦电影网中文字幕| 国产欧美精品亚洲日本一区| 色999日韩欧美国产| 欧美精品一区二区三区蜜桃| av毛片久久久久| 91亚洲精品综合久久水牛影| A片太大太长太深好爽A片视频| 久久久精品久久久久三级| 神电影院午夜dy888我不卡| 手机看片欧美+日韩+国产| 国产一区精彩视频在线观看| 国产亚洲精品久久久999密臂| 久久国产人妻一区二区免费| 午夜理论电影在线观看亚洲| 日本无码蜜桃波多野结衣|