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NMT作為生命科學(xué)底層核心技術(shù)，是建立活體創(chuàng)新科研平臺(tái)的*技術(shù)。2005年~2020年，NMT已扎根中國(guó)15年。2020年，中國(guó)NMT銷(xiāo)往瑞士蘇黎世大學(xué)，正式打開(kāi)歐洲市場(chǎng)。

研究使用平臺(tái)：NMT重金屬創(chuàng)新科研平臺(tái)

期刊：Journal of Hazardous Materials

主題：NMT為硅處理提升藻類(lèi)耐Cd能力提供直接證據(jù)

標(biāo)題：The roles of silicon in combating cadmium challenge in the Marine diatom Phaeodactylum tricornutum

影響因子：7.65

檢測(cè)指標(biāo)：Cd2+流速

檢測(cè)樣品：硅藻

Cd2+流實(shí)驗(yàn)處理方法：

硅藻細(xì)胞分別在低硅（17.2μM），中硅（51.7μM），高硅（86.2μM）培養(yǎng)

Cd2+流實(shí)驗(yàn)測(cè)試液成份：

8.9μM CdCl2，0.1mM KCl, 0.1mM MgCl2，0.5mM NaCl, 0.3mM MES, 0.2mM Na2SO4,  0.1% sucrose，pH=8

作者：深圳大學(xué)潘科、馬捷

中文摘要（谷歌機(jī)翻）

海洋浮游植物擁有復(fù)雜的體內(nèi)平衡網(wǎng)絡(luò)以抵消金屬毒性。環(huán)境條件的變化（例如環(huán)境營(yíng)養(yǎng)物濃度）會(huì)顯著影響其固有的金屬敏感性。

在這項(xiàng)研究中，我們?cè)u(píng)估了硅（Si）在抵消海洋硅藻Phaeodactylum tricornutum中鎘（Cd）毒性中的作用。我們首先證明了Si富集顯著提高了Cd耐受性并改變了硅藻中Cd的積累。

我們的模型表明，富硅細(xì)胞比缺硅細(xì)胞吸收更多的鎘，但鎘去除率更高。通過(guò)原子力顯微鏡和X射線(xiàn)光電子能譜檢查發(fā)現(xiàn)，富硅細(xì)胞具有更好的硅化作用，并且細(xì)胞壁中的SiO^-含量更高，這顯著降低了硅藻細(xì)胞的表面電勢(shì)并使它們吸收更多的Cd。

盡管富含Si的細(xì)胞在面對(duì)Cd脅迫時(shí)傾向于具有高的Cd負(fù)擔(dān)，但是它們通過(guò)下調(diào)流入轉(zhuǎn)運(yùn)蛋白ZIP和上調(diào)流出轉(zhuǎn)運(yùn)蛋白ATPase5-1B來(lái)抑制細(xì)胞內(nèi)Cd的增加。

我們的研究表明，硅在攜帶金屬穩(wěn)態(tài)和對(duì)抗海洋硅藻中的Cd挑戰(zhàn)中起著重要作用。

Fig. 3. Cd²⁺ fluxes around the cell surface of the diatom P. tricornutum in the testing medium containing 8.9 μM Cd²⁺. (A) Instant net Cd²⁺ fluxes measured by a non-invasive microelectrode probe; (B) mean Cd²⁺ fluxes for the L-, M-, and H-Si cells. Means do not significantly differ (p>0.05) if they bear the same letter (n = 6). L, low; M, mid; H, high.

 英文摘要

Marine phytoplankton possess a sophisticated homeostatic network to counteract metal toxicity. Changes in environmental conditions such as ambient nutrient concentrations can significantly impact their intrinsic metal sensitivity.
In this study, we evaluated the role of silicon (Si) in counteracting cadmium (Cd) toxicity in the marine diatom Phaeodactylum tricornutum. We first demonstrated that Si enrichment dramatically enhanced Cd tolerance and changed the Cd accumulation in the diatom.

Our modeling suggested that Si-enriched cells adsorbed more Cd but had a higher Cd elimination rate than the Si-starved cells. Examinations by atomic force microscopy and X-ray photoelectron spectroscopy revealed that the Si-enriched cells had better silification andmore SiO^- in the cell walls, which markedly lowered the surface potential of the diatom cells and allowed them to attract more Cd.

Although the Si-enriched cells tended to have a high Cd burden when facing Cd stress, they suppressed the increase of intracellular Cd by both down-regulating the influx transporter ZIP and up-regulating the efflux transporter ATPase5-1B.

Our study shows the significant roles Si plays in maintaining metal homeostasisand combating Cd challenge in marine diatoms.