您好, 歡迎來到化工儀器網
Environ Pollut：NMT驗證氧化石墨烯的植物毒性機制
NMT作為生命科學底層核心技術，是建立活體創新科研平臺的*技術。2005年~2020年，NMT已扎根中國15年。2020年，中國NMT銷往瑞士蘇黎世大學，正式打開歐洲市場。
研究使用平臺：NMT植物營養創新科研平臺
期刊：Environmental Pollution
主題：NMT驗證石墨烯基材料的植物毒性與其抑制硝酸鹽吸收相關
標題：Graphene oxide exposure suppresses nitrate uptake by roots of wheat seedlings
影響因子：5.714
檢測指標：NO3-流速
檢測樣品：小麥
NO3-流實驗處理方法：
培養3天的小麥苗在0/400mgL-1氧化石墨烯處理3天
NO3-流實驗測試液成份：
6mM KNO3、2mM CaNO3、0.1μM MgSO4、0.1 mM NH4H2PO4
作者：浙江工商大學都韶婷、翁軼能
中文摘要（谷歌機翻）
盡管有大量研究報告了石墨烯基材料的植物毒性，但這些材料對植物養分吸收的影響仍不清楚。
本研究表明，以200-800 mg L-1的氧化石墨烯（GO）處理的小麥植株的根部硝酸鹽濃度顯著降低。無創微電極測量結果表明，GO可以顯著抑制小麥根分生，伸長和成熟區的凈NO3-流入。
進一步的分析表明GO可能被困在根的液泡中，并且大根長度和側根數量明顯減少。另外，觀察到根尖變白，起皺，氧化應激和呼吸減弱。這些觀察結果表明GO非常不利于旺盛的根部生長，并且抑制了根部吸收面積的增加。
在分子水平上，GO暴露導致DNA損傷并抑制小麥根中大多數硝酸鹽轉運蛋白（NRT）的表達，其中顯著下調的基因是NRT1.3，NRT1.5，NRT2.1，NRT2.3和NRT2 .4。
我們得出的結論是，GO暴露會降低根系吸收面積和根系活性，并降低NRT的表達，這可能因此抑制了NO3-吸收率，導致逆境植物中硝酸鹽的不利積累。
Effect of GO on the net NO3- flux in wheat roots. GO-precultured seedlings were transferred to the microelectrode system for net NO3- flow rate measurement. (A) meristematic; (B) elongation; and (C) maturation zones. Negative flux values indicate a net influx (One-way ANOVA, n = 3).
英文摘要
Despite the large number of studies reporting the phytotoxicity of graphene-based materials, the effects of these materials on nutrient uptake in plants remain unclear.
The present study showed that nitrate concentrations were significantly decreased in the roots of wheat plants treated with graphene oxide (GO) at 200-800 mg L-1. Non-invasive microelectrode measurement demonstrated that GO could significantly inhibit the net NO3- influx in the meristematic, elongation, and mature zones of wheat roots.
Further analysis indicated that GO could be trapped in the root vacuoles, and that the maximal root length and the number of lateral roots were significantly reduced. Additionally, root tip whitening, creases, oxidative stress, and weakened respiration were observed. These observations indicate that GO is highly unfavorable for vigorous root growth and inhibits increase in root uptake area.
At the molecular level, GO exposure caused DNA damage and inhibited the expression of most nitrate transporters (NRTs) in wheat roots, with the most significantly downregulated genes being NRT1.3, NRT1.5, NRT2.1, NRT2.3, and NRT2.4.
We concluded that GO exposure decreased the root uptake area and root activity, and decreased the expression of NRTs, which may have consequently suppressed the NO3-uptake rate, leading to adverse nitrate accumulation in stressed plants.