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NMT歷*的今天丨中農學者：非損傷NMT設備檢測K+流的穩定性
??NMT作為生命科學底層核心技術，是建立活體創新科研平臺的*技術。2005年~2020年，NMT已扎根中國15年。2020年，中國NMT銷往瑞士蘇黎世大學，正式打開歐洲市場
NMT歷*的今天
2016年05月13日，中國農業大學王忠義用NMT發表了標題為The calibration model in potassium ion flux non-invasive measurement of plants in vivo in situ的研究成果。
期刊：Information Processing in Agriculture
主題：非損傷NMT設備檢測K+流的穩定性
標題：The calibration model in potassium ion flux non-invasive measurement of plants in vivo in situ
檢測指標：K+流速
作者：中國農業大學王忠義
英文摘要
NMT (Non-invasive Micro-test Technology) provides a novel electrophysiological tool which can non-invasively measure the dynamic influxes and effluxes of ions caused by the diffusion along the concentration gradients in vivo.
However, in this technique ion fluxes are converted to voltage signals using an ion selective microelectrode at a small amplitude of μV, which is easy to be interfered by the ambient noise. Hence, effective solutions to the suppression of noise and calibration of ion flux measurement system are very important for this method. A K+-selective microelectrode was constructed using liquid ion exchangers (LIX) to investigate ion transport over plant tissue.
A standard concentration gradient which simulates plant living cells was produced by an electrode with a certain tip diameter, filled with a solution containing a known K+ concentration in 100 mmol/L. An ion diffusion simulation model was established. This model evaluated the performance of ion flux measurement system in accuracy and reliability by comparing the consistency of the measured value and the predicted curve. K+ fluxes were measured within 25 minutes at each measuring point of distance 10, 20, 30, 40, 50, 80, and 100 μm from the K+ source, respectively.
It can be seen that the K+ fluxes changes little, which indicates that ion fluxes measurement system has a reliable stability. The study provides a theoretical basis for a new non-invasive ion flux measurement method creation and a new sensors design.
中文摘要（谷歌機翻）
NMT（非損傷微測技術）提供了一種新型的電生理工具，可以無創地測量由于體內濃度梯度的擴散所引起的離子的動態流入和流出。
然而，在該技術中，使用離子選擇微電極以小幅度的μV將離子通量轉換為電壓信號，這容易受到環境噪聲的干擾。因此，有效的解決方案包括抑制噪聲和校準離子通量測量系統。使用液體離子交換劑（LIX）構建K+選擇性微電極，以研究離子在植物組織上的傳輸。
由具有一定直徑的電極產生的模擬植物活細胞的標準濃度梯度，該電極填充有含有100 mmol / L已知K+濃度的溶液。建立了離子擴散模擬模型。該模型通過比較測量值和預測曲線的一致性來評估離子通量測量系統在準確性和可靠性方面的性能。在25分鐘內分別在距K +源10、20、30、40、50、80和100μm的每個測量點測量K+通量。
可以看出，K+通量變化很小，表明離子通量測量系統具有可靠的穩定性。該研究為新的非侵入式離子通量測量方法的創建和新的傳感器設計提供了理論基礎。
Fig. 8. K+ flux measured at each known position (vibration amplitude Δx is 10