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林木耐旱創新科研平臺： 轉錄因子調控楊樹根系生長影響抗旱
NMT作為生命科學底層核心技術，是建立活體創新科研平臺的*技術。2005年~2020年，NMT已扎根中國15年。2020年，中國NMT銷往瑞士蘇黎世大學，正式打開歐洲市場。
研究使用平臺：NMT林木耐旱創新科研平臺
期刊：New Phytologist
主題：轉錄因子PdNF-YB21調控楊樹根系生長并影響抗旱性
標題：The root-specific NF-Y family transcription factor, PdNF-YB21, positively regulates root growth and drought resistance by ABA-mediated IAA transport in Populus
影響因子：7.299
檢測指標：H+
檢測樣品：楊樹根
H+流實驗處理方法：
楊樹莖段在Hoagland營養液/Hoagland營養液+0.1μM ABA/Hoagland營養液+10μM FLU培養30天后檢測根。
H+流實驗測試液成份：未寫明
作者：北京林業大學夏新莉、周揚顏
中文摘要（谷歌機翻）
根系生長控制在植物對干旱脅迫的適應中起著重要作用，但是這種控制的潛在分子機制仍然很難捉摸。
在此，從胡楊中分離出根特異性核因子Y（NF-Y）轉錄因子PdNF-YB21。PdNF-YB21的功能機制通過各種形態，生理，分子，生化和光譜學技術進行了表征。
楊樹中PdNF-YB21的過表達促進木質部血管高度木質化和擴大的根系生長，從而增加了抗旱性。相反，CRISPR / Cas9介導的楊樹突變體nf-yb21表現出降低的根系生長和抗旱性。PdNF-YB21與B3域轉錄因子PdFUSCA3（PdFUS3）相互作用。PdFUS3直接激活ABA合成關鍵基因PdNCED3的啟動子，導致水分缺乏的楊樹根ABA含量顯著增加。楊NF-YB21和FUS3的共表達顯著增強了PdNCED3的表達。
此外，ABA促進了IAA在根尖的轉運，終增加了根的生長和抗旱性。
英文摘要
Root growth control plays an important role in plant adaptation to drought stress, but the underlying molecular mechanisms of this control remain largely elusive.
Here, a root-specific nuclear factor Y (NF-Y) transcription factor PdNF-YB21 was isolated from Populus. The functional mechanism of PdNF-YB21 was characterized by various morphological, physiological, molecular, biochemical, and spectroscopy techniques.
Overexpression of PdNF-YB21 in poplar promoted root growth with highly lignified and enlarged xylem vessels, resulting in increased drought resistance. In contrast, CRISPR/Cas9-mediated poplar mutant nf-yb21 exhibited reduced root growth and drought resistance. PdNF-YB21 interacted with PdFUSCA3 (PdFUS3), a B3 domain transcription factor. PdFUS3 directly activated the promoter of the ABA synthesis key gene PdNCED3, resulting in a significant increase in root ABA content in poplars subjected to water deficit. Coexpression of poplar NF-YB21 and FUS3 significantly enhanced the expression of PdNCED3.
Furthermore, ABA promoted IAA transport in root tips, which ultimately increased root growth and drought resistance.