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NMT歷*的今天丨水稻如何促進鎘吸收的文章發表
NMT歷*的今天
2015年10月14日，浙江理工大學熊杰、中國水稻所陶龍興利用NMT在Environmental and Experimental Botany上發表了標題為Excessive nitrate enhances cadmium (Cd) uptake by up-regulating the expression of OsIRT1 in rice (Oryza sativa)的研究成果。
期刊：Environmental and Experimental Botany
主題：過量的硝酸鹽通過上調水稻（Oryza sativa）中OsIRT1的表達來提高鎘（Cd）的吸收
標題：Excessive nitrate enhances cadmium (Cd) uptake by up-regulating the expression of OsIRT1 in rice (Oryza sativa)
影響因子：3.712
檢測指標：Cd2+流速
檢測部位：水稻根部
Cd2+流實驗處理方法：
三周齡的水稻幼苗，在不同的NO3濃度（0mM、2.86mM、5.72mM）下培養7天，30 mM Cd2+處理30-60min
Cd2+流實驗測試液成份：
30 μM CdCl2, 0.1 mM KCl, 0.05 mM MgCl2, 0.5 mM NaCl, 0.1 mM Na2SO4, 0.3 mM MES, pH 5.5
作者：浙江理工大學熊杰、中國水稻所陶龍興
英文摘要
In order to pursue higher yield of rice, higher amount of NO3− is often used in rice production, but limited information is available on cadmium (Cd) uptake promoted by nitrate (NO3−) in rice.
Here, hydroponic experiments with integration of three NO3− levels (0 mM, 2.86 mM and 5.72 mM simplify as 0, NO3− and 2NO3−) and two Cd concentrations (0 and 100 μM) were conducted. Phenotypes and physiological indexes related to Cd uptake were measured; content changes of nitrogen, Cd and Fe also were measured. In addition, net Cd2+ flux was monitored and quantitative RT-PCR was applied to determine the expression of Fe/Cd transporters.
The results showed that although excessive NO3− had no significant promoting effect on rice growth in absence or presence of Cd, it enhanced Cd influx in elongation zone of rice roots and increased Cd accumulation in rice plants and grains. Excessive NO3− also increased OsIRT1 expression and Fe accumulation in rice seedlings in the absence or presence of Cd.
In conclusion, excessive NO3− increases OsIRT1 expression and Cd influx in elongation zone of rice roots, and then it increased Cd and Fe uptake and accumulation in rice plants and grains, which raises an increased-risk for human health.
中文摘要（谷歌機翻）
為了追求更高的水稻產量，通常在水稻生產中使用大量的NO3-，但是關于水稻中硝酸鹽（NO3-）促進鎘（Cd）吸收的信息有限。
在這里，進行了整合三個NO3-濃度（0 mM，2.86 mM和5.72 mM簡化為0，NO3-和2NO3-）和兩個Cd濃度（0和100μM）的水培實驗。測量與鎘吸收有關的表型和生理指標；還測量了氮，鎘和鐵的含量變化。此外，監測凈Cd2 +通量并應用定量RT-PCR確定Fe / Cd轉運蛋白的表達。
結果表明，盡管過量的NO3-在沒有或沒有Cd的情況下對水稻的生長沒有明顯的促進作用，但是它增加了Cd在水稻根部伸長區的流入量，并增加了Cd在水稻和谷物中的積累。在沒有或沒有鎘的情況下，過量的NO3-也會增加水稻幼苗中OsIRT1的表達和鐵的積累。
總之，過量的NO3-會增加稻根伸長區OsIRT1的表達和Cd的流入，然后增加稻米和谷物中Cd和Fe的吸收和積累，從而增加人體健康的風險。
Effects of different concentrations of nitrate (NO3??) on root net Cd2+ in?ux in rice seedlings. Root net Cd2+ in?ux of 3-week-old rice seedlings c*ted in 0, 2.86 and 5.72 mM NO3?? conditions for 7 days. Then, 30 mM Cd2+ were added into the solution for 30–60 min. Net Cd ?ux of meristematic zone (B and D) and elongation zone (A and C) of the main root were monitored and steady-state Cd ?ux was recorded for 10 min. Bar indicates 50 mm in (A and B), the values are means ?? SE (n = 107), different letters on the bar indicate signi?cant differences at P = 0.05.