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CarboPac PA200碳水化合物色譜柱
對于高分辨率測繪和糖蛋白釋放的寡糖的分析，使用的Thermo Scientific戴安CARBOPACPA200分析及保護柱提供的敏感性和特異性檢測。脈沖安培檢測（PAD）相結合，此陰離子交換柱提供無需使用衍生快速，重復分離。疏水性聚合物，薄殼型陰離子交換樹脂填充材料是在pH值0-14的范圍內保持穩定。這種*的pH值穩定性允許使用有利于碳水化合物的陽極氧化的金電極洗脫液組成的。
CarboPac PA200碳水化合物色譜柱
訂貨信息：
Dionex CarboPac PA200 碳水化合物分析柱
| 分析柱 | |
| CarboPac PA200 Analytical Column (3 x 250 mm) | 062896 |
| 保護柱 | |
| CarboPac PA200 Guard Column (3 x 50 mm) | 062895 |
Fast, pH-Stable Separation of Oligosaccharides
A variety of HPLC approaches are proposed for characterizing oligosaccharides, but many prove inadequate for separating complex mixtures and are limited by a lack of specificity and high limits of detection. Dionex has developed an improved HPLC technique for carbohydrate analysis based on anion-exchange chromatography coupled with pulsed amperometric detection. This technique provides high-resolution separations with sensitive and specific detection, without derivatization.
Sensitive, direct detection with no derivatization, using pulsed amperometric detection
Very high-efficiency separations
Separations based on size, charge, degree of branching, and linkage isomerism
Low flow rate saves on eluent consumption
Does not require derivatization
CarboPac? PA200 columns are packed with a hydrophobic, polymeric, pellicular anion-exchange resin stable over the range of pH 0–14. The unique pH-stability of the packing material allows the use of eluent compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes.
| CarboPac PA200 Column for High-Resolution Oligosaccharide Separations Specifications | |
|---|---|
| Dimensions | CarboPac? PA200 Analytical Column: 3 × 250 mm CarboPac PA200 Guard Column: 3 × 50 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
| Temperature range | 6–60 °C Recommended operating temperature: controlled ambient |
| Typical operating conditions | 2700 psi at 0.5 mL/min (analytical and guard columns) Recommended flow rate: 0.5 mL/min |
| Mobile phase compatibility | pH 0–14; 100% compatible with common organic solvents. Ionic form eluents: sodium acetate and sodium hydroxide only. |
| Resin composition | 5.5 μm-diameter ethylvinylbenzene/divinylbenzene substrate (55% cross-linked) agglomerated with 43 nm MicroBead? quaternary amine functionalized latex (6% crosslinked) |
| Anion-exchange capacity | Approximately 35 μeq/column (3 × 250 mm analytical column) |
| Column construction | PEEK? with 10-32 threaded ferrule-style end fittings. All components are nonmetallic |
