9
當前位置：> 供求商機> 350301-5ML——胺基活化瓊脂糖凝膠6HF
Product Introduction:Amine-activated agarose beads can be used for the immobilization of carboxyl containing or aldehyde containing molecules. The coupling chemistry is carbodiimide-based and is a successful and well-documented technique. Amine-activated SepFast forms a chemically stable amide linkage with ligands containing carboxyl groups. It has a long spacer (9 atoms). The pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography adsorbents for both small scale and large scale purification applications.
Product Properties:
Amine-activated SepFast 4 High Flow (4HF) is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules.
The base matrix is activated by attaching a long hydrophilic chain with an amine group in the end. They react with either carboxyl containing ligands in the presence of a carbodiimide agent, or aldehyde containing ligands in the presence of a reducing agent. Amine-activated SepFast is supplied as an aqueous suspension in 20% ethanol.
Group to be coupled | -COOH, CHO |
Matrix | Highly cross-linked 4% agarose beads (4HF) |
Particle size | 50 – 150 µm |
Particle size | 50 – 150 µm |
Spacer arm | 9 atoms |
Activation level | 10 - 25 µmol amine / ml medium |
pH stability | 2 -13 (ligand dependent) |
Chemical stability | Compatible with all commonly used aqueous chemicals, provided the ligand to be coupled can withstand |
Storage | +4℃—+8℃ |
Product Series:
Product | Quantity | Code no. |
Amine-activated SepFast 4HF | 5 ml | 350101-5ML |
50 ml | 350101-50ML | |
1 Litre | 350101-1L | |
Amine-activated SepFast 6HF | 5 ml | 350301-5ML |
50 ml | 350301-50ML | |
1 Litre | 350301-1L |
請輸入賬號
請輸入密碼
請輸驗證碼
以上信息由企業自行提供，信息內容的真實性、準確性和合法性由相關企業負責，化工儀器網對此不承擔任何保證責任。
溫馨提示：為規避購買風險，建議您在購買產品前務必確認供應商資質及產品質量。
