成人做爰免费视频免费看_成人a级高清视频在线观看,成人a大片在线观看,成人a大片高清在线观看,成人av在线播放,一a一级片,一级黄 中国色 片,一级黄 色蝶 片,一级黄色 片生活片

深圳欣博盛生物科技有限公司
中級會員 | 第5年

13129540117

Epicypher SNAP-ChIP® K-MetStat Panel

時間:2023/11/8閱讀:495
分享:

由147個堿基對條形碼widom601定位序列DNA纏繞在由大腸桿菌表達的重組人組蛋白組裝而成的一組明顯修飾的單核小體(組蛋白H2A、H2B、H3和H4各2個;accession numbers:H2A-P04908、H2B-O60814、H3.1-P68431或H3.2-Q71DI3*、H4-P62805),由1個未修飾的加上15個組蛋白H3或H4翻譯后修飾(PTMs,通過專有的半合成方法創建)組成:H3K4、K9、K27和H4K20與me1、me2或me3。每個明顯修飾的核小體都可以通過3'端的獨-特DNA序列(“barcode")進行區分,該序列可以通過qPCR或二代測序進行破譯。池中的16個核小體都被2種不同物種的DNA纏繞,每一種都含有獨-特的條形碼(“A"和“B",參考SNAP-ChIP手冊)。適合用作ChIP、抗體特異性測試或效應蛋白結合實驗的摻入質控品。

*組蛋白H3.2在110位包含一個Cys到Ala的替換。


產品詳情

保存溫度: Stable for six months at -20°C from date of receipt.

運輸溫度: DO NOT FREEZE!! Frozen cold packs.

產品形式: Purified recombinant mononucleosomes, containing a mixture of 16 (1 unmodified plus 15 unique) H3 and H4 PTMs in 10 mM sodium cacodylate pH 7.5, 100 mM NaCl, 1 mM EDTA, 50% glycerol (w/v), 1x Protease Inhibitor cocktail, 100 µg/mL BSA, 10 mM β-mercaptoethanol. Average molarity = 0.6 nM. MW = ~199382.1 Da (average MW of all 16 nucleosomes).


Epicypher熱銷產品——SNAP-ChIP® K-MetStat Panel


驗證數據

Fig 1. DNA Gel Data: Representative images for SNAP-ChIP K-MetStats (H3K4me0 = unmodified, H3K4me2, H3K4me3) run on a native PAGE gel and stained with ethidium bromide to visualize DNA. Lane 1: Free 147bp DNA used in nuclesome assembly (100 ng). Lane 2: Intact nucleosomes (200 ng) showing lack of free DNA. Identical experiments were performed for the entire K-MetStat Panel.

Epicypher熱銷產品——SNAP-ChIP® K-MetStat Panel


Fig 2. Protein Gel Data: Representative Coomassie stained PAGE gel for SNAP-ChIP K-MetStats (2 µg each of unmodified, H3K4me1, H3K4me2, H3K4me3) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated. Identical experiments were performed for the remainder of the K-MetStat Panel.

Epicypher熱銷產品——SNAP-ChIP® K-MetStat Panel


Fig 3. ChIP Data: Representative images for SNAP-ChIP K-MetStats (unmodified, H3K4me1, H3K4me2, H3K4me3) assayed in a chromatin immunoprecipitation (ChIP) experiment using commercially available ChIP grade antibodies (3 µg, n = 3). Quantitative Real-Time PCR (qPCR) for the DNA barcodes corresponding to unmodified (H3K4me0), H3K4me1, H3K4me2, and H3K4me3 nucleosomes show recovery of the barcodes corresponding to the expected antibody target. Identical experiments were performed for the remainder of the K-MetStat Panel (H3K9me1, H3K9me2, H3K9me3, H3K27me1, H3K27me2, H3K27me3, H3K36me1, H3K36me2, H3K36me3, H4K20me1, H4K20me2 and H4K20me3).

Epicypher熱銷產品——SNAP-ChIP® K-MetStat Panel

Fig 4. ChIP Data: Representative chromatin immunoprecipitation (ChIP) data using commercially available ChIP-grade antibodies targeting each PTM  in the K-MetStat panel.  The antibodies were assayed in a native ChIP experiment with 3 μg antibody added to 3 μg K-562 cell chromatin with  the K-MetStat Panel spiked-in prior to micrococcal nuclease digestion.  Quantitative real-time PCR (qPCR) was used to measure recovery of  duplicate DNA barcodes corresponding to the indicated panel nucleosomes (blue bars, x-axis).  The black bars map to the log scale on the right  y-axis and indicate the percentage of target immunoprecipitated relative to the input (a measure of the antibody efficiency).  In each case, the SNAP-ChIP spike-in confirmed that the antibodies recovered the expected histone PTM.  Enrichment of off-target PTMs is due to antibody  cross-reactivity.


訂購詳情

貨號

產品名稱

規格

19-1100       

SNAP-ChIP® K-MetStat Panel               

200 µL        





Epicypher熱銷產品——CUTANA™ pAG-Tn5 for CUT&Tag

如需了解更多詳細信息或相關產品,請聯系EpiCypher中國授權代理商-欣博盛生物 

會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標簽:
保存成功

(空格分隔,最多3個,單個標簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復您~
撥打電話
在線留言
主站蜘蛛池模板: 九九热精品在线| 91视频一区二区| 国产精品久久久久久3d| 国产精品久久久久久夜夜夜| 国产精品大屁股白浆视频| 欧美一区二区三区不卡| 高H喷水荡肉爽腐男男软件| 亚洲精品7久久久久久久久| 精品亚洲一区二区在线播放| 洲精品无码高潮喷水A片| 久久99国产综合精品免费| 小说H高H激情早餐C| 日本一本二本无码免费视频| 日韩精品无码一区AAA片| 国产精品久久久久久久久久免费| 老湿机费x一分钟影院体验区| 久久国产精品亚洲欧美阿娇| 国产精品久久久久久久久软件| 越南女子杂交内射BBWXZ| 出差被公舔到高潮| 欧美久久精品免费看C片| 免费一级毛片在线视频观看| 国产偷抇久久精品A片69探花| 福利在线免费观看91| 中文字幕亚洲欧美日韩专区| 国产乱国产乱老熟300部视频| 黑料门独家爆料吃瓜在线| 神马影院手机在线线| 久久精品国产亚洲av久试看| 国产欧美日韩综合精品| A欧美爰片久久毛片A片| 久久亚洲春中文字幕久久久| 日韩国产在线观看一区| 国产一区二区精品久久岳| 国产在线拍小情侣国产拍拍偷| 欧美日韩在线人妻| 欧美天天综合网| 久久久无码人妻精品无码| 国产一区二区三区精品推荐| 欧美一区日韩二区国产三区| 亚洲AV福利天堂一区二区三|