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深圳欣博盛生物科技有限公司
中級會員 | 第5年

13129540117

Epicypher:BRD4 CUTANA™ CUT&RUN Antibody

時間:2023/10/17閱讀:464
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EpiCypher是一家為表觀遺傳學和染色質生物學研究提供高質量試劑和工具的專業制造商。EpiCypher推出的CUT&RUN級別的BRD4 Antibody符合EpiCypher的“CUTANA Compatible"標準,可用于核酸酶靶向切割和釋放(CUT&RUN)和/或靶向剪切及轉座酶技術(CUT&Tag)的基因組定位。BRD4抗體主要在轉錄起始位點的側翼產生CUT&RUN峰(Fig. 1)。正如文獻所報道,BRD4峰與BRG1/SMARCA4峰有很大程度的重疊(Fig. 2)。


產品詳情

反應種屬: Human, Mouse

宿主來源: Rabbit

實驗應用: CUT&RUN, IHC, IP, WB

免疫原: Between amino acids 1312 and 1362

克隆性: Polyclonal

保存溫度: Stable for 1 year at 4°C from date of receipt

運輸溫度: DO NOT FREEZE!! Frozen cold packs.

產品形式: Antigen affinity-purified antibody in Tris-citrate/phosphate buffer pH 7-8, 0.09% sodium azide


驗證數據

Epicypher熱銷產品——BRD4 CUTANA™ CUT&RUN Antibody


Figure 1: BRD4 peaks in CUT&RUN

CUT&RUN was performed as described above. Peaks were called with MACS2. Heatmaps show BRD4 peaks relative to IgG negative control antibody and H3K4me3 positive control antibody in aligned rows ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal. All rows were aligned relative to H3K4me3 antibody.

Epicypher熱銷產品——BRD4 CUTANA™ CUT&RUN Antibody



Figure 2: BRD4 CUT&RUN representative browser tracks

CUT&RUN was performed as described above. Gene browser shots were generated using the Integrative Genomics Viewer (IGV, Broad Institute). Three representative loci show overlap of BRD4 and BRG1 peaks.

Epicypher熱銷產品——BRD4 CUTANA™ CUT&RUN Antibody

Figure 3: Immunohistochemistry data

FFPE sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) using BRD4 antibody at a dilution of 1:5,000.



Epicypher熱銷產品——BRD4 CUTANA™ CUT&RUN Antibody


Figure 4: Immunoprecipitation data

EpiCypher BRD4 antibody (6 µg) was used to immunoprecipitate whole cell lysates (1 mg, 20% of IP loaded) isolated from HeLa cells. A negative control IgG antibody and positive control antibody targeting BRD4 (Bethyl Laboratories) were also used to demonstrate the specificity of the IP. For blotting immunoprecipitates, Bethyl Laboratories BRD4 antibody (A700-004) was used at a dilution of 1:1,000.


訂購詳情

貨號

產品名稱

規格

13-2003

BRD4 CUTANA™ CUT&RUN Antibody

50 µL



Epicypher熱銷產品——CUTANA™ pAG-Tn5 for CUT&Tag

如需了解更多詳細信息或相關產品,請聯系EpiCypher中國授權代理商-欣博盛生物


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