炎癥小體（inflammasome）的研究

時(shí)間：2023/1/17閱讀：919

炎癥小體（inflammasome），是由胞漿內(nèi)模式識(shí)別受體(PRRs)參與組裝的多蛋白復(fù)合物,是天然免疫系統(tǒng)的重要組成部分。炎癥小體能夠識(shí)別病原相關(guān)分子模式(PAMPs)或者宿主來(lái)源的損傷相關(guān)分子模式(DAMPs),募集和激活半胱天冬酶-1（Caspase-1）。活化的Caspase-1可切割I(lǐng)L-1β和IL-18前體蛋白,使其產(chǎn)生成熟的細(xì)胞因子。炎癥小體的活化還能夠誘導(dǎo)細(xì)胞的炎癥壞死(pyroptosis)。

目前已發(fā)現(xiàn)的炎癥小體主要由NLRP1炎癥小體、NLRP3炎癥小體、IPAF(NLRC4)炎癥小體、NLRP6炎癥小體、RIG-I炎癥小體和AIM2炎癥小體。其中NLRP3炎癥小體作為天然免疫的重要組分在多種疾病過(guò)程中都發(fā)揮了關(guān)鍵作用,包括家族性周期性自身炎癥反應(yīng)、2型糖尿病、阿爾海默茨病和動(dòng)脈粥樣硬化癥等。因此,作為炎癥反應(yīng)的核心,NLRP3炎癥小體可能為各種炎癥性疾病的治療提供新的靶點(diǎn) 。

炎癥小體誘導(dǎo)劑
產(chǎn)品名稱(chēng)	貨號(hào)	描述	規(guī)格	品牌
NLRP3炎癥小體誘導(dǎo)劑
Alum Crystals	tlrl-alk	Aluminium potassium sulfate	1g	invivogen
ATP	tlrl-atp	Adenosine 5'-triphosphate disodium salt	1g	invivogen
CPPD Crystals	tlrl-cppd	Calcium Pyrophosphate Dihydrate crystals	5mg	invivogen
Hemozoin	tlrl-hz	Synthetic heme crystal	5mg	invivogen
MSU Crystals	tlrl-msu	Monosodium Urate Crystals	5mg	invivogen
Nano-SiO2	tlrl-sio	Nanoparticles of silica dioxide	10mg	invivogen
Nigericin	tlrl-nig	Nigericin, sodium salt	10/50mg	invivogen
AIM2炎癥小體誘導(dǎo)劑
Poly(dA:dT)	tlrl-patn	RIG-I Agonist - CDS Agonist	200ug/1mg	invivogen

炎癥小體抑制劑
產(chǎn)品名稱(chēng)	貨號(hào)	產(chǎn)品描述	規(guī)格	品牌
Bay11-7082	tlrl-b82	NLRP3 Inflammasome Inhibitor - IκB-α Inhibitor	10mg	invivogen
Glybenclamide	tlrl-gly	NLRP3 Inflammasome Inhibitor - Proton pump inhibitor	1g	invivogen
Z-VAD-FMK	tlrl-vad	NLRP3 Inflammasome Inhibitor - Caspase inhibitor	1mg	invivogen

Figure: Mouse caspase-1 is detected in cell culture supernatants by ELISA using caspase-1 (mouse) Matched Pair Detection Set (Prod. No AG-46B-0003).

Adipogen公司也可提供多種炎癥小體信號(hào)相關(guān)的研究試劑
定量檢測(cè)炎癥小體的活化
產(chǎn)品名稱(chēng)：Caspase-1 (mouse) Matched Pair Detection Set
產(chǎn)品編號(hào)：AG-46B-0003-KI01 規(guī)格：1 Set
特異性： Detects mouse caspase-1 (p10 and p20 domain).
Does not detect human caspase-1.
反應(yīng) 性： Mouse
靈敏度： 100pg/ml
檢測(cè)范圍： 0.15ng/ml to 10ng/ml
樣本類(lèi)型： Cell Culture Supernatant

Figure: Mouse caspase-1 (p10) is detected by immunoblotting using anti-Caspase-1 (p10) (mouse), mAb (Casper-2) (Prod. No AG-20B-0044).

用于檢測(cè)活化的小鼠Caspase-1(p10&p20)的特異性單抗
產(chǎn)品名稱(chēng)：anti-Caspase-1 (p10) (mouse), mAb (Casper-2)
產(chǎn)品編號(hào)： AG-20B-0044-C100 100 μg，AG-20B-0044B-C100 Biotin 100 μg
克隆號(hào)： Casper-2
亞型： Mouse IgG2a
免疫原： Recombinant mouse caspase-1
應(yīng)用： WB (1μg/ml)
特異性： Recognizes endogenous full-length and activated (p10 fragment) mouse caspase-1.

產(chǎn)品名稱(chēng)	產(chǎn)品編號(hào)	克隆號(hào)	亞型	免疫原	應(yīng)用	特異性
anti-Caspase-1 (p20) (mouse), mAb (Casper-1)	AG-20B-0042-C100 100 μg， AG-20B-0042B-C100 Biotin 100 μg	Casper-1	Mouse IgG1	Recombinant mouse caspase-1	WB (1μg/ml) (see online protocol), IHC (PS), IP	Recognizes endogenous full-length and activated (p20 fragment) mouse caspase-1.

產(chǎn)品名稱(chēng)	產(chǎn)品編號(hào)	克隆號(hào)	亞型	免疫原	應(yīng)用	特異性
anti-Caspase-1 (p20) (human), mAb (Bally-1)	AG-20B-0048-C100 100 μg	Bally-1	Mouse IgG1	Recombinant human caspase-1	WB (1μg/ml) (see online protocol)	Recognizes endogenous full-length and activated (p20 fragment) human caspase-1.

標(biāo)準(zhǔn)炎癥小體信號(hào)抗體（standard inflammasome signaling antibodies）
產(chǎn)品名稱(chēng)	產(chǎn)品編號(hào)	規(guī)格	品牌
anti-Asc, pAb (AL177)	AG-25B-0006-C100	100 μg	AdipoGen
anti-AIM2 (human), mAb (3B10)	AG-20B-0040-C100	100 μg	AdipoGen
anti-Cardif (human), mAb (Adri-1)	AG-20B-0004-C100	100 μg	AdipoGen
anti-MDA5 (human), mAb (Hely-1)	AG-20B-0013-C100	100 μg	AdipoGen
anti-Nod1 (human), pAb (AL184)	AG-25B-0013-C050	50 μg	AdipoGen
anti-NS3 (HCV), mAb (1B6)	AG-20B-0001-C100	100 μg	AdipoGen
anti-NS5B (HCV), mAb (5B-3B1)	AG-20B-0002-C100	100 μg	AdipoGen
anti-NS5B (HCV), mAb (blocking) (5B-12B7)	AG-20B-0003-C100	100 μg	AdipoGen
anti-Pyrin (human), pAb (AL196)	AG-25B-0020-C100	100 μg	AdipoGen
anti-RIG-I, mAb (Alme-1)	AG-20B-0009-C100	100 μg	AdipoGen
anti-RIG-I, mAb (Alme-1) (Biotin)	AG-20B-0009B-C100	100 μg	AdipoGen

其他炎癥小體信號(hào)抗體（other inflammasome signaling antibodies）
產(chǎn)品名稱(chēng)	產(chǎn)品編號(hào)	規(guī)格	品牌
anti-HMGB1, mAb (rec.) (Giby-1-4)	AG-27B-0002-C100	100 μg	AdipoGen
anti-HMGB1, mAb (rec.) (Giby-1-4) (Biotin)	AG-27B-0002B-C100	100 μg	AdipoGen
anti-NAIP1/2/5 (mouse), mAb (Naipa-1)	AG-20B-0045-C100	100 μg	AdipoGen
anti-NLRP1/NALP1 (human), pAb (AL176)	AG-25B-0005-C100	100 μg	AdipoGen
anti-NLRP3/NALP3 (mouse), mAb (Cryo-1)	AG-20B-0006-C100	100 μg	AdipoGen
anti-NLRP6/NALP6 (human), mAb (Clint-1)	AG-20B-0046-C100	100 μg	AdipoGen
anti-NLRP12/NALP12 (human), pAb (AL236)	AG-25B-0021-C100	100 μg	AdipoGen

炎癥小體信號(hào)相關(guān)的蛋白&抗體
產(chǎn)品名稱(chēng)	產(chǎn)品編號(hào)	規(guī)格	品牌
IL-1β (human) (rec.) (untagged)	AG-40B-0023-C010	10 μg	AdipoGen
IL-1β (human) (rec.) (untagged) (MultiPack)	AG-40B-0023-3010	3 x 10 μg	AdipoGen
IL-1β (mouse) (rec.) (untagged)	AG-40B-0086-C010	10 μg	AdipoGen
IL-1β (mouse) (rec.) (untagged) (MultiPack)	AG-40B-0086-3010	3 x 10 μg	AdipoGen
IL-1 Receptor Type I (human):Fc (human) (rec.)	AG-40B-0024-C050	50 μg	AdipoGen
IL-1 Receptor Type I (human):Fc (human) (rec.) (MultiPack)	AG-40B-0024-3050	3 x 10 μg	AdipoGen
anti-IL-1α (mouse), mAb (Bamboo-1)	AG-20B-0050-C100	100 μg	AdipoGen
anti-IL-1R2 (mouse), mAb (rec.) (blocking) (Praxy-1)	AG-27B-0011-C100	100 μg	AdipoGen