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[供應]Cellartis Y30050 Hepatocyte Differentiation Kit 分化獲取肝細胞試劑盒
華雅旗下紅榮微再*代理（上海）TAKARA CellArtis，Rubicon產品 詳詢 1500 1904 520 紅榮微再-客服: 經銷商專員
Cellartis-Y30050 Cellartis Hepatocyte Differentiation Kit
產品英文名稱：Cellartis Hepatocyte Differentiation Kit
產品中文名稱：Cellartis分化獲取肝細胞試劑盒
用于誘導內胚層細胞（definitive endoderm (DE)）獲取肝細胞
產品概述：
The Cellartis Hepatocyte Differentiation Kit用于誘導內胚層細胞（definitive endoderm (DE)）獲取肝細胞。通過使用本產品獲取的肝細胞表現出符合預期的代謝酶表達，從而可以用于藥物代謝研究和毒理學篩選。本產品包含足夠的維持培養基用于正常為期11天的實驗。
The Cellartis Hepatocyte Differentiation Kit可以對廣泛來源的DE細胞進行定向分化：客戶自己的DE細胞，或是Cellartis提供的標準化DE細胞，或是客戶利用Cellartis Definitive Endoderm Differentiation Kit獲得的DE細胞。據估計，現在已經有28種發源于人類干細胞的DE細胞被成功測試可以使用本產品。特別地，如果客戶是用于大規模、要求持續穩定獲得肝細胞的相關用途，Cellartis推薦Definitive Endoderm Cells (from ChiPSC18)，用作起始DE細胞材料，或是用作陽性對照品。如果客戶要求的DE是來自于自有的樣本材料（patient-specific, or disease-specific），建議使用Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)。
The Cellartis Hepatocyte Differentiation Kit generates highly homogeneous hepatocyte cultures starting from definitive endoderm (DE) cells. The resulting hepatocytes show the expected expression of relevant metabolic enzymes, making them suitable for drug metabolism studies and toxicity screening. The Cellartis Hepatocyte Differentiation Kit contains sufficient maintenance medium to perform experiments in an 11-day time window.
There are several options for source materials to generate functional hepatocytes: your own DE cells, our DE cells, or DE cells you generate using the Cellartis Definitive Endoderm Differentiation Kit. This kit has been successfully tested with DE cells derived from 28 different human pluripotent cell lines. Our cells, Cellartis Definitive Endoderm Cells (from ChiPSC18), are suitable as the starting material or as a positive control. If you desire a customized (standard, patient-specific, or disease-specific) genetic background for your DE cells, use the complete Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055).
產品特點:
完整的試劑盒 - 包括四種不同的培養基和即用包被，足以進行為期23天的方案，將定形內胚層（DE）細胞分化為肝細胞
方法可靠 - 使用超過25種不同的干細胞系測試過
有效的分化 - 產生> 90％純度的肝細胞
Complete kit—includes four different media sets and a ready-to-use coating, sufficient for a 23-day protocol for differentiating definitive endoderm (DE) cells into hepatocytes
Robust protocol—tested with more than 25 different stem cell lines
Efficient differentiation—yields >90% pure hepatocytes
組份： –20°C保存
包括解凍接種培養基、祖代培養基、成熟培養基及添加劑、維持培養基和包被劑
1 bottle Hepatocyte Thawing and Seeding Medium (1) (25 ml)
1 bottle Hepatocyte Progenitor Medium (2) (50 ml)
2 bottles Hepatocyte Maturation Medium Base (3A) (24 ml)
1 tube Hepatocyte Maturation Medium Supplement (3B) (2.6 ml)
3 bottles Hepatocyte Maintenance Medium (4) (50 ml)
1 tube Hepatocyte Coating (7.5 ml)
自備試劑：
人類定形內胚層細胞（推薦Cellartis 的DE細胞）
胎牛血清（FBS）或敲除血清替代品（KO-SR）
PBS Dulbecco's不含Ca 2+和Mg 2+（D-PBS - / - ）
TrypLE選擇酶（1X），不含酚紅
產品性能：
Definitive endoderm (DE) cells were differentiated into hepatocytes using the Cellartis Hepatocyte Differentiation Kit. The first field of view, labeled “Definitive Endoderm Day 7,” shows DE cells seven days after differentiation from human pluripotent cells. These DE cells became the starting material for the differentiation kit. The remaining progression from DE cells to hepatocytes shows the expected morphology over a 14-day time period. The morphological features present in the last field of view, labeled “Hepatocyte Day 21+,” continue stably past Day 21.
人iPS誘導分化成肝細胞全過程的完整試劑盒：Y30055（新產品）
人iPS來源肝細胞株及*培養基Y10133/Y10134/Y10135（新產品）
人原代肝臟細胞培養基 Y20020（新產品）
http://www.js967.com/st321032/article_1496885.html
華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司主營細胞治療和精準醫療等產品，2018年與TAKARA繼續合作，*代理（上海）其旗下Cellartis干細胞研究制品和Rubicon單細胞文庫構建試劑。Cellartis公司位于瑞典，擁有iPS細胞等干細胞分化為肝細胞及臟器細胞的分化誘導技術和ES細胞，ips細胞，分化細胞等干細胞相關產品。
Cellartis-Y30050 Cellartis Hepatocyte Differentiation Kit 手機1500 1904 520 紅榮微再-客服 經銷商專員
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