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[供應]PeproTech 大血管內皮細胞培養基 PeproGrow 700-MacroV
PeproGrow MacroV 大血管內皮細胞培養基適用于培養大血管內皮細胞。能良好維持內皮祖細胞的形態和功能，并提高特定標志物—內皮一氧化氮合酶活性。
PeproGrow™ MacroV (Macrovascular Endothelial Cell Media)
英文名：PeproGrow™ MacroV (Macrovascular Endothelial Cell Media)
中文名：PeproGrow™ MacroV 大血管內皮細胞培養基
PeproGrow MacroV 大血管內皮細胞培養基適用于培養大血管內皮細胞。能良好維持內皮祖細胞的形態和功能，并提高特定標志物—內皮一氧化氮合酶活性。
概述
PeproGrow™ MacroV 大血管內皮細胞培養基
Product Details
Catalog Number:700-MacroV
Description:
PeproTech offers three separate endothelial cell culture media formulations developed for the in vitro c*tion of: endothelial progenitor cells (EPC; PeproGrow EPC) derived from bone marrow or peripheral blood; endothelial cells from large vessels (PeproGrow MacroV); and endothelial cells from small vessels (PeproGrow MicroV). These media formulations maintain outstanding endothelial cell morphology and function, and increase the activity of endothelial nitric oxide synthase (eNOS), which account for a specific, crucial marker for endothelial cells. By doing this, the media provide an optimal cell culture environment for macrovascular and microvascular endothelial cells, as well as for EPC; growing cells at rates that exceed commercially available media. These media formulations are optimized for primary/progenitor human cells, but can also be used for bovine, porcine, rabbit, rat, and mouse EPC, or mature endothelial cells.
PeproGrow MacroV is supplied as a 500mL bottle of basal medium and a separate, frozen growth factor component.
PeproGrow MacroV內皮細胞培養基適用細胞：
PeproGrow MacroV is recommended for Macrovascular Endothelial Cells:
Human Umbilical Vein Endothelial Cells (HUVEC) 人臍靜脈內皮細胞
Human Umbilical Artery Endothelial Cells (HUAEC) 人臍動脈內皮細胞
Human Aortic Endothelial Cells (HAoEC) 人大動脈內皮細胞
Human Pulmonary Artery Endothelial Cells (HPAEC) 人肺動脈內皮細胞
Human Saphenous Vein Endothelial Cells (HSaVEC) 人隱靜脈內皮細胞
And other large vessel (macrovascular) endothelial cells 以及其他大血管內皮細胞
PeproGrow MacroV內皮細胞培養基用于人臍靜脈內皮細胞擴增的性能
Figure 1: HUVEC Proliferation. HUVEC were seeded onto fibronectin-coated plates and incubated for 7 days in PeproGrow MacroV basal medium + Growth Supplement MacroV and two competitors' medium. The proliferation ability of HUVEC was assessed 7 days after cell c*tion using the XTT assay following the manufacturer’s protocol. The proliferation ability of HUVEC was expressed as the average of fluorescence intensity (O.D.) calculated using a plate reader from two independent assays run in triplicates.
人臍靜脈內皮細胞擴增
PeproTech 內皮細胞培養基系列
PeproTech提供三種內皮細胞培養基試劑盒，分別適用于體外培養：骨髓或外周血來源的內皮祖細胞（PeproGrow™ EPC ），大血管內皮細胞（PeproGrow™ MacroV）和微血管內皮細胞（PeproGrow™ MicroV）。每款試劑盒由基礎培養基和一管單獨的內皮細胞生長添加劑組成。
品牌 | 貨號 | 產品描述 | 包裝 |
PeproTech | 700-EPC | PeproGrow™ EPC (Endothelial Progenitor Cell Media)內皮祖細胞培養基試劑盒 | 1 Kit |
PeproTech | 700-MacroV | PeproGrow™ MacroV (Macrovascular Endothelial Cell Media)大血管內皮細胞培養基試劑盒 | 1 Kit |
PeproTech | 700-MicroV | PeproGrow™ MicroV (Microvascular Endothelial Cell Media)微血管內皮細胞培養基試劑盒 | 1 Kit |
其他內皮細胞培養基
ScienCell 1001 內皮細胞培養基 現貨供應
歡迎您致電 華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司 ：1500 1904 520。紅榮微再-客服: 2395557778 經銷商專員
背景——人臍靜脈內皮細胞
來源：Wiki
Human umbilical vein endothelial cells (HUVECs) are cells derived from the endothelium of veins from the umbilical cord. They are used as a laboratory model system for the study of the function and pathology of endothelial cells (e.g., angiogenesis).[1] They are used due to their low cost, and simple techniques for isolating them from umbilical cords, which are normally resected after childbirth. HUVECs were first isolated and cultured in vitro in the 1970s by Jaffe and others. [2] HUVECs can be easily made to proliferate in a laboratory setting. Like human umbilical artery endothelial cells they exhibit a cobblestone phenotype when lining vessel walls.
人臍靜脈內皮細胞來自臍帶血管的內皮細胞。因為價格低和分離技術簡單，人臍靜脈內皮細胞通常作為內皮細胞的功能和生理研究的實驗模型使用。人臍靜脈內皮細胞是在1970年由Jaffe等人*分離使用的。在實驗條件下人臍靜脈內皮細胞很容易擴增。
人臍靜脈內皮細胞（HUVEC）獲取
來源：互聯網
1. 將 15-20cm 長的新生兒臍帶放入無菌的 PBS 溶液中儲存。（注：4℃下zui多貯存 24 小時，室溫下不超過 6 小時，否則廢棄）
2. 用一個鈍頭的針頭扎入臍帶靜脈管中，用無菌的 PBS 溶液沖洗 3-5 次，將污血沖洗干凈為止。
3. 用手術鉗夾緊臍帶下端，加入 15ml 的膠原酶（1mg/ml）室溫下消化 15-20分鐘，并不時上下搖動臍帶。
4. 消化完后，將下端手術鉗松開，消化液流入一個 50 ml 無菌離心管中，用無菌的 PBS 溶液沖洗臍帶 2-3 次。
5. 將收集液離心（2000 轉/ 分）3 分鐘。
6. 倒去上清。加培養基。
歡迎您致電 華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司 ：1500 1904 520。紅榮微再-客服: 2395557778 經銷商專員
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