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Reversed-Phase and Hydrophilic Interaction Phases Combined on a Single Column

The Acclaim^® Mixed-Mode HILIC-1 column utilizes a unique silica-based, mixed-mode stationary phase that combines both reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) properties. The column functional group features a hydrophobic alkyl chain and hydrophilic diol functionality. This combination allows both hydrophobic interaction and hydrophilic interaction to be utilized to optimize separations.

• Operates in both RP and HILIC modes
• Retains highly polar molecules
• Unique selectivity complementary to RP columns
• Superior performance compared to conventional diol-based columns
• High efficiency column for high resolution separations

The Acclaim Mixed-Mode HILIC-1 columns consist of 5 μm high-purity, porous, spherical silica with 120 ?-diameter pores bonded with proprietry functional groups, which provide unsurpassed resolution and peak symmetry for a variety of polar and nonpolar molecules.

Wide Range of Applications

The Acclaim Mixed-Mode HILIC-1 column separates both polar and nonpolar molecules with selectivity complementary to RP columns and is suitable for a broad range of applications, including drug metabolites, lipids, polyethylene glycols (PEGs), ethoxylated surfactants and more.