CryoStor®, a series of cell-specific, optimized freeze media, is designed to prepare and preserve cells in ultra low temperature environments (-70ºC to -196ºC); CryoStor®, pre-formulated with DMSO, provides a safe, protective environment for cells and tissues during the freezing, storage, and thawing process. Through modulating the molecular-biological response to the cryopreservation process, CryoStor® provides for enhanced cell viability and functionality while eliminating the need for serum, proteins or high levels of cytotoxic agents.

各種規格的BioLIfe Solutions CryoStor 細胞DMSO凍存液

BioLife Solutions CryoStor®是BioLife公司特別優化的細胞凍存液，在極低溫度 (-70ºC to -196ºC)下準備和保存細胞。預混DMSO，為細胞和組織的冷凍、儲存和解凍過程提供安全的保護環境。CryoStor®通過調控凍存過程的分子生物學反應，無血清、蛋白或具有高細胞毒性的試劑，就能增強細胞活力和功能。

BioLife Solutions CryoStor CS10細胞凍存液特點

用于細胞和組織的高級生物保存液：

預混DMSO

無血清、無蛋白

USP級高質量成分、cGMP級生產、FDA master文件

滅菌、內毒素和細胞功能檢測

品牌	貨號	產品描述	規格
BioLife Solutions	210102	CryoStor CS10 Freeze Media 細胞DMSO凍存液	100mL /瓶
BioLife Solutions	210210	CryoStor CS10 Freeze Media 細胞DMSO凍存液	1000mL/袋
BioLife Solutions	210202	CryoStor CS10 Freeze Media 細胞DMSO凍存液	100mL/袋
BioLife Solutions	210374	CryoStor CS10 Freeze Media 細胞DMSO凍存液	16mL /瓶
BioLife Solutions	210373	CryoStor CS10 Freeze Media 細胞DMSO凍存液	10mL /瓶

BioLife Solutions CryoStor CS10 細胞DMSO凍存液cGMP級生產

BioLife Solutions CryoStor CS10 Freeze Media 使用與凍存方法

CryoStor® Usage and Cryopreservation Protocol

細胞準備

1) Place cells to be cryopreserved into suspension (mechanical or enzymatic dissociation)

2) Centrifuge cells to obtain cell pellet

3) Remove supernatant - Note: Remove as much culture media as possible, to reduce dilution of CryoStor® solution.

加CryoStor凍存液

4) ISOLATION: Add cold (2-8°C) CryoStor®

a. Cell concentrations: 0.5-10 x 10⁶

cells/ml for routine cell culture protocols (higher [cell] possible).

b. DMSO is pre-mixed in CryoStor® - no additives are necessary.

5) PRE-FREEZE: Incubate cell suspension at 2-8°C for approximay 10 minutes

6) NUCLEATION: Freeze samples at -70°C (many protocols utlilize -70°C and -80°C interchangeably)

a. Use a controlled rate freeze (-1°C/min) or similar protocol for most mammalian cell systems.

b. The freezing device or isopropanol container should be pre-cooled to 2-8°C.

c. Ice nucleation within the sample (seeding) should be initiated at approximay -5°C using either a liquid nitrogen burst program setting on a controlled rate freezer or mechanical agitation (flick or tap) of the cryovial/sample container after approximay15-20 min. at -70°C.

d. Freeze time (-70°C) using isopropanol containers is recommended to be 3-4 hours.

BioLife Solutions 儲存

7) STORAGE: Place samples into storage

a. Store samples at liquid nitrogen temperatures (below -130°C).

b. Sample storage at -80°C is only recommended for short-term storage (weeks to months).

細胞復蘇解凍

8) THAWING: Thaw samples quickly in a 37°C water bath

a. Sample thawing should be conducted with gentle swirling of sample until all visible ice has melted. Approximate thaw time for a 1 ml sample in a cryovial is approximay 3 minutes.

b. DO NOT allow sample to warm above chilled temperatures (0-10°C). Cryovials should be cool to the touch when removed from bath.

Passive thaw is not recommended.

9) Dilute cell/CryoStor® mixture immediay with culture media

a. Dilution procedure can be preformed in a single step.

b. The dilution media should be between 20°C and 37°C.

c. A dilution ratio of 1:10 (sample to media) or greater is recommended.

10) Plate cells in appropriate configuration

11) Place cells into culture conditions or utilize immediay

12) Viability assessment 24-hours post-thaw*

BioLife Solutions Note: To obtain an accurate measure of cell viability following cryopreservation, assessment should be performed 24 hours post-thaw and compared to non-frozen controls.

*Sample assessment immediay post-thaw with membrane integrity indicators, such as Trypan Blue, for comparative analysis of sample cell yield and viability often results in significant overestimates of cell survival.

Live/Dead fluorescent assays or metabolic assays (MTT or alamarBlue®) are recommended for more accurate viability assessment.

Visual inspection of adherent cells and cells “floating” in the media is also recommended.

BioLife Solutions CryoStor CS10細胞DMSO凍存液210102 代理銷售歡迎您致電

重慶市華雅干細胞技術有限公司，：

重慶市華雅干細胞技術有限公司以“專注干細胞研究，提升干細胞品質”為發展理念，專業從事干細胞基礎研究、干細胞再生醫學臨床應用研究及干細胞技術服務。

以上信息由企業自行提供，信息內容的真實性、準確性和合法性由相關企業負責，化工儀器網對此不承擔任何保證責任。
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干細胞無血清培養基

干細胞因子及添加物

細胞治療產品

細胞培養耗材

相關檢測試劑

其他生物試劑

細胞凍存

細胞重編程

蛋白質/多肽

抗體/抗原

小型儀器設備

其他

核酸分析

細胞株

[供應]BioLife Solutions CryoStor CS10 210102細胞DMSO凍存液