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NUCLEOSIL® NH2 / NH2 RP

Amino modified silica phases for HPLC · USP L8

Features

Polar aminopropyl modified silica for reversed phase and normal phase chromatography:
Normal phase chromatography with hexane, dichloromethane or 2-propanol as phase for polar compounds such as substituted anilines, esters, chlorinated pesticides
Reversed phase chromatography of polar compounds like carbohydrates in aqueous-organic eluent systems
Anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile)

Available pore sizes 100, 120 and 300 Å, available particle sizes 3 µm, 5 µm, 7 µm and 10 µm,
pH stability at 20 °C: 2–8, carbon content 3.5 %

Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions

Corresponding NUCLEODUR® phase see NUCLEODUR® NH2 / NH2-RP

For applications click here.

雙糖檢測

色譜柱：Nucleosil 100-5 NH2, 250×4 mm

流動相：乙腈：水=75:25

流 速：1毫升/分鐘

樣 品：1. 甲基葡萄糖苷 2. 果糖 3. 葡萄糖 4. 蔗糖 5. 乳糖 6. 麥芽糖

HPLC檢測 Curcumin （姜黃素）及相關組份
 

色譜柱：Nucleosil 100-5 NH2, 250×4.6 mm ID

流動相：96% 乙醇

流 速：1.2毫升/分鐘

檢測器：fluorescence, excitation 420nm, emission 470nm. The curcumino ids can also be detected by UV-VIS spectroscopy at 254 or 420nm.

樣 品：1. Bisde methoxycurcumin  2. Demethoxycurcumin  3.Curcumin

發酵液中多糖的檢測

色譜柱：Nucleosil 100-5 NH2, 250×4 mm

流動相：乙腈：水=65:35

流 速：2毫升/分鐘

溫 度：80℃
樣 品：1. 葡萄糖 2. 纖維素二糖 3-8.不同聚合度葡聚糖

EC standard columns for analytical HPLC

Ordering information

Eluent in column (except for NUCLEOSIL® 100-5 NH2 RP) is n-heptane. When using an eluent which is not miscible with n-heptane (e.g., water), it is necessary to rinse the column with THF first.

ID

Particle size

Pore size

Length 250 mm

EC guard
columns*

CC guard
columns**

NUCLEOSIL® 100-3 NH2 (eluent in column n-heptane)

4.6 mm

3 µm

100 Å

720275.46

721933.30

721122.40

NUCLEOSIL® 100-5 NH2 RP (eluent in column acetonitrile – water)

4.6 mm

5 µm

100 Å

720095.46RP

721155.30

721605.40RP

NUCLEOSIL® 100-5 NH2 (eluent in column n-heptane)

4.6 mm

5 µm

100 Å

720095.46

721020.30

721605.40

NUCLEOSIL® 100-10 NH2 (eluent in column n-heptane)

4.6 mm

10 µm

100 Å

720025.46

Columns/pack ->

1

3

3

* EC 4/3 guard column cartridges are used for EC columns of 4.6 mm ID with the Column Protection System guard column holder (REF 718966).
** CC 8/4 guard column cartridges are used for EC columns of 4.6 mm ID with guard column adapter EC (REF 721359).

Custom-packed EC columns with other dimensions are available on request.

NUCLEOSIL® NH2 bulk packingsOrdering information

Phase

Carbon content

Pore size

Particle size

Pack of 10 g

Pack of 100 g

NUCLEOSIL 100-5 NH2

3.5 %

100 Å

5 µm

712200.10

712200.100

NUCLEOSIL 100-10 NH2

3.5 %

100 Å

10 µm

712210.10

712210.100

NUCLEOSIL 120-7 NH2

~ 2 %

120 Å

7 µm

712610.10

712610.100

NUCLEOSIL 300-7 NH2

~ 2 %

300 Å

7 µm

712919.10

712919.100