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國際期刊 | 翌圣qPCR榮登高分雜志！circVAMP3或成肝細(xì)胞癌治療靶點(diǎn)
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Yu, Q., Liu, S., Yu, L. et al. RNA demethylation increases the yield and biomass of rice and potato plants in field trials. Nat Biotechnol (2021). （IF 54.908）
Dong W, Zhu Y, Chang H, et al. An SHR–SCR module specifies legume cortical cell fate to enable nodulation[J]. Nature, 2021, 589(7843): 586-590. （IF43）
03
Bi X, Wang K, Yang L.et al. Tracing the genetic footprints of vertebrate landing in non-teleost ray-finned fishes. Cell. (2021). (IF 38.637)
Liu S , Hua Y , Wang J , et al. RNA polymerase III is required for the repair of DNA double-strand breaks by homologous recombination[J]. Cell. (2021). (IF 38.637)
Liu C , Li X. et.al. Structure and Degradation of Circular RNAs Regulate PKR Activation in Innate Immunity. Cell. (2019). (IF 36.216)
Lu, XY.,Shi, XJ., Hu, A.et al.Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis.Nature(2020). （IF43）
Xie, F., Su, P., Pan, T., et al. Engineering Extracellular Vesicles Enriched with Palmitoylated ACE2 as Therapy [J]. Advanced Materials, 2021, IF30.849. ://doi.org/10.1002/adma.202103471
03
Liang, Y., Lu, Q., Li, W., et al. Reactivation of tumour suppressor in breast cancer by enhancer switching through NamiRNA network [J].Nucleic Acids Research 2021, IF16.9. ://doi.org/10.1093/nar/gkab626
Zhou, J., Chen, P., Wang, H., et al. Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition [J]. Molecular Therapy, 2022, IF11.454. ://doi.org/10.1016/j.ymthe.2021.10.010
Yeasen qPCR實(shí)驗(yàn)解決方案
| 凍融次數(shù) | 0次 | 10次 | 30次 | 50次 |
| Ct均值 | 23.11 | 23.17 | 23.18 | 23.25 |
| ?Ct值 | - | 0.06 | 0.07 | 0.14 |
| 方法 | 分類 | 產(chǎn)品名稱 | 貨號 |
| qPCR染料法 | 高靈敏通用性qPCR預(yù)混液（染料法） | Hieff UNICON® Universal Blue qPCR SYBR® Master Mix（男神版） | 11184ES |
| 高特異型qPCR預(yù)混液（染料法） | Hieff UNICON® Power qPCR SYBR® Green Master Mix (No Rox) | 11195ES | |
| Hieff UNICON® Power qPCR SYBR® Green Master Mix(Low Rox) | 11196ES | ||
| Hieff UNICON® Power qPCR SYBR® Green Master Mix(High Rox) | 11197ES | ||
| 高靈敏型qPCR預(yù)混液（染料法） | Hieff UNICON® qPCR SYBR® Green Master Mix (No Rox) | 11198ES | |
| Hieff UNICON® qPCR SYBR® Green Master Mix (Low Rox) | 11199ES | ||
| Hieff UNICON® qPCR SYBR® Green Master Mix (High Rox) | 11200ES | ||
| 普通型qPCR預(yù)混液（染料法） | Hieff® qPCR SYBR® Green Master Mix (No Rox ) | 11201ES | |
| Hieff® qPCR SYBR® Green Master Mix (Low Rox ) | 11202ES | ||
| Hieff® qPCR SYBR® Green Master Mix (High Rox ) | 11203ES | ||
| miRNA qPCR加A法 | Hieff® miRNA Universal qPCR SYBR Master Mix（加A法） | 11171ES | |
| miRNA qPCR莖環(huán)法 | Hieff® miRNA Universal qPCR SYBR Master Mix（莖環(huán)法） | 11170ES | |
| RNA一步法RT-qPCR（染料法） | Hifair® III One Step RT-qPCR SYBR Green Kit | 11143ES | |
| qPCR探針法 | 單重、普通型qPCR預(yù)混液（探針法） | Hieff Unicon® qPCR TaqMan Probe Master Mix | 11205ES |
| 多重、高靈敏型qPCR預(yù)混液（探針法） | Universal TaqMan multiplex qPCR master mix | 11211ES | |
| RNA一步法RT-qPCR（探針法） | Hifair® III One Step RT-qPCR Probe Kit | 11145ES |
| 名稱 | 貨號 | 規(guī)格 | 價格（元） |
| Hieff TransTM Liposomal Transfection Reagent 脂質(zhì)體核酸轉(zhuǎn)染試劑 | 40802ES01 | 100 μL | 168 |
| 40802ES02 | 0.5 mL | 738 | |
| 40802ES03 | 1.0 mL | 1308 | |
| 40802ES08 | 5×1mL | 4878 | |
| Calcium Phosphate Cell Transfection Kit磷酸鈣法細(xì)胞轉(zhuǎn)染試劑 | 40803ES70 | 200 T | 625 |
| Polybrene (hexadimethrine bromide) 聚凝胺（10 mg/ml） | 40804ES76 | 500 μL | 180 |
| 40804ES86 | 5×500 μL | 500 | |
| Hieff TransTM Suspension Cell-Free Liposomal Transfection Reagent懸浮細(xì)胞專用脂質(zhì)體核酸轉(zhuǎn)染試劑 | 40805ES01 | 100 μL | 228 |
| 40805ES02 | 0.5 mL | 948 | |
| 40805ES03 | 1.0 mL | 1678 | |
| 40805ES08 | 5×1 mL | 5268 | |
| Hieff TransTM in vitro siRNA/miRNA Transfection Reagent siRNA/miRNA體外轉(zhuǎn)染試劑 | 40806ES01 | 0.1 mL | 372 |
| 40806ES02 | 0.5 mL | 1472 | |
| 40806ES03 | 1.0 mL | 2572 | |
| Polyethylenimine Linear(PEI) MW25000 線性PEI轉(zhuǎn)染試劑MW25000 | 40815ES03 | 1 g | 1855 |
| 40815ES08 | 5×1 g | 7255 | |
| Polyethylenimine Linear(PEI) MW40000（rapid lysis）線性PEI轉(zhuǎn)染試劑（速溶型）MW40000 | 40816ES02 | 100 mg | 655 |
| 40816ES03 | 1 g | 1855 | |
| 40816ES08 | 5×1 g | 7255 |
