ORFLO Technologies總部位于美國愛達荷州，擁有多項技術，如微流控、生物信號向電子信號轉化、數據采集和分析、配套分析軟件設計等，致力于現代細胞分析技術創新和開發,在細胞分析技術競爭中處于*的地位。
ORFLOTechnologies 2010年開發*個掌上細胞計數儀，產品一經推出，很快受到業內廣泛認可。目前推出代迷你細胞計數儀Moxi Z和掌上流式細胞儀MOX

簡單介紹

世聯博研（北京）科技有限公司正式做為Orflo中國代理商，主要產品包括Moxi Flow微型流式細胞儀及MoxiZ細胞計數產品系列，迷你細胞計數儀Moxi Z和掌上流式細胞儀MOXI FLOW

產品描述

Moxi GO & Moxi GO II流式細胞儀—*

 

Product Code: MXG001_MXG002_MXG102
Moxi GO 532nm laser (MXG001)
1 PMT - 561nm/LP filter (e.g. PE, PI) - US Version
Moxi GO 488nm laser (MXG002)
1 PMT - User-swappable 525/45nm (e.g. FITC, GFP) or 561nm/LP (PE,PI) filters
Moxi GO II 488nm laser (MXG102)

2 PMT system 525/45nm (e.g. FITC, GFP) and 561nm/LP (PE, PI)

Overview

 

3 or 4 Parameter Next Gen Flow

 

Increase the pace of scientific discovery with ORFLO’s next generation Moxi Go™ or Moxi Go II™ Next Gen Flow Cytometers. With a one (Moxi GO) or two (Moxi GO II) fluorescent channels, swappable filter sets (MXG002 - Moxi GO 488nm), configurable laser the Moxi Go can be designed to meet your labs cell analysis needs.  Every assay also delivers simultaneous cell count & volume determination, using single-use flow cell.  This eliminates the hassle of traditional flow associated with cleaning, maintenance, clearing of clogs, cross contamination and occasionally replacement of bottles and tubes.  

 

Furthermore the Moxi Go™ or Moxi Go II™ use very little sample volume, 75ul's allowing you to conserver precious, expensive sample, such as stem cells.  Cell concentrations as low as 10,000 cells per ml are possible, which most experiments would enable a little as 5ul's of sample diluted in 70ul's of PBS.

 

The Moxi Go™ or Moxi Go II™ can be utilized as an assay development instrument and many powerful cell based assays can easily be optimized and run on the system.  These include:

• Cell surface immuno-labeling
• In-Cell Protein Quant
• CRISPR/Transfection Optimization Studies
• Up to 4 plex bead based ELISA's using our new on board MPX Relative Quantitation Ap
• In cell Wester Blots
• Reactive Oxidation Species Experiments
• Calein AM studies
• Phagocytosis Analysis
• Mito Potential Experiements

 

The Moxi Go™ or Moxi Go II™ come standard with an ultra-intuitive, plug-and-play interface with free OS updates as long as you own the instrument. No prior flow cytometry experience is required you simply just plug and play.

參數規格：

id	MXG001_MXG002_MXG102
Included Accessories	USB power cord, US style USB power adapter, and Type MF-S cassette pack
AC Power Type	110 VAC
Applications	Mulitplexed Bead ELISA's|In Cell Westerrns|In Cell Protein Quant|RFP| Gold Standard Cell Count and Viability|Mito Potential|ROS|Phagocytosis
Average Cell Diameter Range	3 - 16 microns Type MF-S
Battery Type	Rechargeable 3.7 V, 4500 mAh lithium ion
Cassette Types	Type MF-S
Cell Particle Concentration Range	10,000 - 1,750,000 cells/mL Type MF-S
Cell Types Tested	HEK-293|HeLa|PC12|CD3+T|CHO-K1|Cos-7|HepG2|Hybridoma|Jurkat E6-1|K562|MCF7|Mesenchymal SC|Monocyte|Mouse ESC| NIH 3T3| PBMC (cultured)|Red Blood Cells (RBC)|L5178y| C. albicans (Yeast)| S. cerevisiae Vin 13 (Yeast)|S. cerevisiae X5 (Yeast)|Wine Yeast (natural fermentaion)|S.cerevisiae (Baker's Yeast|Safale US-05 Yeast|
Data Output Formats	FCS 3.1, screen shots (.bmp), CSV
Data Storage Capacity	16Mb and 4GB microSD
Display Resolution	800 x 480 color touchscreen
Excitation Wavelengths	532 nm (MXG001) or 488nm (MXG002, MXG102)
In British Units	7 lbs
Intended Use Statement	For Research Use Only. Product is not for use in diagnostic procedures
Laser Color	Green(MXG001), Blue (MXG002, MXG102)
Measurable Dynamic Range	2 - 25 microns Type MF-S
Measurement Time	10 seconds Type MF-S
MPICell Health Ratio Moxi Viability Index MVI	Yes (Size histogram only)
Number of Detection Channels Flow parameters	MXG001 and MXG002: 3 (1 color, 1 size, 1 transmittance), MX104: 4 (2 color, 1 size, 1 transmittance)
Number of PMTs	1
Optical Detection Range	MXG001: 561/LP nm (e.g. PE, RFP), MXG002: User-swappable 525/45nm (e.g. FITC, GFP) or 561nm/LP (e.g. PE, RFP), MXG102: 525/45nm (e.g. FITC, GFP) and 561nm/LP (e.g. PE, RFP)
Particle Size Detection Method	Impedimetric (Coulter Principle)
Platform	Open platform: 561nm/LP (PI, PE, DS Red, Sytox Orange, 7 AAD, Nile Red, Rhodamine Red, Sun Coast Yellow, PE/Cy5), 525/45nm (FITC, GFP, Alexa Fluor 488nm, Calcein)
Pre-Programmed Tests	Mulitplexed Bead ELISA's|In Cell Westerrns|In Cell Protein Quant|RFP|Gold Standard Cell Count and Viability|Mito Potential|ROS|Phagocytosis
Resolution Histogram Bins	1000
Sample Type	Beads|Cell Preparations
Sample Volume	75 µL
Supported Connectivity	USB on-the-go
Useable Cell Volume	14 - 2145 fL Type MF-S
Weight	3.18 kg

How It Works

The operating principle behind the Moxi Go & Moxi GO II Flow Cytometers is a unique combination of Coulter-style cell size determination with simultaneous fluorescence detection. As cells flow single file through the microfabricated single-use flow cell the volume of each particle is measured at the exact same time as their primary fluorescence is measured using either a 532nm (MXG001) or 488nm (MXG002) solid state diode laser with and with the following emission filters - MXG001: 561/LP nm (e.g. PE, RFP), MXG002: User-swappable 525/45nm (e.g. FITC, GFP) or 561nm/LP (e.g. PE, RFP), MXG102: 525/45nm (e.g. FITC, GFP) and 561nm/LP (e.g. PE, RFP). Thousands of cells are measured in the 10 second read time and the data are plotted in a gradient density scatter plot as Cell size (volume) vs. Fluorescence (PMT voltage). Gating is easily performed on the unit using a interactive touch display, and the resulting live/dead ratios are automatically calculated (depending on the app selected). The analyzed data can also be displayed as a two color size histogram. Total volumetric cell counts are automatically determined for each test by precisely measuring the volume of fluid being analyzed.

Step 1:

Select desired app, insert the cassette and close the doors.

Step 2:

Once auto-alignment is complete, open the top door and pipette 75μl of labelled sample into the cassette.

Step 3:

Close the top door, assays run automatically and results are generated in ~10 seconds. Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into Moxi GO, and load Sample 2.

Data:

Data can be displayed on the unit in both a color density scatter plot and a two color size histogram. Simply drag gates using the intuitive touch display for instant live/dead ratio calculations and each of the gated volumetric cell counts (i.e., total population, live population, and dead population (Viability App). The mean cell volume for the gated populations is also automatically displayed on the unit. Results from each test are stored in the standard FCS 3:1 format and can be viewed using any flow cytometry analysis package. The actual Moxi Flow screenshots from each assay (dot plots and histograms) are also stored in bitmap format for use online. Hundreds of files can be stored on each Moxi GO and are easily transferred to a Mac or PC using USB on-the-go. No aditional software is required.

簡單介紹

世聯博研（北京）科技有限公司正式做為Orflo中國代理商，主要產品包括Moxi Flow微型流式細胞儀及MoxiZ細胞計數產品系列，迷你細胞計數儀Moxi Z和掌上流式細胞儀MOXI FLOW

產品描述

                       備有庫存現貨，隨時發貨

 

ORFLO Technologies總部位于美國愛達荷州，擁有多項技術，如微流控、生物信號向電子信號轉化、數據采集和分析、配套分析軟件設計等，致力于現代細胞分析技術創新和開發,在細胞分析技術競爭中處于的地位。

ORFLOTechnologies 2010年開發*個掌上細胞計數儀，產品一經推出，很快受到業內廣泛認可。目前推出代迷你細胞計數儀Moxi Z和掌上流式細胞儀MOXI FLOW。

 

世聯博研（北京）科技有限公司正式做為Orflo中國代理商，主要產品包括Moxi Flow微型流式細胞儀及MoxiZ細胞計數產品系列等。

 

配套耗材：

 

一、Moxi Z 微型自動細胞計數儀配套芯片(Cell Counting Cassettes)

芯片覆蓋直徑范圍（2-34微米）內的所有細胞樣品，無需根據細胞大小進行更換，

每個芯片可做2個測試。當樣品1完成后，只需取出，將芯片的另一端插入Moxi Z，加入樣品2。

在一次性盒式芯片中具有更高的性能和簡單性。

Moxi Z*的保護薄膜細胞計數盒可在8秒鐘內提供高精度，可重復的細胞計數和細胞大小分析（S型盒帶15秒）。受計數系統使用的金標準Coulter原理的啟發，Moxi盒每個測試通過一個細胞感測區域流動數千個細胞，以準確捕獲樣品中每個單個細胞的基于阻抗的體積測量。基于圖像的細胞計數方法，單個細胞的基于阻抗的細胞計數被證明是超過95％的準確度，而75％的準確度。

沒有系統污染和集成預過濾器防止堵塞。

Moxi盒式芯片設計有*的“細胞篩”，以zui大限度地減少細胞結塊和堵塞。另外，Moxi盒含有100％的樣品在盒體內，消除了系統污染和滅菌的可能性。

1、Moxi Z S型細胞計數芯片（Moxi Z Type S Cassettes）

 

The Type S cassette is ideal for accuray measuring most yeast and other particles as small as 3 µm and up to 20 µm in average diameter including some algae and protozoa. Since the technology is based on a volumetric measurement, non-spherical particles (14 - 4,200 fL) can also be measured accuray.

S型細胞計數芯片非常適用于測量大多數酵母和其他顆粒，平均粒徑小至3μm，直徑達20μm，包括一些藻類和原生動物。 由于該技術基于體積測量，因此也可以準確測量非球形顆粒（14-4,200fL）。

 

 

Cell Counting Cassettes

 

Higher Performance & Simplicity in One Disposable Cassette.
Moxi Z's unique, patent-protected thin-film cell count cassettes provide highly accurate, repeatable cell counts and cell size analysis in under 8 seconds (15 seconds for Type S cassette). Inspired by the gold-standard Coulter Principle used in high-end counting systems worldwide, the Moxi cassette flows thousands of cells per test through a cell sensing zone to accuray capture the impedance-based volumetric measurement of each individual cell in the sample. Impedance-based cell counting of individual cells is proven to be over 95% accurate versus a 75% accuracy with image-based cell counting methods. 

No system contamination and integrated pre-filter for clogging prevention. 
Moxi cassettes are designed with a unique "cell sieve" to minimize cell clumping and clogging. In addition, Moxi cassettes contain * of your sample within the cassette body, eliminating the possibility for system contamination and sterilization. Cassettes are simply and easily discarded when testing is finished.

 

Moxi Z Type S Cassettes

The Type S cassette is ideal for accuray measuring most yeast and other particles as small as 3 µm and up to 20 µm in average diameter including some algae and protozoa. Since the technology is based on a volumetric measurement, non-spherical particles (14 - 4,200 fL) can also be measured accuray.

 

Moxi Z Type S Pack (25 Cassettes)

  |  

Moxi Z Type S Box (250 Cassettes)

 

 

Moxi Z Type M Cassettes

The Type M cassette is ideal for accuray measuring mammalian cells or other particles 4-25 microns in average diameter. Since the technology is based on a volumetric measurement, non-spherical particles (34-8,180 fL) can also be measured accuray.

 

Moxi Z Type M Pack (25 Cassettes)

  |  

Moxi Z Type M Box (250 Cassettes)

 

 

 

 

 

 

MOXI Z System Check Beads (5 mL) Product Code: MXA005 MOXI Z System Check Beads (5 mL)   Sh List
Related Products Accessories Cassettes Instruments   Specifications   id MXA005 Application Notes Can be used to check proper system function. Bead Diameter 10 micron Compatible Instruments Moxi Z Concentration 1.1 e+5 beads/mL Formulation Orflo Diluent Intended Use Statement For Research Use Only. Product is not for use in diagnostic procedures Material of Construction Polystyrene Overall Dimensions 22.1 mm^3 Sterility Non-sterile Weight 5 mg

 

Moxi Z Diluent Product Code: MXA006 Moxi Z Diluent, 100 mL
Related Products Reagents Accessories Cassettes Instruments   Specifications   id MXA006 Application Notes Moxi Z Diluent is special formulated for use with the Moxi Z system for optimal sizing of particles and for operation with the instrument's Small Particle Mode (SPM). Applications Cell Dilution Applications Abbreviation Cdil Cassette Types Type M|Type S Compatible Instruments Moxi Z Formulation Proprietary Intended Use Statement For Research Use Only. Product is not for use in diagnostic procedures Kit Components One 100 ml bottle of diluent Number of Tests n/a Overall Dimensions 52 L x 52 W x 106 H (mm) Protocol Pellet and resuspend cells in Moxi Z diluent or dilute sample with >90% Moxi Z diluent. 2. Pipette 10 uL of sample into tube 3. Pipette 90 uL of Orflo Diluent into tube 4. Gently mix resulting solution to ensure cells are evenly mixed 5. Pipette 75 uL of resulting solution into Moxi Z Cassette, or 50 uL into Moxi Flow cassette 6. Touch screen to run test Sample Type Mammalian Cells|Large Yeast|Large Algae|Protozoa Sterility Non-Sterile Storage Stability AND Handling Store at 2-8C. Use sterile technique when handling. Dispose of waste product in compliance with federal, state and local regulations. Do not inhale or ingest. Avoid contact with eyes and skin, flush affected area with water for at least 15 minutes Vendor ORFLO Weight 152 g

 

 

Moxi Z Diluent, sample Product Code: MXA009 Moxi Z Diluent,  5 mL See Product Specifications
Related Products Reagents Accessories Cassettes Instruments   Specifications   id MXA009 Application Notes Moxi Z Diluent is special formulated for use with the Moxi Z system for optimal sizing of particles and for operation with the instrument's Small Particle Mode (SPM). Applications Cell Dilution Applications Abbreviation Cdil Cassette Types Type M|Type S Compatible Instruments Moxi Z Formulation Proprietary Intended Use Statement For Research Use Only. Product is not for use in diagnostic procedures Kit Components One 5 ml bottle of diluent Number of Tests 75 Overall Dimensions 25 W x 45 H (mm) Protocol Pellet and resuspend cells in Moxi Z diluent or dilute sample with >90% Moxi Z diluent. 2. Pipette 10 uL of sample into tube 3. Pipette 90 uL of Orflo Diluent into tube 4. Gently mix resulting solution to ensure cells are evenly mixed 5. Pipette 75 uL of resulting solution into Moxi Z Cassette, or 50 uL into Moxi Flow cassette 6. Touch screen to run test Sample Type Mammalian Cells|Large Yeast|Large Algae|Protozoa Sterility Non-Sterile Storage Stability AND Handling Store at 2-8C. Use sterile technique when handling. Dispose of waste product in compliance with federal, state and local regulations. Do not inhale or ingest. Avoid contact with eyes and skin, flush affected area with water for at least 15 minutes Vendor ORFLO Weight 11 g

Orflo Accutaseª Product Code: MXA020 Orflo Accutase cell detachment solution, 1X, 100 mL
Supporting Resources Related Products Reagents Assays Antibodies Cassettes Instruments   Specifications   id MXA020 Application Notes Recommended for the detachment of adherent cells and dissociation of mammalilian cell lines and primary cells Applications Cell Detachment Applications Abbreviation Cdet Cassette Types Type M|Type S|Type MF-M|Type MF-S Cell Types Tested fibroblasts|keratinocytes|vascular endothelial cells|hepatocytes|vascular smooth muscle cells|hepatocyte progenitors|primary chick embryo neuronal cells|bone marrow stem cells|adherent CHO and HNK cells|macrophages|293 cells|L929 cells|immortalized mouse testicular germ cells|MIC5|3T3|Vero|COS|HeLa|NT2|MG63|M24 and A375 metastatic melanoma|gliomas U251|D54|HT1080 fibrosarcoma cells|HT29 colon cancer cells|Sf9 insect cells|human embryonic stem cells|human mesenchymal stem cells|and human neural stem cells. Compatible Instruments MoxI Z|Moxi Flow|zEPI Flow Formulation Accutase® enzymes in Dulbecco's PBS containing 0.5 mM EDTA and phenol red. 1X concentrate, no preservatives. Intended Use Statement For Research Use Only. Product is not for use in diagnostic procedures Kit Components One 100ml bottle of cell dissassociation reagent Number of Tests n/a Overall Dimensions 52 L x 52 W x 106 H (mm) Protocol 1. Place 100ml Accutase bottle in room temp water bath or thaw overnight in fridge 2. For Adherent Cells: a. Remove cell media b. Add 1.5ml Accutase per 25cm2 of flask area c. incubate at RT or 37C until cells detach (typically 5-15min) d. Pipette triturate to break apart clusters 3. For suspension cell aggregates: a. Pellet cells at 300xg, 5min b. Resuspend pellet in 1-2ml Accutase c. Incubate 15min d. Pipette triturate to break apart clusters 4. Count cells on the Moxi Z, Moxi Flow, or Zepi Flow (be sure to dilute samples into standard operating range of specific cassette, if they are above the stated concentration limits) Sample Type Primary Mammalian cells|immortalized adherent cells|non-ES cell types Sterility Sterile Storage Stability AND Handling Stable at -20C. After thawing can be stored for up to 2 months at 2-8C. Tradename ACCUTASE™ is a registered trademark of Innovative Cell Technologies Vendor ORFLO Weight 159 g

 

 

Orflo Accumaxª Product Code: MXA021 Orflo Accumax, cell dissassociation reagent, 10X, 100 mL, sterile
Supporting Resources Related Products Reagents Assays Antibodies Cassettes Instruments   Specifications   id MXA021 Application Notes Recommended for use with the Moxi Z and Moxi Flow platforms when working with clumpy aggregated cells. It is ideal for generating single cell suspensions from clumped cell cultures, detachment of cells from primary tissue and detachment of cells from plastic tissue culturware. Accumax is specifically designed for our combination fluorescence amp impedance based flow cytometers. Applications Cell Detachment Applications Abbreviation Cdet Cassette Types Type M|Type S|Type MF-M|Type MF-S Compatible Instruments MoxI Z|Moxi Flow|zEPI Flow Formulation 10X proprietary solution of proteolytic, collagenolytic and DNase enzymes and does not contain mammalian or bacterial derived products. Intended Use Statement For Research Use Only. Product is not for use in diagnostic procedures Kit Components One 100ml bottle of cell dissassociation reagent Number of Tests n/a Overall Dimensions 52 L x 52 W x 106 H (mm) Protocol 1. Place 100ml Accumax bottle in room temp water bath or thaw overnight in fridge 2. For Adherent Cells: a. Remove cell media b. Add 1.5ml Accumax per 25cm2 of flask area c. incubate at RT or 37C until cells detach (typically 5-15min) d. Pipette triturate to break apart clusters 3. For suspension cell aggregates: a. Pellet cells at 300xg, 5min b. Resuspend pellet in 1-2ml Accumax c. Incubate 15min d. Pipette triturate to break apart clusters 4. Count cells on the Moxi Z, Moxi Flow, or Zepi Flow (be sure to dilute samples into standard operating range of specific cassette, if they are above the stated concentration limits) Sample Type Primary Mammalian cells|immortalized adherent cells|Human ES cells|Rodent ES Sterility Sterile Storage Stability AND Handling Stable at -20C. After thawing can be stored for up to 2 months at 2-8C. Tradename ACCUMAX™ is a registered trademark of Innovative Cell Technologies Vendor ORFLO Weight 159 g