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世界*實驗材料供應(yīng)商BPS Bioscience正式授權(quán)上海起發(fā)為其中國代理，BPS Bioscience 在一直是行業(yè)的*,一直為廣大科研客戶提供zui為的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為中國科研客戶帶來的產(chǎn)品，的服務(wù)， 簽約BPS Bioscience 就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們zui大的收獲
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美國BPS Bioscience公司是一家快速發(fā)展的企業(yè)，總部在California.產(chǎn)品遠銷世界各地.BPS提供產(chǎn)品和服務(wù),主要方向是蛋白激酶,磷酸激酶，HDAC等.
BPSzui大的藥物發(fā)現(xiàn)用重組酶的供應(yīng)商，并提供蛋白表達和基于細胞和生物化學(xué)檢測的產(chǎn)品定制服務(wù)和化合物篩選服務(wù)。BPS在不斷擴大其藥物發(fā)現(xiàn)產(chǎn)品。
BPS還提供了自定義的蛋白表達，生物化學(xué)和基于細胞的檢測，化合物篩選服務(wù)。 BPS是顧客導(dǎo)向。以及為客戶提供服務(wù)，我們傾聽您的需要定制我們的服務(wù)，以滿足您的具體情況。
通過BPS的重點在技術(shù)發(fā)展的zui前沿，的質(zhì)量生命科學(xué)產(chǎn)品和服務(wù)提供及時，這將有助于我們的客戶加快藥物發(fā)現(xiàn)和開發(fā)用于治療人類疾病。
EZH2/EED/SUZ12/RbAp48/AEBP2, FLAG-tag, His-tag
Catalog #: 51004
Size: 50 µg
Species: Human
Host Species: Sf9 insect cells
MW: 325 kDa (complex)
Genbank #: NM_004456 (EZH2), NM_003797(EED), NM_015355 (SUZ12), NM_153207(AEBP2), NM_005610(RbAP48)
Tag(s): N-terminal FLAG-tag(EED), N-terminal His-tag (other components)
a.a: 2–end (all components)
Background:
EZH2 is the catalytic subunit of the PRC2/EED-EZH2 protein complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 protein complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
Description:
Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end, with N-terminal His-tag, MW= 86 kDa, human EED (NM_003797), a.a. 2-end, with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12 (NM_015355), a.a. 2-end, with N-terminal His-tag, MW = 87 kDa, Human AEBP2 (NM_153207), a.a. 2-end, with N-terminal His-tag, MW= 53 kDa, and human RbAp48 (NM_005610), a.a. 2-end, with N-terminal His-tag, MW = 48 kDa, co-expressed in a baculovirus expression system.
UniProt Q15910
同義字（詞）：EZH2 Protein Complex, EZH2, PRC2, Enhancer Of Zeste Homolog 2; RBAP48, Retinoblastoma Binding Protein 4 (RBBP4), NURF55, Chromatin Assembly Factor 1 Subunit C, CAF1 p48; Embryonic Ectoderm Development, hEED, EED, WAIT-1; SUZ12, Suppresso
Specific Activity: 0.08 pmol/min/µg
測定條件：50 µl reaction mix (20 mM phosphate buffer pH 7.4, 0.05% Tween-20, 20 µM S-adenosylmethionine, and 50-200 ng enzyme complex) add to the wells coated with the substrate. Incubate for 1 hr. Add antibody against methylated K27 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.
Format: Aqueous buffer solution
儲存/穩(wěn)定性：>6 months at -80°C.
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Morin, R., et al. (2010). et al., Nature Genetics. 42, 181 - 185.
2. Varambally, S. et al. (2008). Science 322 (5908), 1695-1699.
3. Rakotobe, D. et al. (2008). Virol. J. 5, 32.
Application Reference(s):
1. Arcand, M. et al., Poster presented as part of the BioTechniques Fall 2011 Epigenetics Meeting. , et al., ""Development of High-Throughput Assays to Study Methylases, Demethylases and Deacetylases."" Poster presented as part of the BioTechniques Fall 2011 Epigenetics Meeting.
2. Cohen, A. et al., Weaver Syndrome-Associated EZH2 Protein Variants Show Impaired Histone Methyltransferase Function In Vitro (2016)
3. Yap, D.B, et al., Somatic mutations at EZH2 Y641 act dominantly through a mechanism of selectively altered PRC2 catalytic activity, to increase H3K27 trimethlyation. (2010)Blood. 117:2451-2459.
警告（S）：避免凍融循環(huán)
科學(xué)類別：甲基
產(chǎn)品類別：重組蛋白
我們公司zui大優(yōu)勢是強大的采購，
1：基本什么都能進口，血清，抗體，耗材，還有部分限制進口的，
2：貨品全，現(xiàn)經(jīng)營過700多個品牌，基本所有生物試劑耗材都可以進口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，
3：提供加急服務(wù)，一般1-2周到貨，超過時限加急費全免
4：價格公道，絕大部分價格有優(yōu)勢，當(dāng)然不能保證100%產(chǎn)品都是,因為意味著沒有服務(wù).
5：良好的信譽，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大 交大 復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥 ，fisher等500多家公司
6：我們*代理的品牌有：Antibody Research Corporation，arcticzymes，Biorelevant，AmberGen, Inc. ，clemente-associates，clodronaiposomes，Columbia Biosciences，enzyme research，Gene Bridges GmbH，Genovis，AmberGen, Inc. Biotechnology GmbH，Haematologic Technologies HTI Haemtech，hookelabs，Immudex，Innovative Research of America，inspiralis ，List Biological Laboratories, Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-Freez Biologicals，pentapharm，progen，Protein Ark，QA-Bio,Inc，QA-Bio,IncQuickZyme Biosciences，Teknova，TriLink BioTechnologies, Inc.，Zyagen Laboratories 等
7：我們還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發(fā)，歡迎合作。
