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CAS號9001-12-1膠原酶Ⅰ
貨號：C8140
規格：100mg/支
外觀（性狀） : 棕色或棕褐色結晶性粉末
儲存條件 : -20℃
純度 : 0.25-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid
英文名稱 : CollagenaseⅠ
單位 : 支
別名 : 梭菌肽酶 A
| 商品貨號： | 商品品牌： | 規格 | 基本售價： | 選擇規格 |
|---|---|---|---|---|
| C8140-100mg | Solarbio | 100mg | 440.00元 |
說明：膠原酶Ⅰ用于上皮、肺，脂肪和腎上腺組織細胞的分離
膠原蛋白酶 來源于溶組織梭菌
Collagenase from Clostridium histolyticum
for general use, Type I, 0.25-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid
Biochem/physiol Actions
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
The collagenase product is a mixture of enzymes secreted by C. histolyticum, with different products differentiated by the relative ratios of the 10-18 components found in the secreted enzymes. The main components are two collagenases, clostripain, and a neutral protease. The synergistic action of these enzymes degrade collagen and other intracellular materialThe action of both collagenase enzymes and the neutral protease is necessary for effective release of cells from tissue. Various types of collagen are the natural substrates for collagenase.
Caution
As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL) and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains * activity over 7 hours when held on ice.
Preparation Note
Equivalent to first 40% ammonium sulfate fraction of Mandl, I., et al., J. Clin. Invest., 32, 1323 (1953).
Unit Definition
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.
Application
This product is suitable for the disaggregation of human tumor, mouse kidney, human adult and fetal brain, lung and many other epithelia tissues. It has also been shown to be effective in liver and kidney perfusion studies, digestion of pancreas, isolation of nonparenchymal rat liver cells and hepatocyte preparation. Collagenase has also been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products. This enzyme has been tested for the release of heptatocytes at a concentration of approximay 1 mg/mL. Concentrations for digestion range from 0.1 to 5 mg/mL.
Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Cell Dissociation, Cell Dissociation and Cell Lysis,
Collagenases, Core Bioreagents, Crude Collagenase For General Use, Enzyme Class Index, Enzymes, Proteases, Proteases & Protein Sequencing, Proteolytic Enzymes, Proteolytic Enzymes and Substrates, Research Essentials, 生化試劑, 酶、抑制劑和底物
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form essentially salt-free, lyophilized powder
solubility TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble1-2 mg/mL at 37 °C
storage temp. −20°C
CAS號9001-12-1膠原酶Ⅰ訂購說明：
1、絕大部分產品備有現貨，一般情況下都能訂貨當日發貨。
2、部分非常用產品，需提前1－2日預訂，海外期貨則需要提前3-6周預訂。
3、部分產品價格會因貨期、批次等因素發生變化，若有變動以訂貨當日新價格為準。
4、每日訂單截止時間為16點整，部分城市可到17點整，因超過截止時間造成當日不能發貨的將于次日安排發貨。
5、請辦理完貨款后，將收據、底單等連同收貨人的地址、姓名、等以傳真、、短信、等形式通知我們。
CAS號9001-12-1膠原酶Ⅰ運輸說明：
1、極低溫產品：極低溫產品運輸過程中加裝干冰運輸。用干冰把產品包裹起來，再用泡沫盒密封，膠帶層層粘住泡沫盒，放入索萊寶的箱子，然后交付給合作快遞，安全快速高效放心的送客戶的手中，保證產品的性質穩定如一，為您的實驗保駕護航。
2、低溫產品：低溫產品運輸過程中加裝索萊寶冰袋運輸。事先用冰袋把產品包裹起來，再使用泡沫盒密封，用膠帶嚴實密封泡沫盒，再放入索萊寶的箱子（保溫效果少可以持續一周），然后交付給合作快遞，安全快速高效放心的送客戶的手中，保證產品的性質穩定如一，為您的實驗保駕護航。
3、常溫產品：常溫產品運輸過程中無需加冰或者特殊包裝。產品由公司庫房人員快速配貨，準確快速高效的快遞保證產品快速送達您的手中。
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