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18906011628
當(dāng)前位置：> 供求商機(jī)> HGF Human/ 重組人肝細(xì)胞生長因子
| Synonyms | Scatter Factor （SF）, Hepatopoietin （HPTA）, HGF, HGFB, F-TCF. |
| Introduction | Hepatocyte Growth Factor （HGF） is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis. |
| Description | Hepatocyte Growth Factor Human Recombinant produced in Baculovirus is a heterodimer, non-glycosylated, polypeptide chain consisting an ?-chain of 463 amino acids and b-chain of 234 having a total molecular mass of 78.0 KDa. The HGF is purified by proprietary chromatographic techniques. |
| Source | Insect cells. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | The sterile protein powder （1 mg/ml） is lyophilized from a solution containing 50mM Acetic Acid. |
| Solubility | It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Purity | Greater than 90.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Amino acid sequence | Agrees with the sequence of native human HGF. |
| Biological Activity | The activity was assayed for scattering activity in the MDCK cell assay.The ED50 for this effect is typically at 0.5-1.0 ng/ml. |
| Protein content | Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a calibrated solution of HGF as a Reference Standard. |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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