上海金鵬分析儀器有限公司
a protein purifica-tion system
檢測樣品：Theharvestedcells
檢測項目：分離純化
方案概述：The harvested cells were resuspended in the lysis buffer con taining 50?mM Tris–HCl (pH 8.0), 100?mM NaCl, 1?mM CuCl2, 1?mg/L of lysozyme, and 10?µg/L of benzonase.
The harvested cells were resuspended in the lysis buffer containing 50 mM Tris–HCl (pH 8.0), 100 mM NaCl, 1 mM CuCl2, 1 mg/L of lysozyme, and 10 µg/L of benzonase. After 30-min incubation at ambient temperature, the cells were sonicated and the lysates were clarified by centrifugation at 8000 g for 20 min at 4 °C, and then loaded onto an immobilized metal affinity chromatography (IMAC) column (BioRad, Hercules, CA, USA) equipped with a protein purification system (JP-blupadStar, Shanghai, China).
相關產品清單
溫馨提示：
1.本網展示的解決方案僅供學習、研究之用，版權歸屬此方案的提供者，未經授權，不得轉載、發行、匯編或網絡傳播等。
2.如您有上述相關需求，請務必先獲得方案提供者的授權。
3.此解決方案為企業發布，信息內容的真實性、準確性和合法性由上傳企業負責，化工儀器網對此不承擔任何保證責任。
最新解決方案
- Ultimate XB-C30測試BP193含苯環鹵素樣品的應用報告
- Ultimate LP-C18測試雜質溶液實驗報告
- Ultimate Polar RP測定乙酰磺胺酸鉀的應用報告
- Ultimate Polar RP 檢測Resolution solution的應用報告
- Ultimate LP-C18 測試AST4438的實驗報告
- Ultimate Cellu-D 測試的比克替拉韋鈉手性的實驗報告
- Ultimate LP-C18測定14種羥基酸含量
- Xtimate C18測定01020304有關物質
- Ultimate AQ-C18測定Fmoc-異亮氨酸、Fmoc-亮氨酸、Fmoc-纈氨酸有關物質
- Ultimate XB-C18測定中間體2的應用報告
