M系列調制葉綠素熒光成像系統IMAGING-PAM
具體成交價以合同協議為準
- 公司名稱 上海澤泉科技股份有限公司
- 品牌
- 型號
- 產地 進口
- 廠商性質 代理商
- 更新時間 2025/7/10 13:07:41
- 訪問次數 12264
聯系方式：沈經理15026947287 查看聯系方式
聯系我們時請說明是化工儀器網上看到的信息，謝謝!
傳統的光纖型調制熒光儀（如pam-2100、mini-pam等）只能測量葉片上一個點的光合活性。利用一個點的數據代表一個葉片，利用一個葉片代表一個植株，進而代表一個群體（如森林、大田作物等），這種方法的誤差是比較大的。
從1980年代末期開始，科研人員就期望能通過成像型熒光儀來測量全葉片光合活性，并進行了不懈的嘗試，但受技術上的限制，所設計的儀器無法商品化或商品化了但得不到大家認可。其中一個很重要的原因就是能夠發出飽和脈沖水平強光的二極管（led）尚未面世。要利用調制熒光技術測量全葉片水平的光合作用，首先要保證葉片上任何一點所接受到的光強必須是*相同的。調制熒光技術要求光源必須能發出很強的飽和脈沖光。鹵素燈能發出很強的光，但其光場非常不均勻，根本不能用于成像！裝在一個平面上的led陣列發出的光很均勻，但在2000年前，能發出*光合有效輻射（par）的led根本沒有面世！
2000年，能發出*par的藍光led面世。2001年，zui的調制熒光儀制造商德國walz公司設計制造了真正的*臺多功能調制熒光成像系統imaging-pam。imaging-pam采用*發光led作為光源，保證葉片表明受光均勻且光強足夠強；imaging-pam采用ccd作為檢測器，能檢測葉片上每個像素的光合作用；imaging-pam秉承了walz公司pam系列熒光儀的一貫優點，功能強大，測量參數多，操作極其簡單，一面世就受到植物學家的青睞，迅速占領市場。
2005年，walz又推出了m系列imaging-pam，一個主機可以連接不同的探頭（microscopy-，micro-，mini-和maxi-探頭），分別在130×150 um、3.5×4.5 mm、24×32 mm或10×13 cm的面積上測量熒光成像。現在，只需一個主機連接不同的探頭，即可滿足從單細胞到全葉片，從分子生物學到生態學研究的全面需要。
m系列imaging-pam不同版本的比較
maxi-版 | mini-版 | micro-版 | microscopy-版 |
|
|
|
|
|
|
|
|
成像面積10×13 cm | 成像面積24×32 mm | 成像面積3.5×4.5 mm | 成像面積130×150 um |
放大1.5倍 | 放大6倍 | 放大45倍 | 放大130-1300倍 |
功 能
＊ 一個主機連接不同的探頭可滿足從單細胞到全葉片、從分子生物學到生態學的不同需求
＊ 全葉片光合作用分析（熒光成像），可測熒光誘導曲線并進行淬滅分析
＊ 可測快速光響應曲線（120 s內完成，比光合放氧和氣體交換等技術快得多）
＊ 葉片光合作用的橫向異質性檢測
＊ *相同的條件下同時測量多個樣品（植物、地衣、苔蘚、微藻等）
＊ 遺傳育種、突變株篩選的強大工具
＊ 不同的測量面積，不同的分辨率
＊ 可利用多孔板（如96孔板）做多個微藻樣品的同時成像
＊ 脅迫損傷的早期檢測
＊ 不連接顯微鏡即可測量綠色熒光蛋白（gfp）熒光
＊ 可測量葉片吸光系數
測量參數
＊ 以上所有參數均可成像
＊ 吸光系數abs和新參數ql、y(npq)和y(no)的成像是imaging-pam*的
＊ 生態毒理學研究中，選一個參考點，可以直接求出其它處理（如農藥）的受抑制程度inh.
各種熒光參數的成像是將0.0（黑色）至1.0（紫色）的數值轉換成顏色來顯示的。
應用范圍
| ＊ 環境科學 ＊ 水生生物學 ＊ 海洋與湖沼學 ＊ 生態毒理學 ＊ 園藝學 ＊ 農業科學 ＊ 林學 | ＊ 環境科學 ＊ 水生生物學 ＊ 海洋與湖沼學 ＊ 生態毒理學 ＊ 園藝學 ＊ 農業科學 ＊ 林學 |
|
m系列imaging-pam不同版本介紹
maxi-版 |
|
|
|
|
|
測量盆栽植物 | 測量離體葉片 | 測量微藻樣品 | 新增鏡頭可調放大倍數 |
|
|
|
|
y(npq) | ps/50 | f | 96個微藻樣品成像 |
mini-版 |
|
由于mini-探頭的便攜式設計，使其特別適合野外應用。由于mini-探頭的成像面積僅為maxi-探頭的1/16，因而前者發出的zui大光強更大，但耗電卻小得多。mini-探頭可以安裝在光合儀gfs-3000的葉室3010-s上，同步測量全葉片氣體交換和熒光成像，并且其光源可由gfs-3000控制，達到真正的同步測量。
mini-探頭采用1/3”數碼相機ccd（640×480象素）和f1.2/f=12mm物鏡。其設計目的為測量固定距離下的熒光成像。
|
|  |
|
與光合儀gfs-3000連用 | 長時間測量可裝在三角架上 | fm | qn |
micro-版
微探頭，成像面積3.5×4.5 mm
調制熒光成像系統的micro-探頭是一個極便攜的探頭，采用整合式cosmicar-pentax cctv物鏡（f1.4/f=16mm），直接安裝在數碼ccd（1/3” chip, 640×480像素）上。
micro-探頭只配備一個luxeon led（藍光，450 nm）和一個特制雙色分光鏡，類似于落射熒光顯微鏡。
盡管成像面積只有3.5×4.5 mm，但45倍的放大率卻允許對葉片熒光成像的異質性分析達到支脈（minor veins）級。同時還可提供一個特制版本用于測量gfp的成像。 
micro-探頭還可安裝在標準版imaging-pam（2001年設計）主機上。該探頭提供x-y軸可調的樣品臺。其設計目的為測量固定距離下的熒光成像。
|
|
|
fo | gfp成像 | 微探頭直接安裝在ccd上 |
microscopy-版
顯微探頭，成像面積130×150 um
必須與特制落射熒光顯微鏡（hund或zeiss）結合使用，該顯微鏡可以提供激發光并檢測熒光
imag-max/k（數碼相機ccd）[1392×1040象素，4象素組合（binning）技術]可以提供高靈敏度。
探頭標準配置是一個*luxeon led（450-480 nm），用于提供測量光、光化光和飽和脈沖。目前已可提供rgb探頭（紅-綠-藍-白led光源），它采用了phyto-pam技術，可以顯微鏡下自動對藍藻、綠藻、硅/甲藻、紅藻進行分類并測量光合作用。
|
|
分類，紅色為硅藻，綠色為絲狀綠藻 | 光合，fv/fm活性，可區分細胞不同部位的活性 |
部分文獻
1. abrego d, ulstrup ke, willis bl, van oppen mjh: species–specific interactions between algal endosymbionts and coral hosts define their bleaching response to heat and light stress proc. r. soc. lond. b 2008;275:2273-2282.
2. abreu me, munné-bosch s: hyponastic leaf growth decreases the photoprotective demand, prevents damage to photosystem ii and delays leaf senescence in salvia broussonetii plants. physiologia plantarum 2008;134:369-379.
3. abreu me, munné-bosch s: salicylic acid may be involved in the regulation of drought-induced leaf senescence in perennials: a case study in field-grown salvia officinalis l. plants environmental and experimental botany 2008:in press.
4. ainsworth td, hoegh-guldberg o, heron sf, skirving wj, leggat w: early cellular changes are indicators of pre-bleaching thermal stress in the coral host journal of experimental marine biology and ecology 2008;364:63-71.
5. ali na, juneau p, didur o, perreault f, popovic r: effect of dichromate on photosystem ii activity in xanthophylldeficient mutants of chlamydomonas reinhardtii. photosynthesis research 2008;95:45-53.
6. calatayud á, gorbe e, roca d, martínez pf: effect of two nutrient solution temperatures on nitrate uptake, nitrate reductase activity, nh4+ concentration and chlorophyll a fluorescence in rose plants environmental and experimental botany 2008:in press.
7. ehlert b, hincha dk: chlorophyll fluorescence imaging accuray quantifies freezing damage and cold acclimation responses in arabidopsis leaves. plant methods 2008;4:1-7.
8. escher bi, bramaz n, mueller jf, quayle p, rutishauser s, vermeirssen elm: toxic equivalent concentrations (teqs) for baseline toxicity and specific modes of action as a tool to improve interpretation of ecotoxicity testing of environmental samples. journal of environmental monitoring 2008;10:612-621.
9. escher bi, bramaz n, quayle p, rutishauser s, vermeirssen elm: monitoring of the ecotoxicological hazard potential by polar organic micropollutants in sewage treatment plants and surface waters using a mode-of-action based test battery. journal of environmental monitoring 2008;10:622-631.
10. garbary dj, miller ag, scrosati r, kim k-y, schofield wb: distribution and salinity tolerance of intertidal mosses from nova scotian salt marshes the bryologist 2008;111:282-191.
11. guidi l, degl’innocenti e: ozone effects on high light-induced photoinhibition in phaseolus vulgaris plant science 2008;174:590-596.
12. hall-spencer jm, rodolfo-metalpa r, martin s, ransome e, fine m, turner sm, rowley sj, tedesco d, buia m-c: volcanic carbon dioxide vents show ecosystem effects of ocean acidification. nature 2008;454:96-99.
13. hideg é: a comparative study of fluorescent singlet oxygen probes in plant leaves central european journal of biology 2008;3:272-284.
14. horst rj, engelsdorf t, sonnewald u, voll lm: infection of maize leaves with ustilago maydis prevents establishment of c4 photosynthesis. journal of plant physiology 2008;165:19-28.
15. krupenina na, bulychev aa, roelfsema mrg, schreiber u: action potential in chara cells intensifies spatial patterns of photosynthetic electron flow and non-photochemical quenching in parallel with inhibition of ph banding. photochemical & photobiological sciences 2008;7:681-688.
16. kuckenberg j, tartachnyk i, noga g: evaluation of fluorescence and remission techniques for monitoring changes in peel chlorophyll and internal fruit characteristics in sunlit and shaded sides of apple fruit during shelf-life postharvest biology and technology 2008;48:231-241.
17. lange pr, geserick c, tischendorf g, zrenner r: functions of chloroplastic adenylate kinases in arabidopsis. plant physiology 2008;146:492-504.
18. lehr n-a, schrey sd, hampp r, tarkka mt: root inoculation with a forest soil streptomycete leads to locally and systemically increased resistance against phytopathogens in norway spruce. new phytologist 2008;177:965-976.
19. liu n, lin z-f, lin g-z, peng c-l, pan x-p, chen s-w: spectral reflectance parameters and pigment functions during leaf ontogenesis in six subtropical landscape plants. plant growth regulation 2008:in press.
20. lu y, savage lj, ajjawi i, imre km, yoder dw, benning c, dellapenna d, ohlrogge jb, osteryoung kw, weber ap, wilkerson cg, last rl: new connections across pathways and cellular processes: industrialized mutant screening reveals novel associations between diverse phenotypes in arabidopsis. plant physiology 2008;146:1482-1500.
21. magnusson m, heimann k, negri ap: comparative effects of herbicides on photosynthesis and growth of tropical estuarine microalgae marine pollution bulletin 2008;56:1545-1552.
22. massacci a, nabiev sm, pietrosanti l, nematov sk, chernikova tn, leipner ktaj: response of the photosynthetic apparatus of cotton (gossypium hirsutum) to the onset of drought stress under field conditions studied by gas-exchange analysis and chlorophyll fluorescence imaging plant physiology and biochemistry 2008;46:189-195.
23. middlebrook r, hoegh-guldberg o, leggat w: the effect of thermal history on the susceptibility of reef-building corals to thermal stress. journal of experimental biology 2008;211:1050-1056.
24. muller r, schreiber u, escher bi, quayle p, nash smb, mueller jf: rapid exposure assessment of psii herbicides in surface water using a novel chlorophyll a fluorescence imaging assay science of the total environment 2008;401:1-3.
25. nicolaisen k, moslavac s, samborski a, valdebenito m, hantke k, maldener i, muro-pastor am, flores e, schleiff e: alr0397 is an outer membrane transporter for the siderophore schizokinen in anabaena sp. strain pcc 7120. journal of bacteriology 2008:in press.
26. pieruschka r, chavarria-krauser a, cloos k, scharr h, schurr u, jahnke s: photosynthesis can be enhanced by lateral co2 diffusion inside leaves over distances of several millimeters. new phytologist 2008;178:335-347.
27. rentzou a, psaras gk: green plastids, maximal psii photochemical efficiency and starch content of inner stem tissues of three mediterranean woody species during the year flora 2008;203:350-357.
28. roff g, kvennefors ece, ulstrup ke, fine m, hoegh-guldberg o: coral disease physiology: the impact of acroporid white syndrome on symbiodinium coral reefs 2008;27:373-377.
29. roff g, ulstrup ke, fine m, ralph pj, hoegh-guldberg o: spatial heterogeneity of photosynthetic activity within diseased corals from the great barrier reef. journal of phycology 2008;44:526-538.
30. shao l, shu z, peng c-l, lin z-f, yang c-w, gu q: enhanced sensitivity of arabidopsis anthocyanin mutants to photooxidation : a study with fluorescence imaging. functional plant biology 2008;35:714-724.
31. shaw cm, lam pks, mueller jf: photosystem ii herbicide pollution in hong kong and its potential photosynthetic effects on corals marine pollution bulletin 2008;57:473-478.
32. 竇新永, 吳國江, 黃紅英, 侯雨佳, 顧群, 彭長連: 麻楓樹幼苗對干旱脅迫的響應. 應用生態學報 2008;19:1425-1430.
33. 李蕓瑛, 竇新永, 彭長連: 三種瀕危木蘭植物幼樹光合特性對高溫的響應. 生態學報 2008;28:1-9.
34. 馬守臣, 徐炳成, 李鳳民, 黃占斌: 冬小麥（triticum aestivum）分蘗冗余生態學意義以及減少冗余對水分利用效率的影響. 生態學報 2008;28:321-326.
35. 施征, 史勝青, 肖文發, 齊力旺: 脫水脅迫對梭梭和胡楊苗葉綠素熒光特性的影響. 林業科學研究 2008;21:566-570.
36. guidi l, mori s, degl"innocenti e, pecchiab s: effects of ozone exposure or fungal pathogen on white lupin leaves as determined by imaging of chlorophyll a fluorescence plant physiology and biochemistry 2007:in press.
37. hennig a, bonfig k, roitsch t, warzecha h: expression of the recombinant bacterial outer surface protein a in tobacco chloroplasts leads to thylakoid localization and loss of photosynthesis. febs journal 2007;274:5749-5758.
38. hideg e, kos pb, vass i: photosystem ii damage induced by chemically generated singlet oxygen in tobacco leaves. physiologia plantarum 2007;131:33-40.
39. hideg é, schreiber u: parallel assessment of ros formation and photosynthesis in leaves by fluorescence imaging. photosynthesis research 2007;92:103-108.
40. lehr na, schrey sd, bauer r, hampp r, tarkka mt: suppression of plant defence response by a mycorrhiza helper bacterium. new phytologist 2007;174:892-903.
41. lenk s, chaerle l, pfündel ee, langsdorf g, hagenbeek d, lichtenthaler hk, van der straeten d, buschmann c: multispectral fluorescence and reflectance imaging at the leaf level and its possible applications. journal of experimental botany 2007;58:807-814.
42. orth t, reumann s, zhang x, fan j, wenzel d, quan s, hu j: the peroxin11 protein family controls peroxisome proliferation in arabidopsis. the plant cell 2007;19:333-350.
43. schreiber u, quayle p, schmidt s, escher bi, mueller jf: methodology and evaluation of a highly sensitive algae toxicity test based on multiwell chlorophyll fluorescence imaging. biosensors and bioelectronics 2007:in press.
44. solymosi k, vitányi b, hideg é, böddi b: etiolation symptoms in sunflower (helianthus annuus) cotyledons partially covered by the pericarp of the achene. annals of botany 2007;99:857-867.
45. ulstrup ke, kühl m, bourne dg: zooxanthellae harvested by ciliates associated with brown band syndrome of corals remain photosynthetically competent. applied and environmental microbiology 2007;73:1968-1975.
46. 鄧培雁, 劉威, 韓博平: 寶山堇菜（viola baoshanensis）鎘脅迫下的光合作用 生態學報 2007;27:1858-1862.
47. 鄧培雁, 劉威, 韓博平, 韓志國: 寶山堇菜（viola baoshanensis）、紫花地丁(v. yedoensis)光合異質性比較 生態學報 2007;27:2983-2989.
48. 鄧培雁, 劉威, 韓志國: 砷脅迫下蜈蚣草光合作用的變化. 生態環境 2007;16:775-778.
49. 高海波, 沈應柏: 用葉綠素熒光研究植物傷害信息的系統性傳遞. 湖北農業科學 2007;46:771-773.
50. aldea m, hamilton jg, resti jp, zangerl ar, berenbaum mr, frank td, delucia eh: comparison of photosynthetic damage from arthropod herbivory and pathogen infection in understory hardwood saplings. oecologia 2006;149:221-232.
51. bonfig kb, schreiber u, gabler a, roitsch t, berger s: infection with virulent and avirulent p. syringae strains differentially affects photosynthesis and sink metabolism in arabidopsis leaves planta 2006;225:1-12.
52. dima e, manetas y, psaras gk: chlorophyll distribution pattern in inner stem tissues: evidence from epifluorescence microscopy and reflectance measurements in 20 woody species trees 2006;20:515-521.
53. escher bi, quayle p, muller r, schreiber u, mueller jf: passive sampling of herbicides combined with effect analysis in algae using a novel high-throughput phytotoxicity assay (maxi-imaging-pam). journal of environmental monitoring 2006;8:456-464.
54. heddad m, norén h, reiser v, dunaeva m, andersson b, adamska i: differential expression and localization of early light-induced proteins in arabidopsis thaliana. plant physiology 2006:in press.
55. hölzl g, witt s, kelly aa, zähringer u, warnecke d, dörmann p, heinz e: functional differences between galactolipids and glucolipids revealed in photosynthesis of higher plants. proc. natl. acad. sci. usa 2006;103:7512-7517.
56. ivanov ag, hendrickson l, krol m, selstam e, öquist g, hurry v, huner npa: digalactosyl-diacylglycerol deficiency impairs the capacity for photosynthetic intersystem electron transport and state transitions in arabidopsis thaliana due to photosystem i acceptor-side limitations. plant cell and physiology 2006;47:1146-1157.
57. kaiser h, grams tee: rapid hydr-opassive opening and subsequent active stomatal closure follow heat-induced electrical signals in mimosa pudica. journal of experimental botany 2006;57:2087-2092.
58. kuster a, altenburger r: development and validation of a new fluorescence-based bioassay for aquatic macrophyte species. chemosphere 2006;67:194-201.
59. lohmann a, schottler ma, brehelin c, kessler f, bock r, cahoon eb, dormann p: deficiency in phylloquinone (vitamin k1) methylation affects prenyl quinone distribution, photosystem i abundance, and anthocyanin accumulation in the arabidopsis atmeng mutant. journal of biological chemistry 2006;281:40461-40472.
60. nagel ka, schurr u, walter a: dynamics of root growth stimulation in nicotiana tabacum in increasing light intensity. plant cell and environment 2006;29:1936-1945.
61. petit a-n, vaillant n, boulay m, clément c, fontaine f: alteration of photosynthesis in grapevines affected by esca. phytopathology 2006;96:1060-1066.
62. pieruschka r, schurr u, jensen m, wolff wf, jahnke s: lateral diffusion of co2 from shaded to illuminated leaf parts affects photosynthesis inside homobaric leaves. new phytologist 2006;169:779-788.
63. swarbrick pj, schulze-lefert p, scholes jd: metabolic consequences of susceptibility and resistance (race-specific and broad-spectrum) in barley leaves challenged with powdery mildew. plant cell and environment 2006;29:1061-1076.
64. vopel k, hawes i: photosynthetic performance of benthic microbial mats in lake hoare, antarctica. limnology and oceanography 2006;51:1801-1812.
65. 蔡馬, 賀立紅, 梁紅: 2種銀杏葉片葉綠素熒光特性的比較. 安徽農業科學 2006;34:3322-3324.
66. 鞏擎柱, 呂金印, 徐柄成, 李鳳民, 張海波: 水分脅迫和種植方式對小麥葉綠素熒光參數及水分利用效率的影響. 西北農林科技大學學報 2006;34:83-87.
67. 郭學民, 王貴禧, 高榮孚, 梁麗松: 果實表皮毛的掃描電鏡觀察及其對果實表面熒光特性的影響. 內蒙古農業大學學報 2006;27:43-47.
68. 賀立紅, 賀立靜, 顧群, 梁紅: 銀杏同一葉片不同部位葉綠素熒光特性的研究. 北方園藝 2006:27-29.
69. 賀立紅, 賀立靜, 梁紅: 銀杏不同品種葉綠素熒光參數的比較. 華南農業大學學報 2006;27:43-46.
70. aldea m, hamilton jg, resti jp, zangerl ar, berenbaum mr, delucia eh: indirect effects of insect herbivory on leaf gas exchange in soybean. plant cell and environment 2005;28:402-411.
71. baek m-h, kim j-h, chung by, kim j-s, lee is: alleviation of salt stress by low dose g-irradiation in rice. biologia plantarum 2005;49:273-276.
72. borisjuk l, nguyen th, neuberger t, rutten t, tschiersch h, claus b, feussner i, webb ag, jakob p, weber h, wobus u, rolletschek h: gradients of lipid storage, photosynthesis and plastid differentiation in developing soybean seeds. new phytologist 2005;163:761-776.
73. gog l, berenbaum mr, delucia eh, zangerl ar: autotoxic effects of essential oils on photosynthesis in parsley, parsnip, and rough lemon. chemoecology 2005;15:115-119.
74. kühl m, chen m, ralph pj, schreiber u, larkum awd: a niche for cyanobacteria containing chlorophyll d. nature 2005;433:820.
75. lautner s, grams tee, matyssek r, fromm j: characteristics of electrical signals in poplar and responses in photosynthesis. plant physiology 2005;138:2200-2209.
76. manetas y, pfanz h: spatial heterogeneity of light penetration through periderm and lenticels and concomitant patchy acclimation of corticular photosynthesis trees 2005;19:409-414.
77. marjanovic z, uwe n, hampp r: mycorrhiza formation enhances adaptive response of hybrid poplar to drought annals of the new york academy of sciences 2005;1048:496-499.
78. podola b, melkonian m: se-lective real-time herbicide monitoring by an array chip biosensor employing diverse microalgae. journal of applied phycology 2005;17:261-271.
79. ralph pj, macinnis-ng cmo, frankart c: fluorescence imaging application: effect of leaf age on seagrass photokinetics. aquatic botany 2005;81:69-84.
80. ralph pj, schreiber u, gademann r, kühl m, larkum awd: coral photobiology studied with a new imaging pulse amplitude modulated fluorometer. journal of phycology 2005;41:335-342.
81. berger s, papadopoulos m, schreiber u, kaiser w, roitsch t: complex regulation of gene expression, photosynthesis and sugar levels by pathogen infection in tomato. physiologia plantarum 2004;122:419-428.
82. hill r, larkum awd, frankart c, kühl m, ralph pj: loss of functional photosystem ii reaction centres in zooxanthellae of corals exposed to bleaching conditions: using fluorescence rise kinetics. photosynthesis research 2004;82:59-72.
83. hill r, schreiber u, gademann r, larkum awd, kühl m, ralph pj: spatial heterogeneity of photosynthesis and the effect of temperature-induced bleaching conditions in three species of corals. marine biology 2004;144:633-640.
84. podola b, nowack ecm, melkonian m: the use of multiple-strain algal sensor chips for the detection and identification of volatile organic compounds. biosensors and bioelectronics 2004;19:1253-1260.
85. koziolek c, grams tee, schreiber u, matyssek r, fromm j: transient knockout of photosynthesis mediated by electrical signals. new phytologist 2003;161:715-722.
86. schreiber u, walz h, kolbowski j: propagation of spatial variations of chlorophyll fluorescence parameters in dandelion leaves induced by spot laser heating. pam news 2003.
采購中心
化工儀器網