27104-- QIAprep Spin Miniprep Kit （50T）
- 公司名稱 北京華夏遠洋科技有限公司
- 品牌 Qiagen/凱杰
- 型號 27104--
- 產地 Qiagen
- 廠商性質 代理商
- 更新時間 2018/5/14 14:33:37
- 訪問次數 897
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QIAprep Spin Miniprep Kit純化分子生物學級別純度質粒DNA，zui高可達 20 μg
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- Product Details
Principle
QIAprep spin columns contain a unique silica gel membrane that binds up to 20 µg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenol-chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. High-purity plasmid DNA eluted from QIAprep spin columns is immediay ready to use - there is no need to precipitate, concentrate, or desalt.
Procedure
Plasmid purification using QIAprep Kits follows a simple bind-wash-elute procedure （see figure "The QIAprep procedure"）. First, bacterial cultures are lysed and the lysates are cleared by centrifugation. The cleared lysates are then applied to the QIAprep module where plasmid DNA adsorbs to the silicagel membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water.
The QIAprep Spin MiniPrep Kit enables purification of up to 20 ug molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning. Versatile QIAprep spin columns can be used either in microcentrifuges or on vacuum manifolds （see figure "QIAprep Spin Column handling options"）. The vacuum procedure provides simplified handling and faster sample processing. QIAprep spin columns can be vacuum processed using the QIAvac 24 plus, QIAvac 6S withQIAvac Luer Adapters, or any other commercial manifold with luer connectors. The QIAprep Spin Miniprep Kit can now also be fully automated on the QIAcube.
| Characteristics | |
| Format | Spin columns |
| Purification module | QIAprep spin columns |
| Throughput | 1–24 samples |
| Preparation time | 24 minipreps in 30 minutes |
| Equipment required | Microcentrifuge or vacuum manifold; fully automatable using the QIAcube |
| Lysate clearing | Centrifugation |
Specifications | |
| Capacity of column reservoir | 800 µl |
| Minimum elution buffer volume | 50 µl |
| Culture volume for high-copy plasmids | 1–5 ml |
| Culture volume for low-copy plasmids/cosmids | 1–10 ml |
Applications
QIAprep Miniprep Kits provide reproducible yields of high-purity DNA suitable for use in most applications, including:
- Automated fluorescent sequencing, including capillary sequencing
- Radioactive sequencing
- Restriction digestion （see figure "Complete digestion with various restriction enzymes"）
- Screening
- Ligation and transformation
- Transfection of robust cells
In addition to plasmid purification from Escherichia coli, QIAprep Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications.
Images
QIAprep Spin Column handling options.
Complete digestion with various restriction enzymes.
QIAprep spin column.
The QIAprep procedure.
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